Abstract：
:Plasmids containing the origin region of human mtDNA are specifically transcribed by the partially purified homologous mtRNA polymerase in vitro. Transcription of both the light and heavy strands initiates at the same sites previously identified as in vivo transcription start sites. The sequences responsible for initiation of heavy strand transcription are investigated in detail, since this transcript includes the rRNA cistrons and the majority of tRNA genes and potential mRNAs. Deletion mutagenesis is employed to delimit the sequences responsible for heavy strand transcription to a region of less than 35 bp surrounding the transcription start site. This region contains a repetitive sequence, AAACCCC, and shows homology to other regions of the human mtDNA that have been implicated as transcription initiation sites or that show unusual homology to other mammalian mtDNA genomes.
journal_name
Celljournal_title
Cellauthors
Bogenhagen DF,Applegate EF,Yoza BKdoi
10.1016/0092-8674(84)90061-8subject
Has Abstractpub_date
1984-04-01 00:00:00pages
1105-13issue
4eissn
0092-8674issn
1097-4172pii
0092-8674(84)90061-8journal_volume
36pub_type
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