当前位置： SCI文献检索 > CELL期刊下所有文献 > Adaptation in bacterial chemotaxis: CheB-dependent modification permits additional methylations of sensory transducer proteins.

Adaptation in bacterial chemotaxis: CheB-dependent modification permits additional methylations of sensory transducer proteins.

Abstract：

:Sensory transduction in E. coli consists of two phases, excitation and adaptation, both of which involve the methyl-accepting chemotaxis proteins (MCPs). These molecules relay transmembrane signals and are reversibly methylated during adaptation of E. coli to environmental stimuli. Each MCP contains multiple sites of methylation, and we identified six of these sites in MCPI. Recently, a second covalent modification of MCPs has been identified, which is not methylation. This modification, designated CheB-dependent modification, is stimulated by repellents and causes a net increase in the negative charge of MCPI and MCPII by one or two charges. We demonstrate that one CheB modification occurs on the methyl-accepting methionine-and lysine-containing tryptic peptide in MCPI and MCPII, and the second CheB modification is on an arginine-containing tryptic peptide. The CheB modification allows three additional methyl groups to be incorporated into the methyl-accepting methionine-lysine peptide, while not actually creating all of these methylation sites. The two CheB modifications occur sequentially. A possible mechanism by which CheB modification permits additional methylations and the role of CheB modification in bacterial chemotaxis are discussed.

journal_name

Cell

journal_title

Cell

authors

Kehry MR,Dahlquist FW

doi

10.1016/0092-8674(82)90438-x

subject

Has Abstract

pub_date

1982-07-01 00:00:00

pages

761-72

issue

3

eissn

0092-8674

issn

1097-4172

pii

0092-8674(82)90438-X

journal_volume

29

pub_type

杂志文章

相关文献

CELL文献大全
  • Interaction domains of Sos1/Grb2 are finely tuned for cooperative control of embryonic stem cell fate.

    abstract：:Metazoan evolution involves increasing protein domain complexity, but how this relates to control of biological decisions remains uncertain. The Ras guanine nucleotide exchange factor (RasGEF) Sos1 and its adaptor Grb2 are multidomain proteins that couple fibroblast growth factor (FGF) signaling to activation of the R...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/j.cell.2013.01.056

    authors： Findlay GM,Smith MJ,Lanner F,Hsiung MS,Gish GD,Petsalaki E,Cockburn K,Kaneko T,Huang H,Bagshaw RD,Ketela T,Tucholska M,Taylor L,Bowtell DD,Moffat J,Ikura M,Li SS,Sidhu SS,Rossant J,Pawson T

    更新日期：2013-02-28 00:00:00

  • All-Optical Electrophysiology Reveals the Role of Lateral Inhibition in Sensory Processing in Cortical Layer 1.

    abstract：:Cortical layer 1 (L1) interneurons have been proposed as a hub for attentional modulation of underlying cortex, but the transformations that this circuit implements are not known. We combined genetically targeted voltage imaging with optogenetic activation and silencing to study the mechanisms underlying sensory proce...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/j.cell.2020.01.001

    authors： Fan LZ,Kheifets S,Böhm UL,Wu H,Piatkevich KD,Xie ME,Parot V,Ha Y,Evans KE,Boyden ES,Takesian AE,Cohen AE

    更新日期：2020-02-06 00:00:00

  • Progressive loss of shape-responsive metabolic controls in cells with increasingly transformed phenotype.

    abstract：:The modulation of cell metabolism by cell shape and external surface contact has been studied by suspension culture of anchorage-dependent fibroblasts. The suspended cells shut down protein synthesis and nuclear RNA metabolism and cease replicating DNA. However, these responses to suspension are lost or modified as ce...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/0092-8674(81)90111-2

    authors： Wittelsberger SC,Kleene K,Penman S

    更新日期：1981-06-01 00:00:00

  • Phosphorylation-regulated binding of RNA polymerase II to fibrous polymers of low-complexity domains.

    abstract：:The low-complexity (LC) domains of the products of the fused in sarcoma (FUS), Ewings sarcoma (EWS), and TAF15 genes are translocated onto a variety of different DNA-binding domains and thereby assist in driving the formation of cancerous cells. In the context of the translocated fusion proteins, these LC sequences fu...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/j.cell.2013.10.033

    authors： Kwon I,Kato M,Xiang S,Wu L,Theodoropoulos P,Mirzaei H,Han T,Xie S,Corden JL,McKnight SL

    更新日期：2013-11-21 00:00:00

  • Ulcerative colitis-like disease in mice with a disrupted interleukin-2 gene.

    abstract：:Mice deficient for interleukin-2 develop normally during the first 3-4 weeks of age. However, later on they become severely compromised, and about 50% of the animals die between 4 and 9 weeks after birth. Of the remaining mice, 100% develop an inflammatory bowel disease with striking clinical and histological similari...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/0092-8674(93)80067-o

    authors： Sadlack B,Merz H,Schorle H,Schimpl A,Feller AC,Horak I

    更新日期：1993-10-22 00:00:00

  • The homeobox gene goosecoid controls cell migration in Xenopus embryos.

    abstract：:Goosecoid (gsc), a homeobox gene expressed specifically in the dorsal blastopore lip of the Xenopus gastrula, is considered to play an important role in Spemann's organizer phenomenon. Lineage tracing and time-lapse microscopy were used to follow the fate of embryonic cells microinjected with gsc mRNA. Microinjected g...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/0092-8674(93)90069-3

    authors： Niehrs C,Keller R,Cho KW,De Robertis EM

    更新日期：1993-02-26 00:00:00

  • Each of the activities of signal recognition particle (SRP) is contained within a distinct domain: analysis of biochemical mutants of SRP.

    abstract：:Signal recognition particle (SRP), a small ribonucleoprotein required for targeting secretory proteins to the ER, has three known functions: signal recognition, elongation arrest, and translocation promotion. Because SRP is inactivated by the sulfhydryl alkylating reagent N-ethylmaleimide (NEM), we have attempted to e...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/0092-8674(88)90529-6

    authors： Siegel V,Walter P

    更新日期：1988-01-15 00:00:00

  • Nucleotide sequence of Xenopus borealis oocyte 5S DNA: comparison of sequences that flank several related eucaryotic genes.

    abstract：:Genomic Xenopus borealis oocyte-specific 5S DNA (Xbo) contains clusters of 5S rRNA genes. The number of genes varies among clusters, and the distance between genes within a cluster is about 80 nucleotides. The spacer DNA between gene clusters is AT-rich and heterogeneous in length due in part to variable numbers of a ...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/0092-8674(78)90042-9

    authors： Korn LJ,Brown DD

    更新日期：1978-12-01 00:00:00

  • IkappaB kinase promotes tumorigenesis through inhibition of forkhead FOXO3a.

    abstract：:Nuclear exclusion of the forkhead transcription factor FOXO3a by protein kinase Akt contributes to cell survival. We investigated the pathological relationship between phosphoylated-Akt (Akt-p) and FOXO3a in primary tumors. Surprisingly, FOXO3a was found to be excluded from the nuclei of some tumors lacking Akt-p, sug...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/s0092-8674(04)00302-2

    authors： Hu MC,Lee DF,Xia W,Golfman LS,Ou-Yang F,Yang JY,Zou Y,Bao S,Hanada N,Saso H,Kobayashi R,Hung MC

    更新日期：2004-04-16 00:00:00

  • ARIA, a protein that stimulates acetylcholine receptor synthesis, is a member of the neu ligand family.

    abstract：:Motor neurons stimulate their postsynaptic muscle targets to synthesize neurotransmitter receptors. Polypeptide signaling molecules may mediate this inductive interaction. Here we report the purification of ARIA, a protein that stimulates the synthesis of muscle acetylcholine receptors, and the isolation of ARIA cDNA....

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/0092-8674(93)90407-h

    authors： Falls DL,Rosen KM,Corfas G,Lane WS,Fischbach GD

    更新日期：1993-03-12 00:00:00

  • Integration sites and sequence arrangement of SV40 DNA in a homogeneous series of transformed rat fibroblast lines.

    abstract：:The state and organization of viral DNA sequences present in independently isolated rat cell lines transformed with SV40 were investigated using restriction-enzyme cleavage of the cellular DNA and blot hybridization with a viral probe. The transformed lines were established under conditions as identical as possible, e...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/0092-8674(80)90569-3

    authors： Mougneau E,Birg F,Rassoulzadegan M,Cuzin F

    更新日期：1980-12-01 00:00:00

  • Managing to excel at science.

    abstract：:Amid growing recognition that a successful scientific career requires skills beyond scientific acumen, institutions are racing to provide management training for newly minted principal investigators. ...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/j.cell.2008.03.008

    authors： Aschwanden C

    更新日期：2008-03-21 00:00:00

  • A mechanism for gene-environment interaction in the etiology of congenital scoliosis.

    abstract：:Congenital scoliosis, a lateral curvature of the spine caused by vertebral defects, occurs in approximately 1 in 1,000 live births. Here we demonstrate that haploinsufficiency of Notch signaling pathway genes in humans can cause this congenital abnormality. We also show that in a mouse model, the combination of this g...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/j.cell.2012.02.054

    authors： Sparrow DB,Chapman G,Smith AJ,Mattar MZ,Major JA,O'Reilly VC,Saga Y,Zackai EH,Dormans JP,Alman BA,McGregor L,Kageyama R,Kusumi K,Dunwoodie SL

    更新日期：2012-04-13 00:00:00

  • Higher-order assemblies in a new paradigm of signal transduction.

    abstract：:Recent studies have revealed that multiple intracellular signaling proteins may assemble into structured, yet sometimes infinite, higher-order signaling machines for transmission of receptor activation information to cellular responses. These studies advance our understanding of cell signaling and implicate new molecu...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/j.cell.2013.03.013

    authors： Wu H

    更新日期：2013-04-11 00:00:00

  • The yeast spindle pole body is assembled around a central crystal of Spc42p.

    abstract：:The spindle pole body (SPB) is the microtubule organizing center (MTOC) in the yeast Saccharomyces that plays a pivotal role in such diverse processes as mitosis, budding, and mating. We have used cryoelectron microscopy and image processing to study the structure of isolated diploid SPBs. We show that SPBs are presen...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/s0092-8674(00)80295-0

    authors： Bullitt E,Rout MP,Kilmartin JV,Akey CW

    更新日期：1997-06-27 00:00:00

  • Sequence from early region of polyoma virus DNA containing viral replication origin and encoding small, middle and (part of) large T antigens.

    abstract：:The sequence of about one third of the polyoma virus genome is presented. This sequence covers the origin of replication of two large plaque strains (A2 and A3) of polyoma virus. The two strains differ by 11 bp in the origin region. A model for replication is suggested. The sequence probably also covers the entire cod...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/0092-8674(79)90162-4

    authors： Soeda E,Arrand JR,Smolar N,Griffin BE

    更新日期：1979-06-01 00:00:00

  • Munc13-4 is essential for cytolytic granules fusion and is mutated in a form of familial hemophagocytic lymphohistiocytosis (FHL3).

    abstract：:Secretion of cytolytic granules content at the immunological synapse is a highly regulated process essential for lymphocyte cytotoxicity. This process requires the rapid transfer of perforin containing lytic granules to the target cell interface, followed by their docking and fusion with the plasma membrane. Defective...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/s0092-8674(03)00855-9

    authors： Feldmann J,Callebaut I,Raposo G,Certain S,Bacq D,Dumont C,Lambert N,Ouachée-Chardin M,Chedeville G,Tamary H,Minard-Colin V,Vilmer E,Blanche S,Le Deist F,Fischer A,de Saint Basile G

    更新日期：2003-11-14 00:00:00

  • Intron sequences involved in lariat formation during pre-mRNA splicing.

    abstract：:We have shown that lariat formation during in vitro splicing of several RNA precursors, from Drosophila to man, occurs at a unique and identifiable but weakly conserved site, 18 to 37 nucleotides proximal to the 3' splice site. Lariat formation within an artificial intron lacking a normal branch-point sequence occurs ...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/0092-8674(85)90064-9

    authors： Reed R,Maniatis T

    更新日期：1985-05-01 00:00:00

  • Architecture of a coat for the nuclear pore membrane.

    abstract：:The symmetric core of the nuclear pore complex can be considered schematically as a series of concentric cylinders. A peripheral cylinder coating the pore membrane contains the previously characterized, elongated heptamer that harbors Sec13-Nup145C in its middle section. Strikingly, Sec13-Nup145C crystallizes as a het...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/j.cell.2007.11.038

    authors： Hsia KC,Stavropoulos P,Blobel G,Hoelz A

    更新日期：2007-12-28 00:00:00

  • An active tissue-specific enhancer and bound transcription factors existing in a precisely positioned nucleosomal array.

    abstract：:Nucleosomes positioned over promoters are usually inhibitory to protein binding and activity. We analyzed at the nucleotide level of resolution the nucleosomal organization of a distal, liver-specific enhancer in various mouse tissues and found that the enhancer exists in an array of three precisely positioned nucleos...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/0092-8674(93)80079-t

    authors： McPherson CE,Shim EY,Friedman DS,Zaret KS

    更新日期：1993-10-22 00:00:00

  • Widespread Proteome Remodeling and Aggregation in Aging C. elegans.

    abstract：:Aging has been associated with a progressive decline of proteostasis, but how this process affects proteome composition remains largely unexplored. Here, we profiled more than 5,000 proteins along the lifespan of the nematode C. elegans. We find that one-third of proteins change in abundance at least 2-fold during agi...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/j.cell.2015.03.032

    authors： Walther DM,Kasturi P,Zheng M,Pinkert S,Vecchi G,Ciryam P,Morimoto RI,Dobson CM,Vendruscolo M,Mann M,Hartl FU

    更新日期：2015-05-07 00:00:00

  • The transcription map of mouse mitochondrial DNA.

    abstract：:Nine transcripts complementary to mouse L cell mitochondrial DNA have been detected, sized and mapped to restriction fragments using the method of Berk and Sharp (1977). RNA isolated from L cell mitochondria was hybridized to 32P-labeled, cloned L cell mitochondrial DNA restriction fragments in 70% formamide under con...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/0092-8674(78)90309-4

    authors： Battey J,Clayton DA

    更新日期：1978-05-01 00:00:00

  • DNA sequence organization of the beta-globin complex in the BALB/c mouse.

    abstract：:Clones containing five non-adult beta-globin genes were isolated from a library of BALB/c DNA. Together, the newly cloned regions comprise a contiguous block of 32 kb of the mouse genome. Restriction mapping of genomic DNA established the physical linkage of these non-adult genes to the two adult beta-globin genes as ...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/0092-8674(80)90123-3

    authors： Jahn CL,Hutchison CA 3rd,Phillips SJ,Weaver S,Haigwood NL,Voliva CF,Edgell MH

    更新日期：1980-08-01 00:00:00

  • Cooperativity in transactivation between retinoic acid receptor and TFIID requires an activity analogous to E1A.

    abstract：:In embryonal carcinoma (EC) cells retinoic acid (RA) strongly induces transcription from the RA receptor beta 2 (RAR beta 2) promoter through an RA response element (RARE) located in close proximity to the TATA box. Here we demonstrate that recombinant human TATA box-binding protein, hTFIID, and RAR functionally coope...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/0092-8674(92)90443-g

    authors： Berkenstam A,Vivanco Ruiz MM,Barettino D,Horikoshi M,Stunnenberg HG

    更新日期：1992-05-01 00:00:00

  • Immunogenic structure of the influenza virus hemagglutinin.

    abstract：:We chemically synthesized 20 peptides corresponding to 75% of the HA1 molecule of the influenza virus. Antibodies to the majority (18) of these peptides were capable of reacting with the hemagglutinin molecule. These 18 peptides are not confined to the known antigenic determinants of the hemagglutinin molecule, but ra...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/0092-8674(82)90202-1

    authors： Green N,Alexander H,Olson A,Alexander S,Shinnick TM,Sutcliffe JG,Lerner RA

    更新日期：1982-03-01 00:00:00

  • Yeast histone H4 N-terminal sequence is required for promoter activation in vivo.

    abstract：:To search for histone domains that may regulate transcription in vivo, we made deletions and amino acid substitutions in the histone N-termini of S. cerevisiae. Histone H4 N-terminal residues 4-23, which include the extremely conserved, reversibly acetylated lysines (at positions 5, 8, 12, and 16), were found to encom...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/0092-8674(91)90554-c

    authors： Durrin LK,Mann RK,Kayne PS,Grunstein M

    更新日期：1991-06-14 00:00:00

  • Ubiquitylation of the 9-1-1 checkpoint clamp is independent of rad6-rad18 and DNA damage.

    abstract：:A recent report proposed a function of the ubiquitin conjugation factors Rad6 and Rad18 comparable to the bacterial SOS response, controlling damage-induced transcriptional activation and contributing to checkpoint signaling. The relevant ubiquitylation target was identified as budding yeast Rad17, a subunit of the PC...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/j.cell.2010.04.039

    authors： Davies AA,Neiss A,Ulrich HD

    更新日期：2010-06-11 00:00:00

  • Circular Concatemers of Ultra-Short DNA Segments Produce Regulatory RNAs.

    abstract：:In the ciliated protozoan Paramecium tetraurelia, Piwi-associated small RNAs are generated upon the elimination of tens of thousands of short transposon-derived DNA segments as part of development. These RNAs then target complementary DNA for elimination in a positive feedback process, contributing to germline defense...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/j.cell.2017.02.020

    authors： Allen SE,Hug I,Pabian S,Rzeszutek I,Hoehener C,Nowacki M

    更新日期：2017-03-09 00:00:00

  • A Living Biobank of Breast Cancer Organoids Captures Disease Heterogeneity.

    abstract：:Breast cancer (BC) comprises multiple distinct subtypes that differ genetically, pathologically, and clinically. Here, we describe a robust protocol for long-term culturing of human mammary epithelial organoids. Using this protocol, >100 primary and metastatic BC organoid lines were generated, broadly recapitulating t...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/j.cell.2017.11.010

    authors： Sachs N,de Ligt J,Kopper O,Gogola E,Bounova G,Weeber F,Balgobind AV,Wind K,Gracanin A,Begthel H,Korving J,van Boxtel R,Duarte AA,Lelieveld D,van Hoeck A,Ernst RF,Blokzijl F,Nijman IJ,Hoogstraat M,van de Ven M,Egan

    更新日期：2018-01-11 00:00:00

  • Molecular cloning and functional analysis of Drosophila TAF110 reveal properties expected of coactivators.

    abstract：:The general transcription factor TFIID is a multiprotein complex containing the TATA-binding protein and several associated factors (TAFs), some of which may function as coactivators that are essential for activated, but not basal, transcription. Here we describe the isolation and characterization of the first gene en...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/0092-8674(93)90664-c

    authors： Hoey T,Weinzierl RO,Gill G,Chen JL,Dynlacht BD,Tjian R

    更新日期：1993-01-29 00:00:00