Abstract：
:Two allelic forms of the natural chicken ovalbumin gene have been independently cloned. These alleles differ from each other by an Eco RI restriction cleavage site in one of the seven intervening sequences within the natural ovalbumin gene. Restriction endonuclease mapping and sequence analyses of these cloned genotypic alleles have shown identical sequence organization and molecular structures of the interspersed structural and intervening sequences except for the particular Eco RI cleavage site. Sequencing data of the cloned DNA suggest that this Eco RI site may be created or eliminated by a single base mutation in the intervening sequence of the ovalbumin gene. The occurrence of apparent homozygous and heterozygous allelic forms of the ovalbumin gene in individual hens and roosters within the same breed has been observed. 10 and 40% of the chickens examined are homozygous for the ovalbumin gene with and without the extra Eco RI site, respectively, while 50% of them are heterozygous. Further analysis of individual chicken DNA cleaved by restriction endonuclease Hae III has revealed that there may be a series of such mutational variations within the ovalbumin gene. We have identified two Hae III cleavage sites that do not occur in all of the chickens, thus giving rise to several additional allelic variations of the ovalbumin gene. At least one of these Hae III sites is situated in the intervening sequence of the ovalbumin gene, and its lcoation has been mapped. Such allelic variations must be taken into consideration when determining eucaryotic gene structure by restriction mapping of the genomic DNA. Furthermore, this type of mutation within the intervening sequences of an eucaryotic gene has no known phenotypic manifestation. It represents an extrastructural silent mutation that must be taken account of in studies to estimate the rates of eucaryotic gene sequence divergence during evolution.
journal_name
Celljournal_title
Cellauthors
Lai EC,Woo SL,Dugaiczyk A,O'Malley BWdoi
10.1016/0092-8674(79)90201-0subject
Has Abstractpub_date
1979-01-01 00:00:00pages
201-11issue
1eissn
0092-8674issn
1097-4172pii
0092-8674(79)90201-0journal_volume
16pub_type
杂志文章相关文献
CELL文献大全abstract：:The MAVS protein plays a critical role in the assembly of an antiviral signaling complex on mitochondrial membranes. Hou et al. (2011) now report that virus infection induces a conformational change in MAVS, leading to the prion-like formation of functional self-aggregates that provide a sensitive trigger for antivira...
journal_title：Cell
pub_type： 评论,杂志文章
doi：10.1016/j.cell.2011.07.018
更新日期：2011-08-05 00:00:00
abstract：:We have identified and analyzed a meiotic reciprocal recombination hot spot in S. cerevisiae. We find that double-strand breaks occur at two specific sites associated with the hot spot and that occurrence of these breaks depends upon meiotic recombination functions RAD50 and SPO11. Furthermore, these breaks occur in a...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(90)90072-m
更新日期：1990-06-15 00:00:00
abstract：:A mechanism by which the nerve growth factor (NGF) signal is transduced to the nucleus to induce gene expression has been characterized. An NGF-inducible, Ras-dependent protein kinase has been identified that catalyzes the phosphorylation of the cyclic AMP response element-binding protein (CREB) at Ser-133. Phosphoryl...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(94)90055-8
更新日期：1994-06-03 00:00:00
abstract：:In Saccharomyces cerevisiae, mothers and daughters have distinct fates. We show that Cbk1 kinase and its interacting protein Mob2 regulate this asymmetry by inducing daughter-specific genetic programs. Daughter-specific expression is due to Cbk1/Mob2-dependent activation and localization of the Ace2 transcription fact...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(01)00596-7
更新日期：2001-12-14 00:00:00
abstract：:The tumor suppressor p53 exerts antiproliferation effects through its ability to function as a sequence-specific DNA-binding transcription factor. Here, we demonstrate that p53 can be modified by acetylation both in vivo and in vitro. Remarkably, the site of p53 that is acetylated by its coactivator, p300, resides in ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(00)80521-8
更新日期：1997-08-22 00:00:00
abstract：:E2F is a transcription factor believed to play a role in the activation of genes required for cellular proliferation. Its regulation is likely important for maintenance of G0 and for the initiation of cell growth. The retinoblastoma product (RB) forms a complex with E2F in G1 in primary and established human cells. As...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(92)90214-w
更新日期：1992-01-10 00:00:00
abstract：:Pseudomonas exotoxin (PE) was used as a probe to study the mechanism by which protein ligands are internalized by mammalian cells. Both biochemical and electron microscopic methods were used to look at the internalization of PE by mouse LM cell fibroblasts. Our data suggest that PE enters cells by receptor-mediated en...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(80)90450-x
更新日期：1980-10-01 00:00:00
abstract：:The large tumor antigen (LTag) of simian virus 40, an AAA(+) protein, is a hexameric helicase essential for viral DNA replication in eukaryotic cells. LTag functions as an efficient molecular machine powered by ATP binding and hydrolysis for origin DNA melting and replication fork unwinding. To understand how ATP bind...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2004.09.017
更新日期：2004-10-01 00:00:00
abstract：:We report the identification and characterization of a class of IS10 transposase mutants that carry out only some of the steps required for transposition. These mutants were identified among transposition-defective mutants as a specific subclass that retains the wild-type ability to induce SOS functions in the presenc...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(89)90299-7
更新日期：1989-10-20 00:00:00
abstract：:In the SV40-transformed mouse embryo fibroblast cell line SVT2/S, genomic rearrangements involving the SV40 DNA and flanking host sequences were identified by Southern blot hybridization using viral DNA as probe. No rearrangements of SV40 DNA integrated into nonpermissive mouse cells have been previously described. Th...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(81)90268-3
更新日期：1981-01-01 00:00:00
abstract：:Fibrillar protein aggregates are the major pathological hallmark of several incurable, age-related, neurodegenerative disorders. These aggregates typically contain aggregation-prone pathogenic proteins, such as amyloid-beta in Alzheimer's disease and alpha-synuclein in Parkinson's disease. It is, however, poorly under...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2010.07.020
更新日期：2010-08-20 00:00:00
abstract：:Mucus production by goblet cells of the large intestine serves as a crucial antimicrobial protective mechanism at the interface between the eukaryotic and prokaryotic cells of the mammalian intestinal ecosystem. However, the regulatory pathways involved in goblet cell-induced mucus secretion remain largely unknown. He...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2014.01.026
更新日期：2014-02-27 00:00:00
abstract：:Motile cells extend a leading edge by assembling a branched network of actin filaments that produces physical force as the polymers grow beneath the plasma membrane. A core set of proteins including actin, Arp2/3 complex, profilin, capping protein, and ADF/cofilin can reconstitute the process in vitro, and mathematica...
journal_title：Cell
pub_type： 杂志文章,评审
doi：10.1016/s0092-8674(03)00120-x
更新日期：2003-02-21 00:00:00
abstract：:Long-term severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) shedding was observed from the upper respiratory tract of a female immunocompromised individual with chronic lymphocytic leukemia and acquired hypogammaglobulinemia. Shedding of infectious SARS-CoV-2 was observed up to 70 days, and of genomic and s...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2020.10.049
更新日期：2020-12-23 00:00:00
abstract：:A clonally diverse anticipatory repertoire in which each lymphocyte bears a unique antigen receptor is the central feature of the adaptive immune system that evolved in our vertebrate ancestors. The survival advantage gained through adding this type of adaptive immune system to a pre-existing innate immune system led ...
journal_title：Cell
pub_type： 杂志文章,评审
doi：10.1016/j.cell.2006.02.001
更新日期：2006-02-24 00:00:00
abstract：:The v-erbB gene product of avian erythroblastosis virus (AEV) has extensive homology with the receptor for epidermal growth factor (EGF). We report here that chicken embryo fibroblasts (CEF) transformed by AEV show enhanced tyrosine phosphorylation of a number of cellular polypeptides, including the 36 kd protein, whi...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(85)90209-0
更新日期：1985-03-01 00:00:00
abstract：:A recombinant peptide encoding the CD11b A domain bound 54Mn2+ with a high affinity. Other divalent cations, including Mg2+, Zn2+, Ni2+, Co2+, and Cd2+, but not Ca2+ or Ba2+, competed effectively for Mn2+ binding. Amino acid substitutions within two conserved and noncontiguous regions in the recombinant peptide abolis...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(93)90575-b
更新日期：1993-03-26 00:00:00
abstract：:Pulses of ecdysteroids direct Drosophila through its life cycle by activating stage- and tissue-specific genetic regulatory hierarchies. Here we show that an orphan nuclear receptor, DHR78, functions at the top of the ecdysteroid regulatory hierarchies. Null mutations in DHR78 lead to lethality during the third larval...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(00)81184-8
更新日期：1998-05-15 00:00:00
abstract：:In April this year, a new influenza virus of swine origin emerged in Mexico and spread rapidly around the world. As the Northern hemisphere winter flu season kicks off, Laura Vargas-Parada reports on the measures that Mexico is taking to combat the H1N1 pandemic. ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2009.12.019
更新日期：2009-12-24 00:00:00
abstract：:Metabolic conditions affect the developmental tempo of animals. Developmental gene regulatory networks (GRNs) must therefore synchronize their dynamics with a variable timescale. We find that layered repression of genes couples GRN output with variable metabolism. When repressors of transcription or mRNA and protein s...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2019.06.023
更新日期：2019-08-08 00:00:00
abstract：:Phosphodiesterases (PDEs) regulate the local concentration of 3',5' cyclic adenosine monophosphate (cAMP) within cells. cAMP activates the cAMP-dependent protein kinase (PKA). In patients, PDE inhibitors have been linked to heart failure and cardiac arrhythmias, although the mechanisms are not understood. We show that...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2005.07.030
更新日期：2005-10-07 00:00:00
abstract：:Acquisition of the ability to produce and respond to a growth factor may result in increased cellular proliferation and could lead to malignant transformation. The fact that a large variety of tumor cells secrete transforming growth factor-alpha (TGF-alpha) suggests involvement of TGF-alpha in cellular transformation ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(86)90747-6
更新日期：1986-07-18 00:00:00
abstract：:Fragile X syndrome results from the expansion of a CGG repeat that is normally interrupted by occasional AGG triplets. Linkage disequilibrium suggests that certain normal haplotypes may contribute unequally to the pool of fragile X chromosomes. Sequence analysis of normal alleles demonstrates haplotype-specific variat...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(94)90134-1
更新日期：1994-06-17 00:00:00
abstract：:The discovery that somatic cells can be reprogrammed into induced pluripotent stem cells (iPSCs) raised the exciting possibility of modeling diseases with patient-specific cells. Marchetto et al. (2010) now use iPSC technology to generate, characterize, and treat an in vitro model for the autism spectrum disorder Rett...
journal_title：Cell
pub_type： 评论,杂志文章
doi：10.1016/j.cell.2010.10.037
更新日期：2010-11-12 00:00:00
abstract：:ABCA1, an ATP-binding cassette (ABC) subfamily A exporter, mediates the cellular efflux of phospholipids and cholesterol to the extracellular acceptor apolipoprotein A-I (apoA-I) for generation of nascent high-density lipoprotein (HDL). Mutations of human ABCA1 are associated with Tangier disease and familial HDL defi...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2017.05.020
更新日期：2017-06-15 00:00:00
abstract：:In a 4-cell stage C. elegans embryo, signaling by the P2 blastomere induces anterior-posterior polarity in the adjacent EMS blastomere, leading to endoderm formation. We have taken genetic and reverse genetic approaches toward understanding the molecular basis for this induction. These studies have identified a set of...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(00)80531-0
更新日期：1997-08-22 00:00:00
abstract：:In this study, we have located the sites of transcription initiation and termination on a cloned fragment of ribosomal DNA from X. laevis, and have sequenced the surrounding nucleotides. As reported previously (Reeder, Sollner-Webb and Wahn, 1977), about 25% of the 40S rRNA precursor molecules isolated from oocytes ha...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(79)90066-7
更新日期：1979-10-01 00:00:00
abstract：:The fovea is a specialized region of the retina that dominates the visual perception of primates by providing high chromatic and spatial acuity. While the foveal and peripheral retina share a similar core circuit architecture, they exhibit profound functional differences whose mechanisms are unknown. Using intracellul...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2017.01.005
更新日期：2017-01-26 00:00:00
abstract：:ATP-dependent proteases are vital to maintain cellular protein homeostasis. Here, we study the mechanisms of force generation and intersubunit coordination in the ClpXP protease from E. coli to understand how these machines couple ATP hydrolysis to mechanical protein unfolding. Single-molecule analyses reveal that pho...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2013.09.022
更新日期：2013-10-24 00:00:00
abstract：:Certain tRNAs in S. cerevisiae (tRNATyr and tRNAPhe) arise via precursor molecules which are mature at the 5' and 3' termini but contain intervening sequences adjacent to the anticodon (Knapp et al., 1978; O'Farrell et al., 1978). In addition to these molecules, precursors to several other tRNAs accumulate in a temper...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(79)90349-0
更新日期：1979-09-01 00:00:00
