Abstract:
:Basal cells are by definition located on the basolateral side of several epithelia, and they have never been observed reaching the lumen. Using high-resolution 3D confocal imaging, we report that basal cells extend long and slender cytoplasmic projections that not only reach toward the lumen but can cross the tight junction barrier in some epithelia of the male reproductive and respiratory tracts. In this way, the basal cell plasma membrane is exposed to the luminal environment. In the epididymis, in which luminal acidification is crucial for sperm maturation and storage, these projections contain the angiotensin II type 2 receptor (AGTR2). Activation of AGTR2 by luminal angiotensin II, increases proton secretion by adjacent clear cells, which are devoid of AGTR2. We propose a paradigm in which basal cells scan and sense the luminal environment of pseudostratified epithelia and modulate epithelial function by a mechanism involving crosstalk with other epithelial cells.
journal_name
Celljournal_title
Cellauthors
Shum WW,Da Silva N,McKee M,Smith PJ,Brown D,Breton Sdoi
10.1016/j.cell.2008.10.020subject
Has Abstractpub_date
2008-12-12 00:00:00pages
1108-17issue
6eissn
0092-8674issn
1097-4172pii
S0092-8674(08)01312-3journal_volume
135pub_type
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