Abstract:
:Signal recognition particle (SRP), a small ribonucleoprotein required for targeting secretory proteins to the ER, has three known functions: signal recognition, elongation arrest, and translocation promotion. Because SRP is inactivated by the sulfhydryl alkylating reagent N-ethylmaleimide (NEM), we have attempted to establish structure-function relationships within SRP by assembling particles in which a single protein is modified. Alkylation of the 68/72 kd protein of SRP yields a particle that arrests elongation but fails to promote translocation and no longer interacts with SRP receptor. Alkylation of the 54 kd protein yields a particle that fails to recognize signal sequences. This approach has allowed us to map activities to specific protein domains on SRP, and should be generally useful for analyzing other ribonucleoproteins.
journal_name
Celljournal_title
Cellauthors
Siegel V,Walter Pdoi
10.1016/0092-8674(88)90529-6subject
Has Abstractpub_date
1988-01-15 00:00:00pages
39-49issue
1eissn
0092-8674issn
1097-4172pii
0092-8674(88)90529-6journal_volume
52pub_type
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