Structural features of the GroEL-GroES nano-cage required for rapid folding of encapsulated protein.

abstract：:Plasma membrane vesicles and protoplasts of Escherichia coli were fused to planar lipid bilayers and studied with electrophysiological techniques. Large transmembrane aqueous channels were opened when 0.2 nM LamB signal peptide was added to the cytoplasmic side of the membrane. These aqueous pores are similar in condu...

journal_title：Cell

authors： Simon SM,Blobel G

更新日期：1992-05-15 00:00:00

abstract：:Transfer RNA (tRNA) gene content is a differentiating feature of genomes that contributes to the efficiency of the translational apparatus, but the principles shaping tRNA gene copy number and codon composition are poorly understood. Here, we report that the emergence of two specific tRNA modifications shaped the stru...

journal_title：Cell

authors： Novoa EM,Pavon-Eternod M,Pan T,Ribas de Pouplana L

更新日期：2012-03-30 00:00:00

abstract：:Transduction of cellular genes by retroviruses requires two recombinations: one to form the left-hand junction between cellular gene and viral genome, the other to form the right-hand junction. Previous findings raised the possibility that the right-hand recombination might use RNA molecules as intermediates. We now p...

journal_title：Cell

authors： Huang CC,Hay N,Bishop JM

更新日期：1986-03-28 00:00:00

abstract：:N(6)-methyladenosine (m(6)A) is the most abundant internal modification in mammalian mRNA. This modification is reversible and non-stoichiometric and adds another layer to the dynamic control of mRNA metabolism. The stability of m(6)A-modified mRNA is regulated by an m(6)A reader protein, human YTHDF2, which recognize...

journal_title：Cell

authors： Wang X,Zhao BS,Roundtree IA,Lu Z,Han D,Ma H,Weng X,Chen K,Shi H,He C

更新日期：2015-06-04 00:00:00

abstract：:To understand the role of FoxO family members in hematopoiesis, we conditionally deleted FoxO1, FoxO3, and FoxO4 in the adult hematopoietic system. FoxO-deficient mice exhibited myeloid lineage expansion, lymphoid developmental abnormalities, and a marked decrease of the lineage-negative Sca-1+, c-Kit+ (LSK) compartme...

journal_title：Cell

authors： Tothova Z,Kollipara R,Huntly BJ,Lee BH,Castrillon DH,Cullen DE,McDowell EP,Lazo-Kallanian S,Williams IR,Sears C,Armstrong SA,Passegué E,DePinho RA,Gilliland DG

更新日期：2007-01-26 00:00:00

abstract：:Oligodendrocyte-specific protein (OSP)/claudin-11 is a recently identified transmembrane protein found in CNS myelin and testis with unknown function. Herein we demonstrate that Osp null mice exhibit both neurological and reproductive deficits: CNS nerve conduction is slowed, hindlimb weakness is conspicuous, and male...

journal_title：Cell

authors： Gow A,Southwood CM,Li JS,Pariali M,Riordan GP,Brodie SE,Danias J,Bronstein JM,Kachar B,Lazzarini RA

更新日期：1999-12-10 00:00:00

abstract：:We describe the identification and characterization of the Saccharomyces cerevisiae ZIP2 gene, which encodes a novel meiosis-specific protein essential for synaptonemal complex formation. In the zip2 mutant, chromosomes are homologously paired but not synapsed. The Zip2 protein localizes to discrete foci on meiotic ch...

journal_title：Cell

authors： Chua PR,Roeder GS

更新日期：1998-05-01 00:00:00

abstract：:Cellular ras activity has been neutralized in 3T3 cells by microinjection of a specific anti-ras monoclonal antibody. The injected antibody efficiently inhibited proliferation in cells treated with a phorbol ester and a calcium ionophore, or with prostaglandin F2 alpha. These treatments were designed to imitate the ac...

journal_title：Cell

authors： Yu CL,Tsai MH,Stacey DW

更新日期：1988-01-15 00:00:00

abstract：:The BALC/c mouse has two nonallelic beta-globin genes that appear to reside on two different Eco R1 fragments of genomic DNA. We have already cloned one of these fragments and shown that the gene encoded within it is interrupted by at least one large intervening sequence of DNA. We have now cloned and characterized th...

journal_title：Cell

authors： Tiemeier DC,Tilghman SM,Polsky FI,Seidman JG,Leder A,Edgell MH,Leder P

更新日期：1978-06-01 00:00:00

abstract：:Nucleosomes positioned over promoters are usually inhibitory to protein binding and activity. We analyzed at the nucleotide level of resolution the nucleosomal organization of a distal, liver-specific enhancer in various mouse tissues and found that the enhancer exists in an array of three precisely positioned nucleos...

journal_title：Cell

authors： McPherson CE,Shim EY,Friedman DS,Zaret KS

更新日期：1993-10-22 00:00:00

abstract：:The Bcl-2 family member Bax translocates from the cytosol to mitochondria, where it oligomerizes and permeabilizes the mitochondrial outer membrane to promote apoptosis. Bax activity is counteracted by prosurvival Bcl-2 proteins, but how they inhibit Bax remains controversial because they neither colocalize nor form s...

journal_title：Cell

authors： Edlich F,Banerjee S,Suzuki M,Cleland MM,Arnoult D,Wang C,Neutzner A,Tjandra N,Youle RJ

更新日期：2011-04-01 00:00:00

abstract：:Planar polarity is a global, tissue-level phenomenon that coordinates cell behavior in a two-dimensional plane. The Frizzled/planar cell polarity (PCP) and anterior-posterior (AP) patterning systems for planar polarity operate in a variety of cell types and provide direction to cells with different morphologies and be...

journal_title：Cell

authors： Zallen JA

更新日期：2007-06-15 00:00:00

abstract：:We have cloned a Dictyostelium phosphotyrosine phosphatase (PTP1) with a catalytic domain showing approximately 38%-50% amino acid identity to those of other PTPs. PTP1 contains an approximately 99 amino acid insert and bacterially produced PTP1 possesses PTP activity. PTP1 is expressed at a very low level in vegetati...

journal_title：Cell

authors： Howard PK,Sefton BM,Firtel RA

更新日期：1992-11-13 00:00:00

abstract：:We have purified and characterized a modified peptide pheromone that accumulates in culture medium as B. subtilis grows to high density. This pheromone is required for the development of genetic competence. When added to cells at low density, the pheromone induces the premature development of competence. The peptide m...

journal_title：Cell

authors： Magnuson R,Solomon J,Grossman AD

更新日期：1994-04-22 00:00:00

abstract：:Induction of pluripotency in somatic cells has been achieved by myriad combinations of transcription factors that belong to the core pluripotency circuitry. In this issue, Shu et al. report reprogramming with lineage specifiers, lending support to the view of the pluripotent state as a fine balance between competing d...

journal_title：Cell

authors： Ben-David U,Nissenbaum J,Benvenisty N

更新日期：2013-05-23 00:00:00

abstract：:Following DNA replication, eukaryotic cells must biorient all sister chromatids prior to cohesion cleavage at anaphase. In animal cells, sister chromatids gradually biorient during prometaphase, but current models of mitosis in S. cerevisiae assume that biorientation is established shortly after S phase. This assumpti...

journal_title：Cell

authors： Marco E,Dorn JF,Hsu PH,Jaqaman K,Sorger PK,Danuser G

更新日期：2013-08-29 00:00:00

abstract：:Morphogen gradients are typically analyzed from static images of fixed embryonic tissues. Two papers in this issue of Cell now report live imaging of the Bicoid gradient in developing fruit fly embryos (Gregor et al., 2007a, 2007b). Their findings indicate that the gradient is highly reproducible from embryo to embryo...

journal_title：Cell

authors： Gibson MC

更新日期：2007-07-13 00:00:00

abstract：:The single, chloroplast encoded gene for the large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase (rbcL) from Euglena gracilis is found to contain nine intervening sequences. The intervening sequences were identified by heteroduplex analysis between Euglena rbcL and the non-intron-containing rbcL from Spin...

journal_title：Cell

authors： Koller B,Gingrich JC,Stiegler GL,Farley MA,Delius H,Hallick RB

更新日期：1984-02-01 00:00:00

abstract：:Infections disturb metabolic homeostasis in many contexts, but the underlying connections are not completely understood. To address this, we use paired genetic and computational screens in Drosophila to identify transcriptional regulators of immunity and pathology and their associated target genes and physiologies. We...

journal_title：Cell

authors： Clark RI,Tan SW,Péan CB,Roostalu U,Vivancos V,Bronda K,Pilátová M,Fu J,Walker DW,Berdeaux R,Geissmann F,Dionne MS

更新日期：2013-10-10 00:00:00

abstract：:Autophagy is a cell-autonomous, catabolic process that plays context-dependent roles in tumor growth and progression. Wei et al. report that EGFR signaling promotes tumor growth through phosphorylation and functional inactivation of Beclin 1 and the consequent suppression of autophagy. ...

journal_title：Cell

authors： Fantin VR,Abraham RT

更新日期：2013-09-12 00:00:00

abstract：:Phagosomes were traditionally thought to originate from an invagination and scission of the plasma membrane to form a distinct intracellular vacuole. An alternative model implicating the endoplasmic reticulum (ER) as a major component of nascent and maturing phagosomes was recently proposed (Gagnon et al., 2002). To r...

journal_title：Cell

authors： Touret N,Paroutis P,Terebiznik M,Harrison RE,Trombetta S,Pypaert M,Chow A,Jiang A,Shaw J,Yip C,Moore HP,van der Wel N,Houben D,Peters PJ,de Chastellier C,Mellman I,Grinstein S

更新日期：2005-10-07 00:00:00

abstract：:Iron is ubiquitous in the environment and in biology. The study of iron biology focuses on physiology and homeostasis-understanding how cells and organisms regulate their iron content, how diverse tissues orchestrate iron allocation, and how dysregulated iron homeostasis leads to common hematological, metabolic, and n...

journal_title：Cell

authors： Hentze MW,Muckenthaler MU,Andrews NC

更新日期：2004-04-30 00:00:00

abstract：:The differentiation of follicular dendritic cells (FDC) is essential to the remarkable microanatomic plasticity of lymphoid follicles. Here we show that FDC arise from ubiquitous perivascular precursors (preFDC) expressing platelet-derived growth factor receptor β (PDGFRβ). PDGFRβ-Cre-driven reporter gene recombinatio...

journal_title：Cell

authors： Krautler NJ,Kana V,Kranich J,Tian Y,Perera D,Lemm D,Schwarz P,Armulik A,Browning JL,Tallquist M,Buch T,Oliveira-Martins JB,Zhu C,Hermann M,Wagner U,Brink R,Heikenwalder M,Aguzzi A

更新日期：2012-07-06 00:00:00

abstract：:Three-dimensional structures of a native simian and reassortant rotavirus have been determined by electron cryomicroscopy and computer image processing. The structural features of the native virus confirm that the hemagglutinin spike is a dimer of VP4, substantiated by in vivo radiolabeling studies. Exchange of native...

journal_title：Cell

authors： Shaw AL,Rothnagel R,Chen D,Ramig RF,Chiu W,Prasad BV

更新日期：1993-08-27 00:00:00

abstract：:The ubiquitin-specific processing protease (UBP) family of deubiquitinating enzymes plays an essential role in numerous cellular processes. HAUSP, a representative UBP, specifically deubiquitinates and hence stabilizes the tumor suppressor protein p53. Here, we report the crystal structures of the 40 kDa catalytic cor...

journal_title：Cell

authors： Hu M,Li P,Li M,Li W,Yao T,Wu JW,Gu W,Cohen RE,Shi Y

更新日期：2002-12-27 00:00:00

abstract：:Vanadate has been shown to inhibit phosphatases that remove phosphate groups from phosphotyrosine in cell-free systems. Addition of vanadate to the culture medium of NRK-1 cells resulted in a maximal 40-fold increase in the level of phosphotyrosine in cell protein. Also, vanadate induced transformation as evidenced by...

journal_title：Cell

authors： Klarlund JK

更新日期：1985-07-01 00:00:00

abstract：:The Drosha-DGCR8 complex, also known as Microprocessor, is essential for microRNA (miRNA) maturation. Drosha functions as the catalytic subunit, while DGCR8 (also known as Pasha) recognizes the RNA substrate. Although the action mechanism of this complex has been intensively studied, it remains unclear how Drosha and ...

journal_title：Cell

authors： Han J,Pedersen JS,Kwon SC,Belair CD,Kim YK,Yeom KH,Yang WY,Haussler D,Blelloch R,Kim VN

更新日期：2009-01-09 00:00:00

abstract：:The initiation of transcription from the nitrogen-regulated promoter glnAp2 requires RNA polymerase containing sigma 54, the transcriptional activator NRI, and the protein kinase NRII, responsible for the conversion of NRI to the active NRI-phosphate. NRI-phosphate does not increase the ability of sigma 54-containing ...

journal_title：Cell

authors： Ninfa AJ,Reitzer LJ,Magasanik B

更新日期：1987-09-25 00:00:00

abstract：:Cell-penetrating peptides (CPPs) are short protein segments that can transport cargos into cells. Although CPPs are widely studied as potential drug delivery tools, their role in normal cell physiology is poorly understood. Early during infection, the L2 capsid protein of human papillomaviruses binds retromer, a cytop...

journal_title：Cell

authors： Zhang P,Monteiro da Silva G,Deatherage C,Burd C,DiMaio D

更新日期：2018-09-06 00:00:00

abstract：:Hematopoietic stem cells (HSCs) develop from a specialized subpopulation of endothelial cells known as hemogenic endothelium (HE). Although the HE origin of HSCs is now well established in different species, the signaling pathways that control this transition remain poorly understood. Here, we show that activation of ...

journal_title：Cell

authors： Chanda B,Ditadi A,Iscove NN,Keller G

更新日期：2013-09-26 00:00:00