Organoid models of human and mouse ductal pancreatic cancer.

abstract：:Kit/SCF signaling and Mitf-dependent transcription are both essential for melanocyte development and pigmentation. To identify Mitf-dependent Kit transcriptional targets in primary melanocytes, microarray studies were undertaken. Among identified targets was BCL2, whose germline deletion produces melanocyte loss and w...

journal_title：Cell

authors： McGill GG,Horstmann M,Widlund HR,Du J,Motyckova G,Nishimura EK,Lin YL,Ramaswamy S,Avery W,Ding HF,Jordan SA,Jackson IJ,Korsmeyer SJ,Golub TR,Fisher DE

更新日期：2002-06-14 00:00:00

abstract：:Regulated proteolysis is essential for cell cycle progression in both prokaryotes and eukaryotes. We show here that the ClpXP protease, responsible for the degradation of multiple bacterial proteins, is dynamically localized to specific cellular positions in Caulobacter where it degrades colocalized proteins. The CtrA...

journal_title：Cell

authors： McGrath PT,Iniesta AA,Ryan KR,Shapiro L,McAdams HH

更新日期：2006-02-10 00:00:00

abstract：:Cis-regulatory elements (CREs) are commonly recognized by correlative chromatin features, yet the molecular composition of the vast majority of CREs in chromatin remains unknown. Here, we describe a CRISPR affinity purification in situ of regulatory elements (CAPTURE) approach to unbiasedly identify locus-specific chr...

journal_title：Cell

authors： Liu X,Zhang Y,Chen Y,Li M,Zhou F,Li K,Cao H,Ni M,Liu Y,Gu Z,Dickerson KE,Xie S,Hon GC,Xuan Z,Zhang MQ,Shao Z,Xu J

更新日期：2017-08-24 00:00:00

abstract：:Mosquito-borne flaviviruses, including dengue virus (DENV) and Zika virus (ZIKV), are a growing public health concern. Systems-level analysis of how flaviviruses hijack cellular processes through virus-host protein-protein interactions (PPIs) provides information about their replication and pathogenic mechanisms. We u...

journal_title：Cell

authors： Shah PS,Link N,Jang GM,Sharp PP,Zhu T,Swaney DL,Johnson JR,Von Dollen J,Ramage HR,Satkamp L,Newton B,Hüttenhain R,Petit MJ,Baum T,Everitt A,Laufman O,Tassetto M,Shales M,Stevenson E,Iglesias GN,Shokat L,Tripathi

更新日期：2018-12-13 00:00:00

abstract：:Yeast heat shock protein 104 (Hsp104), the only known eukaryotic disaggregase, remodels both disordered protein aggregates and cross-β sheet amyloids. To handle this diverse clientele, DeSantis et al. report that Hsp104 hexamers use distinct mechanisms-individual subunits are able to dissolve disordered aggregates, bu...

journal_title：Cell

authors： Murray AN,Kelly JW

更新日期：2012-11-09 00:00:00

abstract：:Long-term severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) shedding was observed from the upper respiratory tract of a female immunocompromised individual with chronic lymphocytic leukemia and acquired hypogammaglobulinemia. Shedding of infectious SARS-CoV-2 was observed up to 70 days, and of genomic and s...

journal_title：Cell

authors： Avanzato VA,Matson MJ,Seifert SN,Pryce R,Williamson BN,Anzick SL,Barbian K,Judson SD,Fischer ER,Martens C,Bowden TA,de Wit E,Riedo FX,Munster VJ

更新日期：2020-12-23 00:00:00

abstract：:Insertion/deletion (IDL) mismatches in DNA are lesions consisting of extra bases on one strand. Here, the binding of p53 and its 14 kDa C-terminal domain to DNAs containing one or three 3-cytosine IDL mismatches was examined. Electron microscopy showed that both p53 forms bound predominantly as tetramers at the lesion...

journal_title：Cell

authors： Lee S,Elenbaas B,Levine A,Griffith J

更新日期：1995-06-30 00:00:00

abstract：:G1-specific transcriptional activation by Cln3/CDK initiates the budding yeast cell cycle. To identify targets of Cln3/CDK, we analyzed the SBF and MBF transcription factor complexes by multidimensional protein interaction technology (MudPIT). Whi5 was identified as a stably bound component of SBF but not MBF. Inactiv...

journal_title：Cell

authors： de Bruin RA,McDonald WH,Kalashnikova TI,Yates J 3rd,Wittenberg C

更新日期：2004-06-25 00:00:00

abstract：:Iron is ubiquitous in the environment and in biology. The study of iron biology focuses on physiology and homeostasis-understanding how cells and organisms regulate their iron content, how diverse tissues orchestrate iron allocation, and how dysregulated iron homeostasis leads to common hematological, metabolic, and n...

journal_title：Cell

authors： Hentze MW,Muckenthaler MU,Andrews NC

更新日期：2004-04-30 00:00:00

abstract：:In vertebrates, muscles of the limbs and body wall derive from the lateral compartment of the embryonic somites, and axial muscles derive from the medial compartment. Whereas the mechanisms that direct patterning of somites along the dorsoventral axis are beginning to be understood, little is known about the tissue in...

journal_title：Cell

authors： Pourquié O,Fan CM,Coltey M,Hirsinger E,Watanabe Y,Bréant C,Francis-West P,Brickell P,Tessier-Lavigne M,Le Douarin NM

更新日期：1996-02-09 00:00:00

abstract：:The mammalian BAF complex regulates gene expression by modifying chromatin structure. In this report, we identify 80 genes activated and 2 genes repressed by the BAF complex in SW-13 cells. We find that prior binding of NFI/CTF to the NFI/CTF binding site in CSF1 promoter is required for the recruitment of the BAF com...

journal_title：Cell

authors： Liu R,Liu H,Chen X,Kirby M,Brown PO,Zhao K

更新日期：2001-08-10 00:00:00

abstract：:The role of various segments (gag or v-abl) of the Abelson murine leukemia virus (A-MuLV) genome in both lymphoid cell and fibroblast transformation was examined by deletion of areas from cloned, plasmid DNA representations of the genome. The deleted plasmids were tested by transfection into fibroblasts and by infecti...

journal_title：Cell

authors： Prywes R,Foulkes JG,Rosenberg N,Baltimore D

更新日期：1983-09-01 00:00:00

abstract：:The transmission of information across neuronal synapses is an energetically taxing business. Sheng and colleagues monitored the localization of mitochondria following different levels of synaptic activation and discovered that these organelles change their distribution in interesting ways, stalling near synapses when...

journal_title：Cell

authors： Schuman E,Chan D

更新日期：2004-12-17 00:00:00

abstract：:The removal of apoptotic cells by phagocytic neighbors is essential for metazoan development but remains poorly characterized. Here we report the discovery of a Drosophila phagocytosis receptor, Six-microns-under (SIMU), which is expressed in highly phagocytic cell types during development and required for efficient a...

journal_title：Cell

authors： Kurant E,Axelrod S,Leaman D,Gaul U

更新日期：2008-05-02 00:00:00

abstract：:To determine the extent and nature of genetic variation present in independent isolates of HTLV-III/LAV, the nucleotide sequences of the entire envelope gene and parts of gag and pol were determined for two AIDS viruses. The results indicated that variation throughout the viral genome is extensive and that the envelop...

journal_title：Cell

authors： Starcich BR,Hahn BH,Shaw GM,McNeely PD,Modrow S,Wolf H,Parks ES,Parks WP,Josephs SF,Gallo RC

更新日期：1986-06-06 00:00:00

abstract：:The promoter specificity of transcriptional activators is generally thought to be conferred by the specificity of the DNA-binding domain, which brings the activation domain to the appropriate promoter sequence. We show here, however, that Oct-1 and Oct-2 can differentially activate transcription not through DNA bindin...

journal_title：Cell

authors： Tanaka M,Lai JS,Herr W

更新日期：1992-02-21 00:00:00

abstract：:The molecular mediator and functional significance of meal-associated brown fat (BAT) thermogenesis remains elusive. Here, we identified the gut hormone secretin as a non-sympathetic BAT activator mediating prandial thermogenesis, which consequentially induces satiation, thereby establishing a gut-secretin-BAT-brain a...

journal_title：Cell

authors： Li Y,Schnabl K,Gabler SM,Willershäuser M,Reber J,Karlas A,Laurila S,Lahesmaa M,U Din M,Bast-Habersbrunner A,Virtanen KA,Fromme T,Bolze F,O'Farrell LS,Alsina-Fernandez J,Coskun T,Ntziachristos V,Nuutila P,Klingenspor M

更新日期：2018-11-29 00:00:00

abstract：:The major site of in vivo transcriptional initiation for both heavy and light strands of human mitochondrial DNA is the displacement-loop region. Transcripts synthesized in vitro by human mitochondrial RNA polymerase were mapped to the nucleotide level and have identical 5' end map positions to those reported for in v...

journal_title：Cell

authors： Chang DD,Clayton DA

更新日期：1984-03-01 00:00:00

abstract：:Gene silencing is instrumental to interrogate gene function and holds promise for therapeutic applications. Here, we repurpose the endogenous retroviruses' silencing machinery of embryonic stem cells to stably silence three highly expressed genes in somatic cells by epigenetics. This was achieved by transiently expres...

journal_title：Cell

authors： Amabile A,Migliara A,Capasso P,Biffi M,Cittaro D,Naldini L,Lombardo A

更新日期：2016-09-22 00:00:00

abstract：:The yeast Sir2/3/4p complex is found in abundance at telomeres, where it participates in the formation of silent heterochromatin and telomere maintenance. Here, we show that Sir3p is released from telomeres in response to DNA double-strand breaks (DSBs), binds to DSBs, and mediates their repair, independent of cell ma...

journal_title：Cell

authors： Mills KD,Sinclair DA,Guarente L

更新日期：1999-05-28 00:00:00

abstract：:Choices have consequences. Immune cells survey and migrate throughout the body and sometimes take residence in niche environments with distinct communities of cells, extracellular matrix, and nutrients that may differ from those in which they matured. Imbedded in immune cell physiology are metabolic pathways and metab...

journal_title：Cell

authors： Buck MD,Sowell RT,Kaech SM,Pearce EL

更新日期：2017-05-04 00:00:00

abstract：:NRK fibroblasts exposed to transforming growth factor-beta (TGF-beta) show increased binding of radiolabeled epidermal growth factor (EGF) relative to untreated cells. The binding of another growth factor, rat insulin-like growth factor-II, is unaffected. The increase in EGF binding induced by TGF-beta is not due to i...

journal_title：Cell

authors： Assoian RK,Frolik CA,Roberts AB,Miller DM,Sporn MB

更新日期：1984-01-01 00:00:00

abstract：:Plants greatly rely on their root microbiome for uptake of nutrients and protection against stresses. Recent studies have uncovered the involvement of plant stress responses in the assembly of plant-beneficial microbiomes. To facilitate durable crop production, deciphering the driving forces that shape the microbiome ...

journal_title：Cell

authors： Bakker PAHM,Pieterse CMJ,de Jonge R,Berendsen RL

更新日期：2018-03-08 00:00:00

abstract：:The X-linked Foxp3 is a member of the forkhead/winged helix transcription factor family. Germline mutations cause lethal autoimmune diseases in males. Serendipitously, we observed that female mice heterozygous for the "scurfin" mutation of the Foxp3 gene (Foxp3(sf/+)) developed cancer at a high rate. The majority of t...

journal_title：Cell

authors： Zuo T,Wang L,Morrison C,Chang X,Zhang H,Li W,Liu Y,Wang Y,Liu X,Chan MW,Liu JQ,Love R,Liu CG,Godfrey V,Shen R,Huang TH,Yang T,Park BK,Wang CY,Zheng P,Liu Y

更新日期：2007-06-29 00:00:00

abstract：:DNA transcription, replication, and repair are regulated by histone acetylation, a process that requires the generation of acetyl-coenzyme A (CoA). Here, we show that all the subunits of the mitochondrial pyruvate dehydrogenase complex (PDC) are also present and functional in the nucleus of mammalian cells. We found t...

journal_title：Cell

authors： Sutendra G,Kinnaird A,Dromparis P,Paulin R,Stenson TH,Haromy A,Hashimoto K,Zhang N,Flaim E,Michelakis ED

更新日期：2014-07-03 00:00:00

abstract：:We have identified an essential gene, called FIP1, encoding a 327 amino acid protein interacting with yeast poly(A) polymerase (PAP1) in the two-hybrid assay. Recombinant FIP1 protein forms a 1:1 complex with PAP1 in vitro. At 37 degrees C, a thermosensitive allele of FIP1 shows a shortening of poly(A) tails and a dec...

journal_title：Cell

authors： Preker PJ,Lingner J,Minvielle-Sebastia L,Keller W

更新日期：1995-05-05 00:00:00

abstract：:Most inducible transcriptional programs consist of primary and secondary response genes (PRGs and SRGs) that differ in their kinetics of expression and in their requirements for new protein synthesis and chromatin remodeling. Here we show that many PRGs, in contrast to SRGs, have preassembled RNA polymerase II (Pol II...

journal_title：Cell

authors： Hargreaves DC,Horng T,Medzhitov R

更新日期：2009-07-10 00:00:00

abstract：:Drosophila bicoid mRNA is synthesized in the nurse cells and transported to the oocyte where microtubules and Exuperantia protein mediate localization to the anterior pole. Fluorescent bicoid mRNA injected into the oocyte displays nonpolar microtubule-dependent transport to the closest cortical surface, and the oocyte...

journal_title：Cell

authors： Cha BJ,Koppetsch BS,Theurkauf WE

更新日期：2001-07-13 00:00:00

abstract：:The association of histone modification changes with autism spectrum disorder (ASD) has not been systematically examined. We conducted a histone acetylome-wide association study (HAWAS) by performing H3K27ac chromatin immunoprecipitation sequencing (ChIP-seq) on 257 postmortem samples from ASD and matched control brai...

journal_title：Cell

authors： Sun W,Poschmann J,Cruz-Herrera Del Rosario R,Parikshak NN,Hajan HS,Kumar V,Ramasamy R,Belgard TG,Elanggovan B,Wong CCY,Mill J,Geschwind DH,Prabhakar S

更新日期：2016-11-17 00:00:00

abstract：:Regeneration is one of the great mysteries of biology. Planarians are flatworms capable of dramatic feats of regeneration, which have been studied for over 2 centuries. Recent findings identify key cellular and molecular principles underlying these feats. A stem cell population (neoblasts) generates new cells and is c...

journal_title：Cell

authors： Reddien PW

更新日期：2018-10-04 00:00:00