Abstract：
:Chemical cross-linking and DNA sequencing have revealed regions of intra-chromosomal interaction, referred to as topologically associating domains (TADs), interspersed with regions of little or no interaction, in interphase nuclei. We find that TADs and the regions between them correspond with the bands and interbands of polytene chromosomes of Drosophila. We further establish the conservation of TADs between polytene and diploid cells of Drosophila. From direct measurements on light micrographs of polytene chromosomes, we then deduce the states of chromatin folding in the diploid cell nucleus. Two states of folding, fully extended fibers containing regulatory regions and promoters, and fibers condensed up to 10-fold containing coding regions of active genes, constitute the euchromatin of the nuclear interior. Chromatin fibers condensed up to 30-fold, containing coding regions of inactive genes, represent the heterochromatin of the nuclear periphery. A convergence of molecular analysis with direct observation thus reveals the architecture of interphase chromosomes.
journal_name
Celljournal_title
Cellauthors
Eagen KP,Hartl TA,Kornberg RDdoi
10.1016/j.cell.2015.10.026subject
Has Abstractpub_date
2015-11-05 00:00:00pages
934-46issue
4eissn
0092-8674issn
1097-4172pii
S0092-8674(15)01340-9journal_volume
163pub_type
杂志文章相关文献
CELL文献大全abstract：:We have studied the interaction of highly purified Int protein with DNA restriction fragments from the lambda phage attachment site (attP) region. Two different DNA sequences are protected by bound Int protein against partial digestion by either pancreatic DNAase or neocarzinostatin. One Int binding site includes the ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(79)90049-7
更新日期：1979-10-01 00:00:00
abstract：:It is increasingly appreciated that oncogenic transformation alters cellular metabolism to facilitate cell proliferation, but less is known about the metabolic changes that promote cancer cell aggressiveness. Here, we analyzed metabolic gene expression in cancer cell lines and found that a set of high-grade carcinoma ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2014.07.032
更新日期：2014-08-28 00:00:00
abstract：:While the nuclear lamin proteins (A, B, and C) assemble specifically at the surface of the nuclear membrane, their sequences do not reveal stretches of hydrophobic amino acids that might explain their association with the nuclear membranes. However, the A and B lamin proteins possess Ras-like C-terminal CaaX sequence ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(89)90753-8
更新日期：1989-12-22 00:00:00
abstract：:The development of solid tumors is regulated by dynamic interactions between evolving neoplastic cells and their microenvironment. Luo et al. (2007) recently demonstrated that tumor-infiltrating immune cells expressing RANKL induce activation and nuclear localization of IKKalpha in prostatic epithelial tumor cells. Th...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2007.03.029
更新日期：2007-04-06 00:00:00
abstract：:Many developing and adult tissues are comprised of polarized epithelia. Proteins that are asymmetrically distributed in these cells are thought to be localized by protein trafficking. Here we show that the distribution and function of the signaling protein Wingless is predetermined by the subcellular localization of i...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(01)00311-7
更新日期：2001-04-20 00:00:00
abstract：:During membrane fusion, the outer leaflets of the two membranes merge first, whereas the distal membrane leaflets remain separate until the opening of a fusion pore. This intermediate stage, called hemifusion, is a critical event shared by exocytosis, protein trafficking, and viral entry. ...
journal_title：Cell
pub_type： 杂志文章,评审
doi：10.1016/j.cell.2005.10.015
更新日期：2005-11-04 00:00:00
abstract：:beta-glucocerebrosidase, the enzyme defective in Gaucher disease, is targeted to the lysosome independently of the mannose-6-phosphate receptor. Affinity-chromatography experiments revealed that the lysosomal integral membrane protein LIMP-2 is a specific binding partner of beta-glucocerebrosidase. This interaction in...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2007.10.018
更新日期：2007-11-16 00:00:00
abstract：:Affordable genome sequencing technologies promise to revolutionize the field of human genetics by enabling comprehensive studies that interrogate all classes of genome variation, genome-wide, across the entire allele frequency spectrum. Ongoing projects worldwide are sequencing many thousands-and soon millions-of huma...
journal_title：Cell
pub_type： 杂志文章,评审
doi：10.1016/j.cell.2019.02.032
更新日期：2019-03-21 00:00:00
abstract：:To initiate a genetic study of the hormonal regulation of genes coding for the three vitellogenins or yolk polypeptide precursors (YPs) in Drosophila, we have isolated from wild flies genetic variants which alter the mobility in SDS-PAGE of each YP independently. These variants are expressed codominantly and they are ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(80)90313-x
更新日期：1980-07-01 00:00:00
abstract：:TET-mediated 5-methyl cytosine (5mC) oxidation acts in epigenetic regulation, stem cell development, and cancer. Hu et al. now determine the crystal structure of the TET2 catalytic domain bound to DNA, shedding light on 5mC-DNA substrate recognition and the catalytic mechanism of 5mC oxidation. ...
journal_title：Cell
pub_type： 评论,杂志文章
doi：10.1016/j.cell.2013.12.002
更新日期：2013-12-19 00:00:00
abstract：:The precise control of miR-17∼92 microRNA (miRNA) is essential for normal development, and overexpression of certain miRNAs from this cluster is oncogenic. Here, we find that the relative expression of the six miRNAs processed from the primary (pri-miR-17∼92) transcript is dynamically regulated during embryonic stem c...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2015.07.008
更新日期：2015-08-13 00:00:00
abstract：:We have reconstituted the fusion and assembly of vesiculated Golgi membranes (VGMs) into functionally active stacks of cisternae. A kinetic analysis of this assembly process revealed that highly dispersed VGMs of 60-90 nm diameter first fuse to form larger vesicles of 200-300 nm diameter that are clustered together. T...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(95)90269-4
更新日期：1995-09-22 00:00:00
abstract：:Antigen presenting cells (macrophages and dendritic cells) express pattern recognition molecules that are thought to recognize foreign ligands during early phases of the immune response. The best known of these are probably the Toll-like receptors, but a number of other receptors are also involved. Several of these re...
journal_title：Cell
pub_type： 杂志文章,评审
doi：10.1016/s0092-8674(02)01201-1
更新日期：2002-12-27 00:00:00
abstract：:Transcriptional pausing by multisubunit RNA polymerases (RNAPs) is a key mechanism for regulating gene expression in both prokaryotes and eukaryotes and is a prerequisite for transcription termination. Pausing and termination states are thought to arise through a common, elemental pause state that is inhibitory for nu...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2012.12.020
更新日期：2013-01-31 00:00:00
abstract：:During eukaryotic translation initiation, initiator tRNA does not insert fully into the P decoding site on the 40S ribosomal subunit. This conformation (POUT) is compatible with scanning mRNA for the AUG start codon. Base pairing with AUG is thought to promote isomerization to a more stable conformation (PIN) that arr...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2014.10.001
更新日期：2014-10-23 00:00:00
abstract：:The initiation of chromosome segregation at anaphase is linked by the spindle assembly checkpoint to the completion of chromosome-microtubule attachment during metaphase. To determine the function of the mitotic checkpoint protein Mad2 during normal cell division and when mitosis goes awry, we have knocked out Mad2 in...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(00)80875-2
更新日期：2000-06-09 00:00:00
abstract：:Tumor suppressors block the development of cancer and are often lost during tumor development. Papa et al. show that partial loss of normal PTEN tumor suppressor function can be compounded by additional disruption caused by the expression of inactive mutant PTEN protein. This has significant implications for patients ...
journal_title：Cell
pub_type： 评论,杂志文章
doi：10.1016/j.cell.2014.04.008
更新日期：2014-04-24 00:00:00
abstract：:We have examined the molecular basis of the response of individuals of D. melanogaster to artificial selection for high and low abdominal bristles. By monitoring the fate of particular rDNA spacer length variants associated with individually isolated X and Y chromosomes, we show that flies from the low bristle number ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(83)90027-2
更新日期：1983-07-01 00:00:00
abstract：:During the process of chromatin diminution, which takes place in all presomatic cells of the early Ascaris embryo, the heterochromatic termini of the chromosomes are lost. Here we show that the newly formed ends of the reduced somatic chromosomes carry tandem repeats of the telomeric sequence TTAGGC. Comparison of a c...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(91)90076-b
更新日期：1991-11-15 00:00:00
abstract：:The Hippo-YAP pathway regulates organ size by modulating cell proliferation and apoptosis. Yu et al. now reveal that G-protein-coupled receptors act upstream of the transcriptional coactivators YAP/TAZ. This study reinforces the connection between the actin cytoskeleton and Hippo pathway activity and identifies a clas...
journal_title：Cell
pub_type： 评论,杂志文章
doi：10.1016/j.cell.2012.07.020
更新日期：2012-08-17 00:00:00
abstract：:It is established that tumor cell-derived VEGF acts on endothelial cells to promote angiogenesis and tumor growth. Here, we demonstrate that in K5-SOS-dependent mouse skin tumors, autocrine VEGF is required for tumor cell proliferation in a cell-autonomous and angiogenesis-independent manner. VEGF is upregulated in SO...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2009.12.046
更新日期：2010-01-22 00:00:00
abstract：:Females but not males of the low-lymphoma RF/J strain transmit a non-Mendelian factor which suppresses the development of lymphoma in F1 crosses with mice of the high-lymphoma AKR/J strain. Suppression of lymphoma was also evident in the first backcross generation to the parental AKR strain, but only when (RF female x...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(80)90517-6
更新日期：1980-02-01 00:00:00
abstract：:Mutations in the C. elegans gene ced-3 prevent almost all programmed cell deaths, so that in a ced-3 mutant there are many extra cells. We show that the pharyngeal neuron M4 is essential for feeding in wild-type worms, but in a ced-3 mutant, one of the extra cells, probably MSpaaaaap (the sister of M4), can sometimes ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(87)90593-9
更新日期：1987-12-24 00:00:00
abstract：:In contrast to uninfected chicken embryo fibroblasts (CEFs), CEFs infected with a retroviral vector that carries the v-erbA gene of avian erythroblastosis virus displayed new properties. These included limited anchorage-independent growth in soft agar, growth without latency in serum-supplemented medium, ability to ov...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(87)90545-9
更新日期：1987-06-05 00:00:00
abstract：:The potential of stem cells in regenerative medicine relies upon removing them from their natural habitat, propagating them in culture, and placing them into a foreign tissue environment. To do so, it is essential to understand how stem cells interact with their microenvironment, the so-called stem cell niche, to esta...
journal_title：Cell
pub_type： 杂志文章,评审
doi：10.1016/s0092-8674(04)00255-7
更新日期：2004-03-19 00:00:00
abstract：:Isolation of genes encoding the receptors for steroids, retinoids, vitamin D, and thyroid hormone and their structural and functional analysis revealed an evolutionarily conserved template for nuclear hormone receptors. This discovery sparked identification of numerous genes encoding related proteins, termed orphan re...
journal_title：Cell
pub_type： 杂志文章,评审
doi：10.1016/j.cell.2014.03.012
更新日期：2014-03-27 00:00:00
abstract：:The germinal center (GC) reaction produces high-affinity antibodies by random mutation and selective clonal expansion of B cells with high-affinity receptors. The mechanism by which B cells are selected remains unclear, as does the role of the two anatomically defined areas of the GC, light zone (LZ) and dark zone (DZ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2010.10.032
更新日期：2010-11-12 00:00:00
abstract：:The transcription factor HIF-1 mediates adaptive responses to hypoxia, and its activity is negatively regulated by O2-dependent binding of the von Hippel-Lindau (VHL) protein. In this issue, Boutin et al. (2008) use conditional knockout mice to demonstrate that sensing of O2 by keratinocytes in the epidermis leads to ...
journal_title：Cell
pub_type： 评论,杂志文章
doi：10.1016/j.cell.2008.04.004
更新日期：2008-04-18 00:00:00
abstract：:Iron is ubiquitous in the environment and in biology. The study of iron biology focuses on physiology and homeostasis-understanding how cells and organisms regulate their iron content, how diverse tissues orchestrate iron allocation, and how dysregulated iron homeostasis leads to common hematological, metabolic, and n...
journal_title：Cell
pub_type： 杂志文章,评审
doi：10.1016/s0092-8674(04)00343-5
更新日期：2004-04-30 00:00:00
abstract：:The budding yeast, Saccharomyces cerevisiae, has emerged as an archetype of eukaryotic cell biology. Here we show that S. cerevisiae is also a model for the evolution of cooperative behavior by revisiting flocculation, a self-adherence phenotype lacking in most laboratory strains. Expression of the gene FLO1 in the la...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2008.09.037
更新日期：2008-11-14 00:00:00
