Abstract：
:Basal cells are by definition located on the basolateral side of several epithelia, and they have never been observed reaching the lumen. Using high-resolution 3D confocal imaging, we report that basal cells extend long and slender cytoplasmic projections that not only reach toward the lumen but can cross the tight junction barrier in some epithelia of the male reproductive and respiratory tracts. In this way, the basal cell plasma membrane is exposed to the luminal environment. In the epididymis, in which luminal acidification is crucial for sperm maturation and storage, these projections contain the angiotensin II type 2 receptor (AGTR2). Activation of AGTR2 by luminal angiotensin II, increases proton secretion by adjacent clear cells, which are devoid of AGTR2. We propose a paradigm in which basal cells scan and sense the luminal environment of pseudostratified epithelia and modulate epithelial function by a mechanism involving crosstalk with other epithelial cells.
journal_name
Celljournal_title
Cellauthors
Shum WW,Da Silva N,McKee M,Smith PJ,Brown D,Breton Sdoi
10.1016/j.cell.2008.10.020subject
Has Abstractpub_date
2008-12-12 00:00:00pages
1108-17issue
6eissn
0092-8674issn
1097-4172pii
S0092-8674(08)01312-3journal_volume
135pub_type
杂志文章相关文献
CELL文献大全abstract：:Resident proteins of the ER lumen carry a specific tetrapeptide signal (KDEL or HDEL) that prevents their secretion. We have previously described the isolation of yeast mutants that fail to retain such resident proteins within the cell. Here we describe ERD2, a gene required for retention. It encodes a 26 kd integral ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(90)90698-e
更新日期：1990-06-29 00:00:00
abstract：:The frequencies of transformations of primary human and Chinese hamster fibroblasts have been compared with the spontaneous and induced frequencies of mutation for resistance to thioguanine and ouabain, and for ability to use fructose, using the carcinogens benzo (alpha) pyrene and urethane. Whereas the rates and freq...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(78)90215-5
更新日期：1978-04-01 00:00:00
abstract：:ATP-dependent proteases are vital to maintain cellular protein homeostasis. Here, we study the mechanisms of force generation and intersubunit coordination in the ClpXP protease from E. coli to understand how these machines couple ATP hydrolysis to mechanical protein unfolding. Single-molecule analyses reveal that pho...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2013.09.022
更新日期：2013-10-24 00:00:00
abstract：:Replication of Escherichia coli plasmid p15A was examined by use of a cell extract or a mixture of three purified E. coli enzymes: RNA polymerase; RNAase H; and DNA polymerase I. In each system, replication initiates at any of three consecutive nucleotides located at a unique site. Primer transcription starts 508 bp u...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(83)90502-0
更新日期：1983-01-01 00:00:00
abstract：:Mice homozygous for the recessive mutation swaying (sw) are characterized by ataxia and hypertonia, attributed to the malformation of anterior regions of the cerebellum. We show that sw is a deletion of a single base pair from the proto-oncogene Wnt-1. The deletion is predicted to cause premature termination of transl...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(91)90369-a
更新日期：1991-11-29 00:00:00
abstract：:Hybrids between mouse hepatoma cells (which secrete several serum proteins) and mouse or rat fibroblasts (which do not secrete these proteins) produce transferrin and the third component of complement (C3) like the parental hepatoma cells, while they do not secrete either albumin or alpha-fetoprotein (AFP). This lack ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(75)90073-2
更新日期：1975-09-01 00:00:00
abstract：:Bacteria acquire memory of viral invaders by incorporating invasive DNA sequence elements into the host CRISPR locus, generating a new spacer within the CRISPR array. We report on the structures of Cas1-Cas2-dual-forked DNA complexes in an effort toward understanding how the protospacer is sampled prior to insertion i...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2015.10.008
更新日期：2015-11-05 00:00:00
abstract：:Transcriptional regulation by the glucocorticoid receptor (GR) is mediated by hormone binding, receptor dimerization, and coactivator recruitment. Here, we report the crystal structure of the human GR ligand binding domain (LBD) bound to dexamethasone and a coactivator motif derived from the transcriptional intermedia...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(02)00817-6
更新日期：2002-07-12 00:00:00
abstract：:Many biological motor molecules move within cells using stepsizes predictable from their structures. Myosin VI, however, has much larger and more broadly distributed stepsizes than those predicted from its short lever arms. We explain the discrepancy by monitoring Qdots and gold nanoparticles attached to the myosin-VI...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2010.08.033
更新日期：2010-09-17 00:00:00
abstract：:The small ribonucleoproteins recognized by anti-La autoantibodies contain a heterogeneous mixture of small RNAs from uninfected mammalian cells. The identity of many of these has now been established by the discovery of precursor forms of 5S rRNA and of certain tRNAs among La RNAs from HeLa cells. The small fraction o...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(82)90099-x
更新日期：1982-05-01 00:00:00
abstract：:The mitotic checkpoint prevents advance to anaphase prior to successful attachment of every centromere/kinetochore to mitotic spindle microtubules. Using purified components and Xenopus egg extracts, the kinetochore-associated microtubule motor CENP-E is now shown to be the activator of the essential checkpoint kinase...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(03)00475-6
更新日期：2003-07-11 00:00:00
abstract：:c-Myc (Myc) and Max proteins dimerize and bind DNA through basic-helix-loop-helix-leucine zipper motifs (b-HLH-LZ). Using a genetic approach, we demonstrate that binding to Max is essential for Myc transforming activity and that Myc homodimers are inactive. Mutants of Myc and Max that bind efficiently to each other bu...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(93)90663-b
更新日期：1993-01-29 00:00:00
abstract：:We have localized the regulatory sequence required for viral or poly(I)-poly(C) activation of human beta-interferon gene expression to a region located between -37 and -77 from the mRNA cap site. This sequence has the characteristics of an inducible enhancer element: it can act upstream or downstream of the beta-inter...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(85)80024-6
更新日期：1985-06-01 00:00:00
abstract：:A strong transcription enhancer was identified in the genomic DNA (235 kb) of human cytomegalovirus (HCMV), a ubiquitous and severe pathogen of the herpesvirus group. Cotransfection of enhancerless SV40 DNA with randomly fragmented HCMV DNA yielded two SV40-HCMV recombinant viruses that had incorporated overlapping se...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(85)80025-8
更新日期：1985-06-01 00:00:00
abstract：:The transcription factor NRF2 is a master regulator of the cellular antioxidant response, and it is often genetically activated in non-small-cell lung cancers (NSCLCs) by, for instance, mutations in the negative regulator KEAP1. While direct pharmacological inhibition of NRF2 has proven challenging, its aberrant activ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2017.08.051
更新日期：2017-10-19 00:00:00
abstract：:During the postpartum period, the brain's inhibitory GABAA receptors may not recover in time following their reduced numbers during pregnancy. This is likely the cause of postpartum depression prevalent in ∼12% of childbearing women. A new therapy for this condition consists of administering a synthetic neurosteroid d...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2018.12.016
更新日期：2019-01-10 00:00:00
abstract：:Cultured NIH 3T3 cells devoid of endogenous EGF receptors were transfected with cDNA constructs encoding either the human EGF receptor or an EGF receptor mutant in which Lys721, a key residue in the ATP binding site, was replaced with an alanine residue. The mutant receptor was properly processed, and it displayed bot...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(87)90147-4
更新日期：1987-10-23 00:00:00
abstract：:How is the characteristic shape of an organelle generated? Recent work has provided insight into how the tubular network of the endoplasmic reticulum (ER) is formed. The tubules themselves are shaped by the reticulons and DP1/Yop1p, whereas their fusion into a network is brought about by membrane-bound GTPases that in...
journal_title：Cell
pub_type： 杂志文章,评审
doi：10.1016/j.cell.2011.11.022
更新日期：2011-12-09 00:00:00
abstract：:Removal of histone H1 produces a transition in the structure of chromatin fibers as observed by electron microscopy. Chromatin containing all histone proteins appears as fibers with a diameter of about 250 A. The nucleosomes within these fibers are closely packed. If histone H1 is selectively removed with 50-100 mM Na...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(77)90188-x
更新日期：1977-09-01 00:00:00
abstract：:We have performed in vitro kinetic analyses of transcription from the 5S gene promoter in order to resolve the rate-limiting events which lead to accurate transcription of the 5S RNA gene. We demonstrate that a rate-limiting intermediate can be formed during an extended incubation prior to initiation of transcription....
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(85)90315-0
更新日期：1985-01-01 00:00:00
abstract：:Stable myoblast cell lines were isolated after a brief exposure of mouse fibroblasts (10T1/2 cells) to 5-azacytidine. We show that transfection of 10T1/2 cells with DNA from these azacytidine-induced myoblasts (or from mouse C2C12 myoblasts) results in myogenic conversion of approximately 1 in 15,000 transfected colon...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(86)90507-6
更新日期：1986-12-05 00:00:00
abstract：:Sixty-one codons specify 20 amino acids, offering cells many options for encoding a polypeptide sequence. Two new studies (Cannarrozzi et al., 2010; Tuller et al., 2010) now foster the idea that patterns of codon usage can control ribosome speed, fine-tuning translation to increase the efficiency of protein synthesis....
journal_title：Cell
pub_type： 评论,杂志文章,评审
doi：10.1016/j.cell.2010.03.033
更新日期：2010-04-16 00:00:00
abstract：:We have localized ten oxi3- mutations in the first, al1, intron of the coxl gene. All are splicing deficient, being unable to excise the intron. Complementation experiments disclose several domains in the intron al1: the 5'-proximal and 3'-proximal domains harbor cis-dominant mutations, while trans-recessive ones are ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(83)90106-x
更新日期：1983-12-01 00:00:00
abstract：:Cells are devices whose structures delimit function. For example, in the nervous system, neuronal and glial shapes dictate paths of information flow. To understand how cells acquire their shapes, we examined the formation of a sense organ in C. elegans. Using time-lapse imaging, we found that sensory dendrites form by...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2009.01.057
更新日期：2009-04-17 00:00:00
abstract：:The angiotensin II (AngII) type 1 receptor (AT1R) is a critical regulator of cardiovascular and renal function and is an important model for studies of G-protein-coupled receptor (GPCR) signaling. By stabilizing the receptor with a single-domain antibody fragment ("nanobody") discovered using a synthetic yeast-display...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2018.12.006
更新日期：2019-01-24 00:00:00
abstract：:The dynamic synapse is represented by the constant mobility and exchange of components at both the cell surface and at intracellular sites. This includes thermally powered Brownian diffusion movement, followed by reversible trapping through receptor-scaffold interactions and active transport of cargo vesicles through ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2014.06.002
更新日期：2014-06-19 00:00:00
abstract：:We describe here a bizarre organization of the single large subunit (LSU) and small subunit (SSU) rRNA genes in the mitochondrial DNA of Chlamydomonas reinhardtii. Each gene is discontinuous, with gene pieces encoding specific rRNA domains ("modules") interspersed with one another and with intact protein coding and tR...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(88)90026-8
更新日期：1988-11-04 00:00:00
abstract：:Maintaining the pluripotency of mouse ES cells requires both LIF (leukemia inhibitory factor) and unknown factors in serum. The paper from Ying et al. in this issue of Cell shows that BMP (bone morphogenetic protein) can replace serum in this capacity, defining molecular requirements for ES cell self-renewal. ...
journal_title：Cell
pub_type： 评论,杂志文章
doi：10.1016/s0092-8674(03)00853-5
更新日期：2003-10-31 00:00:00
abstract：:Activation of the adenylyl cyclase signaling pathway elicits the induction of genes via activators binding to cAMP-responsive elements (CREs). Nuclear factor CRE modulator (CREM) is activated by PKA-mediated phosphorylation on a serine at position 117. We show that Ser-117 is also phosphorylated by the mitogen-activat...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(94)90402-2
更新日期：1994-10-07 00:00:00
abstract：:Recent work has identified a cascade of membrane bound protein kinases in Ehrlich ascites tumor cells. These enzymes, designated PKL, PKS and PKM, are present in both Ehrlich tumor and mouse brain, but the cascade is active only in the tumor tissue. We have now purified a fourth protein kinase, PKF, that is also assoc...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(81)90227-0
更新日期：1981-07-01 00:00:00
