Abstract：
:A transglutaminase-catalyzed cross-linking process characteristic of keratinocytes leads to the formation of the insoluble corneocyte envelope. The essentials of this process take place in vitro in a reconstituted system derived from subcellular fractions. A particulate fraction containing membrane-bound envelope precursor proteins and the enzyme transglutaminase is combined with cytosolic proteins; when the enzyme is activated by Ca++, cytosolic proteins are removed from solution and cross-linked to particulate proteins. This interaction is cell-type-specific, since particulates derived from fibroblasts and also containing transglutaminase activity cannot substitute for those of keratinocytes. Involucrin, a cytosolic protein known to be a precursor of the envelope, is more efficiently cross-linked than other cytosolic proteins. The cross-linking of proteins of the particulate fraction (membrane proteins) is promoted by the presence of involucrin.
journal_name
Celljournal_title
Cellauthors
Simon M,Green Hdoi
10.1016/0092-8674(85)90216-8subject
Has Abstractpub_date
1985-03-01 00:00:00pages
677-83issue
3eissn
0092-8674issn
1097-4172pii
0092-8674(85)90216-8journal_volume
40pub_type
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