Abstract：
:Nuclear pore complexes (NPCs) mediate transport across the nuclear envelope. In yeast, they also interact with active genes, attracting or retaining them at the nuclear periphery. In higher eukaryotes, some NPC components (nucleoporins) are also found in the nucleoplasm, with a so far unknown function. We have functionally characterized nucleoporin-chromatin interactions specifically at the NPC or within the nucleoplasm in Drosophila. We analyzed genomic interactions of full-length nucleoporins Nup98, Nup50, and Nup62 and nucleoplasmic and NPC-tethered forms of Nup98. We found that nucleoporins predominantly interacted with transcriptionally active genes inside the nucleoplasm, in particular those involved in developmental regulation and the cell cycle. A smaller set of nonactive genes interacted with the NPC. Genes strongly interacting with nucleoplasmic Nup98 were downregulated upon Nup98 depletion and activated on nucleoplasmic Nup98 overexpression. Thus, nucleoporins stimulate developmental and cell-cycle gene expression away from the NPC by interacting with these genes inside the nucleoplasm.
journal_name
Celljournal_title
Cellauthors
Kalverda B,Pickersgill H,Shloma VV,Fornerod Mdoi
10.1016/j.cell.2010.01.011subject
Has Abstractpub_date
2010-02-05 00:00:00pages
360-71issue
3eissn
0092-8674issn
1097-4172pii
S0092-8674(10)00012-7journal_volume
140pub_type
杂志文章相关文献
CELL文献大全abstract：:Activating mutations of FLT3 occur in about 30% of acute myeloid leukemia (AML) cases and are associated with relapse and poor prognosis. Midostaurin is the first drug approved for AML since 2000, and the first multi-kinase inhibitor approved for the FLT3-mutant subtype. To view this Bench to Bedside, open or download...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2017.11.007
更新日期：2017-11-16 00:00:00
abstract：:Hematopoietic stem cells (HSCs) develop from a specialized subpopulation of endothelial cells known as hemogenic endothelium (HE). Although the HE origin of HSCs is now well established in different species, the signaling pathways that control this transition remain poorly understood. Here, we show that activation of ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2013.08.055
更新日期：2013-09-26 00:00:00
abstract：:Release of CHO-K1 cells from plateau or stationary phase and reentry into the cell cycle is specifically and reversibly blocked at two distinct sites by the anticalmodulin drug W13. The first block occurs early during release while the cells are still at G0/G1, whereas the second occurs later in reentry during early S...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(84)90075-8
更新日期：1984-01-01 00:00:00
abstract：:The mechanisms of transcriptional activation in heterochromatin were investigated by using FISH to directly visualize changes in chromatin organization during activation of a heterochromatic lambda5 transgene. A DNase I hypersensitive site was shown to relocate the transgene to the outside of the pericentromeric heter...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(00)00177-x
更新日期：2000-11-22 00:00:00
abstract：:sigma(70) subunit is thought to be released from the core RNA polymerase (RNAP) upon the transition from initiation to elongation or shortly afterward. Here, we identify a population of RNAP from E. coli that retains sigma(70) throughout elongation. The relative amount of this population appears to depend on cellular ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(01)00461-5
更新日期：2001-08-24 00:00:00
abstract：:A key aspect of genomic medicine is to make individualized clinical decisions from personal genomes. We developed a machine-learning framework to integrate personal genomes and electronic health record (EHR) data and used this framework to study abdominal aortic aneurysm (AAA), a prevalent irreversible cardiovascular ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2018.07.021
更新日期：2018-09-06 00:00:00
abstract：:Human hepatitis B virus core protein expressed in E. coli assembles into two sizes of particle. We have determined their three-dimensional structures by electron cryomicroscopy and image processing. The large and small particles correspond to triangulation number T = 4 and T = 3 dimer clustered packings, containing 24...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(94)90142-2
更新日期：1994-06-17 00:00:00
abstract：:N(6)-methyladenosine (m(6)A) is the most abundant internal modification of messenger RNA. While the presence of m(6)A on transcripts can impact nuclear RNA fates, a reader of this mark that mediates processing of nuclear transcripts has not been identified. We find that the RNA-binding protein HNRNPA2B1 binds m(6)A-be...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2015.08.011
更新日期：2015-09-10 00:00:00
abstract：:How transcription is silenced in early embryos has long been a mystery. In this issue, Guven-Ozkan et al. (2008) report that transcriptional repression during worm embryogenesis is mediated through sequestration of the general transcription factor TAF-4 and is regulated by mechanisms that orchestrate the transition be...
journal_title：Cell
pub_type： 评论,杂志文章
doi：10.1016/j.cell.2008.09.021
更新日期：2008-10-03 00:00:00
abstract：:Dysfunction and loss of insulin-producing pancreatic beta cells represent hallmarks of diabetes mellitus. Here, we show that mice lacking the mitogen-activated protein kinase (MAPK) p38delta display improved glucose tolerance due to enhanced insulin secretion from pancreatic beta cells. Deletion of p38delta results in...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2008.11.018
更新日期：2009-01-23 00:00:00
abstract：:Growth factors can promote cell survival by activating the phosphatidylinositide-3'-OH kinase and its downstream target, the serine-threonine kinase Akt. However, the mechanism by which Akt functions to promote survival is not understood. We show that growth factor activation of the PI3'K/Akt signaling pathway culmina...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(00)80405-5
更新日期：1997-10-17 00:00:00
abstract：:The existence of pseudogenes and the observation of intron loss suggest that RNA can serve as an intermediate in recombination. We used a HIS3 reporter gene to show that RNA-mediated recombination occurs in yeast. His3+ prototroph formation required transcription and expression of the retrotransposon Ty. Two RNA-media...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(91)90187-4
更新日期：1991-10-18 00:00:00
abstract：:Rat liver interphase chromosomes have telomeres 20-100 kb in length. Micrococcal nuclease digestion of nuclei cleaves telomeres with a uniform 157 bp periodicity, producing soluble particles that sediment in sucrose gradients exactly like oligonucleosomes. The monomeric telomere particles comigrate with nucleosome cor...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(93)90256-p
更新日期：1993-05-21 00:00:00
abstract：:In the nucleus of eukaryotic cells, RNA decay in the 3'-5' direction is carried out by a complex of exonucleases called the exosome. Surprisingly, the purified exosome shows only weak activity in vitro. Two papers in this issue of Cell, by LaCava et al. (2005) and Wyers et al. (2005), and a third report by Vanacova et...
journal_title：Cell
pub_type： 评论,杂志文章
doi：10.1016/j.cell.2005.05.018
更新日期：2005-06-03 00:00:00
abstract：:In eukaryotic cells, organelles and the cytoskeleton undergo highly dynamic yet organized interactions capable of orchestrating complex cellular functions. Visualizing these interactions requires noninvasive, long-duration imaging of the intracellular environment at high spatiotemporal resolution and low background. T...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2018.09.057
更新日期：2018-11-15 00:00:00
abstract：:We have used cloned chicken cDNA sequences for alpha- and beta-tubulin to investigate tubulin gene organization in Drosophila melanogaster. Experiments using genomic Drosophila DNA from several sources indicate that there are at least four copies each of the alpha-tubulin gene and the beta-tubulin gene. In situ hybrid...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(80)90561-9
更新日期：1980-12-01 00:00:00
abstract：:During the process of chromatin diminution, which takes place in all presomatic cells of the early Ascaris embryo, the heterochromatic termini of the chromosomes are lost. Here we show that the newly formed ends of the reduced somatic chromosomes carry tandem repeats of the telomeric sequence TTAGGC. Comparison of a c...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(91)90076-b
更新日期：1991-11-15 00:00:00
abstract：:Invasive lobular carcinoma (ILC) is the second most prevalent histologic subtype of invasive breast cancer. Here, we comprehensively profiled 817 breast tumors, including 127 ILC, 490 ductal (IDC), and 88 mixed IDC/ILC. Besides E-cadherin loss, the best known ILC genetic hallmark, we identified mutations targeting PTE...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2015.09.033
更新日期：2015-10-08 00:00:00
abstract：:Cell surface complexes of class I MHC molecules and bound peptide antigens serve as specific recognition elements controlling the cytotoxic immune response. The 2.1 A structure of the human class I MHC molecule HLA-B27 provides a detailed composite image of a co-crystallized collection of HLA-B27-bound peptides, indic...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(92)90252-8
更新日期：1992-09-18 00:00:00
abstract：:The scrapie (PrPSc) and cellular (PrPC) prion proteins are encoded by the same gene, and their different properties are thought to arise from posttranslational modifications. We have found a phosphatidylinositol glycolipid on both PrPC and PrP 27-30 (derived from PrPSc by limited proteolysis at the amino terminus). Et...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(87)90150-4
更新日期：1987-10-23 00:00:00
abstract：:Several metabolic alterations accumulate over time along with a reduction in biological fitness, suggesting the existence of a "metabolic clock" that controls aging. Multiple inborn defects in metabolic circuitries accelerate aging, whereas genetic loci linked to exceptional longevity influence metabolism. Each of the...
journal_title：Cell
pub_type： 杂志文章,评审
doi：10.1016/j.cell.2016.07.031
更新日期：2016-08-11 00:00:00
abstract：:Recombinant E. coli that produce light were found in a clone library of hybrid plasmids containing DNA from the marine bacterium Vibrio fischeri. All luminescent clones had a 16 kb insert that encoded enzymatic activities for the light reaction as well as regulatory functions necessary for expression of the luminescen...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(83)90063-6
更新日期：1983-03-01 00:00:00
abstract：:In many organisms, meiotic chromosomes are bundled at their telomeres to form a "bouquet" arrangement. The bouquet formation plays an important role in homologous chromosome pairing and therefore progression of meiosis. As meiotic telomere clustering occurs in response to mating pheromone signaling in fission yeast, w...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2006.01.048
更新日期：2006-04-07 00:00:00
abstract：:We have identified two major and approximately ten minor poly(A)-containing RNA species in S. cerevisiae which arise from in vivo transcription of the yeast plasmid, known as 2mu circle. The two major species, which are 1325 and 1275 bases in length, are transcribed from the two unique halves of the plasmid and extend...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(79)90098-9
更新日期：1979-04-01 00:00:00
abstract：:Organ cultures of liver from untreated male Xenopus respond to 17 beta-estradiol in the culture medium by synthesizing and secreting the yolk protein precursor vitellogenin. Vitellogenin synthesis, as a primary response, is first detectable on the fourth day of culture, and comprises up to 12% of the protein synthesiz...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(76)90263-4
更新日期：1976-01-01 00:00:00
abstract：:Transfer of human chromosome 11, which contains the myoD locus, from primary fibroblasts into 10T1/2 cells results in activation of myoD. In contrast, hybrids that retain human chromosome 11 and additional human chromosomes fail to activate myoD. We show that human chromosome 4 inhibits myoD activation. myoD enhancer/...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(95)90033-0
更新日期：1995-08-25 00:00:00
abstract：:Molecular codes, like postal zip codes, are generally considered a robust way to ensure the specificity of neuronal target selection. However, a code capable of unambiguously generating complex neural circuits is difficult to conceive. Here, we re-examine the notion of molecular codes in the light of developmental alg...
journal_title：Cell
pub_type： 杂志文章,评审
doi：10.1016/j.cell.2015.09.031
更新日期：2015-10-08 00:00:00
abstract：:New biological tools provide new techniques to probe fundamental biological processes. Here we describe the burgeoning field of proteolysis-targeting chimeras (PROTACs), which are capable of modulating protein concentrations at a post-translational level by co-opting the ubiquitin-proteasome system. We describe the PR...
journal_title：Cell
pub_type： 杂志文章,评审
doi：10.1016/j.cell.2019.11.031
更新日期：2020-04-02 00:00:00
abstract：:RECQL5 is the sole member of the RECQ family of helicases associated with RNA polymerase II (RNAPII). We now show that RECQL5 is a general elongation factor that is important for preserving genome stability during transcription. Depletion or overexpression of RECQL5 results in corresponding shifts in the genome-wide R...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2014.03.048
更新日期：2014-05-22 00:00:00
abstract：:Nucleotide sequences in two wild-type and six mutant operators in the DNA of phage lambda are compared. Strikingly similar 17 base pair units are found which we identify as the repressor binding sites. Each operator contains multiple repressor binding sites separated by A-T rich spacers. Elements of 2 fold rotational ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(75)90018-5
更新日期：1975-06-01 00:00:00
