Abstract:
:Reversible S-palmitoylation of cysteine residues critically controls transient membrane tethering of peripheral membrane proteins. Little is known about how the palmitoylation machinery governs their defined localization and function. We monitored the spatially resolved reaction dynamics and substrate specificity of the core mammalian palmitoylation machinery using semisynthetic substrates. Palmitoylation is detectable only on the Golgi, whereas depalmitoylation occurs everywhere in the cell. The reactions are not stereoselective and lack any primary consensus sequence, demonstrating that substrate specificity is not essential for de-/repalmitoylation. Both palmitate attachment and removal require seconds to accomplish. This reaction topography and rapid kinetics allows the continuous redirection of mislocalized proteins via the post-Golgi sorting apparatus. Unidirectional secretion ensures the maintenance of a proper steady-state protein distribution between the Golgi and the plasma membrane, which are continuous with endosomes. This generic spatially organizing system differs from conventional receptor-mediated targeting mechanisms and efficiently counteracts entropy-driven redistribution of palmitoylated peripheral membrane proteins over all membranes.
journal_name
Celljournal_title
Cellauthors
Rocks O,Gerauer M,Vartak N,Koch S,Huang ZP,Pechlivanis M,Kuhlmann J,Brunsveld L,Chandra A,Ellinger B,Waldmann H,Bastiaens PIdoi
10.1016/j.cell.2010.04.007subject
Has Abstractpub_date
2010-04-30 00:00:00pages
458-71issue
3eissn
0092-8674issn
1097-4172pii
S0092-8674(10)00381-8journal_volume
141pub_type
杂志文章相关文献
CELL文献大全abstract::CRISPR-Cas9 is a versatile genome editing technology for studying the functions of genetic elements. To broadly enable the application of Cas9 in vivo, we established a Cre-dependent Cas9 knockin mouse. We demonstrated in vivo as well as ex vivo genome editing using adeno-associated virus (AAV)-, lentivirus-, or parti...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2014.09.014
更新日期:2014-10-09 00:00:00
abstract::Bacteria can respond to adverse environmental conditions by drastically reducing or even ceasing metabolic activity. They must then determine that conditions have improved before exiting dormancy, and one indication of such a change is the growth of other bacteria in the local environment. Growing bacteria release mur...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2008.08.039
更新日期:2008-10-31 00:00:00
abstract::A recombinant peptide encoding the CD11b A domain bound 54Mn2+ with a high affinity. Other divalent cations, including Mg2+, Zn2+, Ni2+, Co2+, and Cd2+, but not Ca2+ or Ba2+, competed effectively for Mn2+ binding. Amino acid substitutions within two conserved and noncontiguous regions in the recombinant peptide abolis...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(93)90575-b
更新日期:1993-03-26 00:00:00
abstract::Ste5 is a Zn2+ finger-like protein thought to function before three kinases, Ste11 (a MEKK), Ste7 (a MEK), and Fus3 (a MAPK), in a conserved MAP kinase cascade required for mating in S. cerevisiae. Here, we present evidence that Ste5 forms a multikinase complex that joins these kinases for efficient Fus3 activation. B...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(94)90427-8
更新日期:1994-08-12 00:00:00
abstract::TET-mediated 5-methyl cytosine (5mC) oxidation acts in epigenetic regulation, stem cell development, and cancer. Hu et al. now determine the crystal structure of the TET2 catalytic domain bound to DNA, shedding light on 5mC-DNA substrate recognition and the catalytic mechanism of 5mC oxidation. ...
journal_title:Cell
pub_type: 评论,杂志文章
doi:10.1016/j.cell.2013.12.002
更新日期:2013-12-19 00:00:00
abstract::The enteric bacteria Salmonella typhimurium has the ability to invade (enter) nonphagocytic cells. The internalization process occurs as a result of an intimate interaction between the bacteria and the host cell, in which S. typhimurium triggers a cascade of host cell-signaling events leading to the formation of host ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(94)90510-x
更新日期:1994-02-25 00:00:00
abstract::A complex interplay of environmental factors impacts the metabolism of human cells, but neither traditional culture media nor mouse plasma mimic the metabolite composition of human plasma. Here, we developed a culture medium with polar metabolite concentrations comparable to those of human plasma (human plasma-like me...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2017.03.023
更新日期:2017-04-06 00:00:00
abstract::During development, the precise relationships between transcription and chromatin modifications often remain unclear. We use the X chromosome inactivation (XCI) paradigm to explore the implication of chromatin changes in gene silencing. Using female mouse embryonic stem cells, we initiate XCI by inducing Xist and then...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2018.11.041
更新日期:2019-01-10 00:00:00
abstract::Characteristic of chronic myelogenous leukemia (CML) is the presence of the chimeric p210(bcr-abl) protein possessing elevated protein tyrosine kinase activity relative to normal c-abl tyrosine kinase. Hematopoietic progenitors isolated from CML patients in the chronic phase contain a constitutively tyrosine-phosphory...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(00)81840-1
更新日期:1997-01-24 00:00:00
abstract::Hybrid plasmid molecules containing tandemly repeated Drosophila satellite DNA were constructed using a modification of the (dA)-(dT) homopolymer procedure of Lobban and Kaiser (1973). Recombinant plasmids recovered after transformation of recA bacteria contained 10% of the amount of satellite DNA present in the trans...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(77)90038-1
更新日期:1977-03-01 00:00:00
abstract::While hematopoietic stem cell (HSC) self-renewal is well studied, it remains unknown whether distinct control mechanisms enable HSC divisions that generate progeny cells with specific lineage bias. Here, we report that the monocytic transcription factor MafB specifically restricts the ability of M-CSF to instruct myel...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2009.04.057
更新日期:2009-07-23 00:00:00
abstract::Subsets of CD4 T cells are defined by the cytokines that they produce; these cytokines determine the effector function of these cells. Cloned CD4 T cells fall into two subsets, producing either interferon-gamma (IFN gamma) or interleukin-4 (IL-4) in combination with other cytokines, and are called Th1 and Th2 cells, r...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(93)90542-x
更新日期:1993-12-03 00:00:00
abstract::Transfection experiments with HSV 1 in which one uses herpes simplex virus (HSV) thymidine kinase (TK) as a selectable prototrophic marker yield two classes of transformed cells: stable and unstable. In this report, we test the hypothesis that the stability phenotype can be explained by virus genome integration into a...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(78)90015-6
更新日期:1978-10-01 00:00:00
abstract::Mammalian cells express a spectrum of tubulin isotypes whose relationship to the diversity of microtubule function is unknown. To examine whether different isotypes are segregated into functionally distinct microtubules, we generated immune sera capable of discriminating among the various naturally occurring beta-tubu...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(87)90456-9
更新日期:1987-05-22 00:00:00
abstract::This year, the Lasker∼DeBakey Clinical Medical Research Award will be shared by Mahlon R. DeLong and Alim-Louis Benabid for elucidating the role of the subthalamic nucleus in mediating the motor dysfunction of Parkinson's disease and for pioneering the use of deep-brain stimulation to alleviate symptoms of the disease...
journal_title:Cell
pub_type: 传,历史文章,杂志文章
doi:10.1016/j.cell.2014.08.021
更新日期:2014-09-11 00:00:00
abstract::We have isolated cDNA clones encoding the four different forms of mouse myelin basic protein (MBP) and have analyzed the structure of the MBP gene. The three larger forms of MBP differ from the smallest by the inclusion of either or both of two short amino acid sequences at positions 57 and 124 of the smallest protein...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(85)90245-4
更新日期:1985-12-01 00:00:00
abstract::The mechanisms by which immune checkpoint blockade modulates tumor evolution during therapy are unclear. We assessed genomic changes in tumors from 68 patients with advanced melanoma, who progressed on ipilimumab or were ipilimumab-naive, before and after nivolumab initiation (CA209-038 study). Tumors were analyzed by...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2017.09.028
更新日期:2017-11-02 00:00:00
abstract::Telomerase activity was demonstrated in cell-free extracts from S. cerevisiae through the use of a PCR-based assay. As expected, this activity was eliminated by RNase or phenol treatment of the extract and was dependent on dGTP and dTTP. Telomerase was not detected in extracts prepared from cells grown for approximate...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(05)80017-0
更新日期:1995-06-30 00:00:00
abstract::The monoclonal antibody ECCD-1 inhibits Ca2+-dependent cell-cell adhesion in teratocarcinoma cells, recognizing a cell surface component of MW 124,000. When mouse embryos at various preimplantation stages were cultured in the presence of ECCD-1, the compacted morphology of the 8- to 16-cell-stage embryos was destroyed...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(83)90095-8
更新日期:1983-12-01 00:00:00
abstract::p120-catenin regulates cell-cell adhesion by controlling cell surface retention of cadherin. In this issue, Ishiyama et al. (2010) present the first crystal structure of p120 in complex with cadherin, revealing molecular details of the functional interface and providing sophisticated new tools for dissecting p120's ro...
journal_title:Cell
pub_type: 评论,杂志文章
doi:10.1016/j.cell.2010.03.022
更新日期:2010-04-02 00:00:00
abstract::In prokaryotes, DNA can be segregated by three different types of cytoskeletal filaments. The best-understood type of partitioning (par) locus encodes an actin homolog called ParM, which forms dynamically unstable filaments that push plasmids apart in a process reminiscent of mitosis. However, the most common type of ...
journal_title:Cell
pub_type: 杂志文章,评审
doi:10.1016/j.cell.2010.05.033
更新日期:2010-06-11 00:00:00
abstract::There are still no effective treatments to prevent, halt, or reverse Alzheimer's disease, but research advances over the past three decades could change this gloomy picture. Genetic studies demonstrate that the disease has multiple causes. Interdisciplinary approaches combining biochemistry, molecular and cell biology...
journal_title:Cell
pub_type: 杂志文章,评审
doi:10.1016/j.cell.2012.02.040
更新日期:2012-03-16 00:00:00
abstract::Phosphorylation of histone H3 serine 10 correlates with chromosome condensation and is required for normal chromosome segregation in Tetrahymena. This phosphorylation is dependent upon activation of the NIMA kinase in Aspergillus nidulans. NIMA expression also induces Ser-10 phosphorylation inappropriately in S phase-...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(00)00035-0
更新日期:2000-08-04 00:00:00
abstract::A new rhythm mutation was isolated based on its elimination of per-controlled luciferase cycling. Levels of period or timeless clock gene products in the mutant are flat in daily light-dark cycles or constant darkness (although PER and TIM oscillate normally in temperature cycles). Consistent with the fact that light ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(00)81638-4
更新日期:1998-11-25 00:00:00
abstract::GroEL and GroES form a chaperonin nano-cage for proteins up to approximately 60 kDa to fold in isolation. Here we explored the structural features of the chaperonin cage critical for rapid folding of encapsulated substrates. Modulating the volume of the GroEL central cavity affected folding speed in accordance with co...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2006.04.027
更新日期:2006-06-02 00:00:00
abstract::DDB1, a component of the Cul4 ubiquitin ligase complex, promotes protein ubiquitination in diverse cellular functions, including nuclear excision repair, regulation of the cell cycle, and DNA replication. To investigate its physiological significance, we generated mice with null and floxed alleles of the DDB1 gene. He...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2006.09.045
更新日期:2006-12-01 00:00:00
abstract::Ligand-directed signal bias offers opportunities for sculpting molecular events, with the promise of better, safer therapeutics. Critical to the exploitation of signal bias is an understanding of the molecular events coupling ligand binding to intracellular signaling. Activation of class B G protein-coupled receptors ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2016.05.023
更新日期:2016-06-16 00:00:00
abstract::We used the interaction trap, a yeast genetic selection for interacting proteins, to isolate human cyclin-dependent kinase interactor 1 (Cdi1). In yeast, Cdi1 interacts with cyclin-dependent kinases, including human Cdc2, Cdk2, and Cdk3, but not with Ckd4. In HeLa cells, Cdi1 is expressed at the G1 to S transition, an...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(93)90498-f
更新日期:1993-11-19 00:00:00
abstract::The Origin Recognition Complex (ORC) is a six-protein assembly that specifies the sites of DNA replication initiation in S. cerevisiae. Origin recognition by ORC requires ATP. Here, we demonstrate that two subunits, Orc1p and Orc5p, bind ATP and that Orc1p also hydrolyzes ATP. ATP binding and hydrolysis by Orc1p are b...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(00)81889-9
更新日期:1997-02-21 00:00:00
abstract::In S. cerevisiae, mitochondrial DNA (mtDNA) molecules, in spite of their high copy number, segregate as if there were a small number of heritable units. The rapid segregation of mitochondrial genomes can be analyzed using mtDNA deletion variants. These small, amplified genomes segregate preferentially from mixed zygot...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(95)90014-4
更新日期:1995-06-16 00:00:00