Abstract:
:We have identified and analyzed a meiotic reciprocal recombination hot spot in S. cerevisiae. We find that double-strand breaks occur at two specific sites associated with the hot spot and that occurrence of these breaks depends upon meiotic recombination functions RAD50 and SPO11. Furthermore, these breaks occur in a processed form in wild-type cells and in a discrete, unprocessed form in certain nonnull rad50 mutants, rad50S, which block meiotic prophase at an intermediate stage. The breaks observed in wild-type cells are similar to those identified independently at another recombination hot spot, ARG4. We show here that the breaks at ARG4 also occur in discrete form in rad50S mutants. Occurrence of breaks in rad50S mutants is also dependent upon SPO11 function. These observations provide additional evidence that double-strand breaks are a prominent feature of meiotic recombination in yeast. More importantly, these observations begin to define a pathway for the physical changes in DNA that lead to recombination and to define the roles of meiotic recombination functions in that pathway.
journal_name
Celljournal_title
Cellauthors
Cao L,Alani E,Kleckner Ndoi
10.1016/0092-8674(90)90072-msubject
Has Abstractpub_date
1990-06-15 00:00:00pages
1089-101issue
6eissn
0092-8674issn
1097-4172pii
0092-8674(90)90072-Mjournal_volume
61pub_type
杂志文章相关文献
CELL文献大全abstract::The pandemic has impacted every scientist differently. Many negative impacts are frequently discussed. Here we highlight unexpected positives that we have found and hope will persist: improved access to experts; deeper and broader human engagement among colleagues, collaborators, and competitors; and significant democ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2020.09.027
更新日期:2020-10-01 00:00:00
abstract::Ligand-directed signal bias offers opportunities for sculpting molecular events, with the promise of better, safer therapeutics. Critical to the exploitation of signal bias is an understanding of the molecular events coupling ligand binding to intracellular signaling. Activation of class B G protein-coupled receptors ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2016.05.023
更新日期:2016-06-16 00:00:00
abstract::5, 6-dichloro-1-beta-D-ribofuranosylbenzimidazole (DRB; 75 muM) inhibited only 60-75% of nuclear heterogeneous RNA (hnRNA) synthesis in HeLa cells, while the appearance of labeled cytoplasmic poly(A)-containing messenger RNA (mRNA) was reduced by approximately 95%. DRB-sensitive and resistant transcripts spanned the e...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(76)90092-1
更新日期:1976-11-01 00:00:00
abstract::Many neural progenitors, including Drosophila mushroom body (MB) and projection neuron (PN) neuroblasts, sequentially give rise to different subtypes of neurons throughout development. We identified a novel BTB-zinc finger protein, named Chinmo (Chronologically inappropriate morphogenesis), that governs neuronal tempo...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2006.08.045
更新日期:2006-10-20 00:00:00
abstract::Infections disturb metabolic homeostasis in many contexts, but the underlying connections are not completely understood. To address this, we use paired genetic and computational screens in Drosophila to identify transcriptional regulators of immunity and pathology and their associated target genes and physiologies. We...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2013.09.007
更新日期:2013-10-10 00:00:00
abstract::Cardiomyocytes, the cells of the heart muscle, lose nearly all of their proliferative capacity after birth, limiting the heart's ability to regenerate. Naqvi et al. now identify a transient burst of cardiomyocyte proliferation during preadolescence, driven by a thyroid hormone surge, with therapeutic implications for ...
journal_title:Cell
pub_type: 评论,杂志文章
doi:10.1016/j.cell.2014.04.025
更新日期:2014-05-08 00:00:00
abstract::Immune responses in diverse tissue sites are critical for protective immunity and homeostasis. Here, we investigate how tissue localization regulates the development and function of human natural killer (NK) cells, innate lymphocytes important for anti-viral and tumor immunity. Integrating high-dimensional analysis of...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2020.01.022
更新日期:2020-02-20 00:00:00
abstract::Inducer T-cell clones reactive to the p-azobenzenearsonate (arsonate) hapten possess binding sites for radioactive arsanylated proteins, which are not present on clones with other antigen specificities. Binding occurred in the absence of histocompatibility proteins. Binding was specific for the p-azobenzenearsonate ha...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(84)90037-0
更新日期:1984-04-01 00:00:00
abstract::The V(D)J recombinase subunits Rag-1 and Rag-2 mediate assembly of antigen receptor gene segments. We studied the mechanisms of DNA recognition by Rag-1/Rag-2 using surface plasmon resonance. The critical step for signal recognition is binding of Rag-1 to the nonamer. This is achieved by a region of Rag-1 homologous t...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(00)81344-6
更新日期:1996-10-18 00:00:00
abstract::In familial searching in forensic genetics, a query DNA profile is tested against a database to determine whether it represents a relative of a database entrant. We examine the potential for using linkage disequilibrium to identify pairs of profiles as belonging to relatives when the query and database rely on nonover...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2018.09.008
更新日期:2018-10-18 00:00:00
abstract::Morphogen gradients are typically analyzed from static images of fixed embryonic tissues. Two papers in this issue of Cell now report live imaging of the Bicoid gradient in developing fruit fly embryos (Gregor et al., 2007a, 2007b). Their findings indicate that the gradient is highly reproducible from embryo to embryo...
journal_title:Cell
pub_type: 评论,杂志文章
doi:10.1016/j.cell.2007.06.036
更新日期:2007-07-13 00:00:00
abstract::Living organisms sense their physical environment through cellular mechanotransduction, which converts mechanical forces into electrical and biochemical signals. In turn, signal transduction serves a wide variety of functions, from basic cellular processes as diverse as proliferation, differentiation, migration, and a...
journal_title:Cell
pub_type: 杂志文章,评审
doi:10.1016/j.cell.2013.09.026
更新日期:2013-10-10 00:00:00
abstract::The human invariant chain (I gamma) of class II histocompatibility antigens spans the membrane of the endoplasmic reticulum once. It exposes a small amino-terminal domain on the cytoplasmic side and a carboxy-terminal, glycosylated domain on the exoplasmic side of the membrane. When the exoplasmic domain of I gamma is...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(86)90710-5
更新日期:1986-09-26 00:00:00
abstract::Nuclear and cytoplasmic poly(A)-binding proteins have been purified from Saccharomyces cerevisiae, and antisera have been used to isolate a gene that encodes them. The gene occurs in a single copy on chromosome 5 and gives rise to a unique, unspliced 2.1 kb transcript. The nuclear protein appears to be derived from th...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(86)90557-x
更新日期:1986-06-20 00:00:00
abstract::The Mre11-Rad50-Nbs1 (MRN) complex senses DNA double-strand breaks and recruits different repair pathway and checkpoint proteins to break foci. Two new studies (Williams et al., 2009; Lloyd et al., 2009) identify Nbs1 as a key factor in this process and reveal how an N-terminal protein recruitment module in Nbs1 binds...
journal_title:Cell
pub_type: 评论,杂志文章
doi:10.1016/j.cell.2009.09.017
更新日期:2009-10-02 00:00:00
abstract::Several recent papers show that differences in histone modification and the use of histone variants at the 5' and 3' ends of genes influence the location and kinetics of transcriptional initiation. The ultimate target of most epigenetic mechanisms may be the regulation of nucleosome occupancy, which in turn controls a...
journal_title:Cell
pub_type: 杂志文章,评审
doi:10.1016/j.cell.2005.12.010
更新日期:2005-12-29 00:00:00
abstract::To investigate the function of SWI/SNF in site-specific chromatin remodeling at promoters, we have used a purified system to analyze its distribution, function, and retention following recruitment by a sequence-specific transcription activator. Activator recruitment of SWI/SNF bound the complex to promoter proximal nu...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(01)00279-3
更新日期:2001-03-23 00:00:00
abstract::The pluripotent state of embryonic stem cells (ESCs) provides a unique perspective on regulatory programs that govern self-renewal and differentiation and somatic cell reprogramming. Here, we review the highly connected protein and transcriptional networks that maintain pluripotency and how they are intertwined with f...
journal_title:Cell
pub_type: 杂志文章,评审
doi:10.1016/j.cell.2011.05.019
更新日期:2011-06-10 00:00:00
abstract::Sleep is characterized by behavioral quiescence, homeostasis, increased arousal threshold, and rapid reversibility. Understanding how these properties are encoded by a neuronal circuit has been difficult, and no single molecular or neuronal pathway has been shown to be responsible for the regulation of sleep. Taking a...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2013.11.036
更新日期:2014-01-16 00:00:00
abstract::Tyrosine phosphorylation controls many cellular functions. Yet the three-part toolkit that regulates phosphotyrosine signaling-tyrosine kinases, phosphotyrosine phosphatases, and Src Homology 2 (SH2) domains-is a relatively new innovation. Genomic analyses reveal how this revolutionary signaling system may have origin...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2010.08.023
更新日期:2010-09-03 00:00:00
abstract::Lipid composition affects the biophysical properties of membranes that provide a platform for receptor-mediated cellular signaling. To study the regulatory role of membrane lipid composition, we combined genetic perturbations of sphingolipid metabolism with the quantification of diverse steps in Toll-like receptor (TL...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2015.05.051
更新日期:2015-07-02 00:00:00
abstract::The CD4 lymphocyte surface glycoprotein and the lck tyrosine protein kinase p56lck are found as a complex in T lymphocytes. We have defined the domains in both proteins that are responsible for this interaction by coexpressing hybrid and deleted forms of the two proteins in HeLa cells. We have found that the unique 32...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(89)90008-1
更新日期:1989-11-17 00:00:00
abstract::The cis-trans isomerization of proline serves as a regulatory switch in signaling pathways. We identify the proline isomerase Fpr4, a member of the FK506 binding protein family in Saccharomyces cerevisiae, as an enzyme which binds the amino-terminal tail of histones H3 and H4 and catalyses the isomerization of H3 prol...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2006.07.026
更新日期:2006-09-08 00:00:00
abstract::p25rum1 from the fission yeast S. pombe is shown to act as a specific in vitro inhibitor of the p34cdc2/p56cdc13 mitotic kinase. It is also shown that early G1 cells contain p25rum1, which associates with and inhibits the mitotic kinase, and maintains p56cdc13 mitotic B cyclin at a low level, ensuring that these cells...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(95)90215-5
更新日期:1995-12-15 00:00:00
abstract::The Wnt pathway controls cell fates, tissue homeostasis, and cancer. Its activation entails the association of beta-catenin with nuclear TCF/LEF proteins and results in transcriptional activation of target genes. The mechanism by which nuclear beta-catenin controls transcription is largely unknown. Here we genetically...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2006.01.053
更新日期:2006-04-21 00:00:00
abstract::The removal of apoptotic cells by phagocytic neighbors is essential for metazoan development but remains poorly characterized. Here we report the discovery of a Drosophila phagocytosis receptor, Six-microns-under (SIMU), which is expressed in highly phagocytic cell types during development and required for efficient a...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2008.02.052
更新日期:2008-05-02 00:00:00
abstract::Previous work on Drosophila santomea suggested that its absence of abdominal pigmentation, compared to the other darkly pigmented species, is based on mutations in the cis-regulatory region of tan, inactivating the expression of that gene in the abdomen of D. santomea males and females. Our discovery that D. santomea ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2009.10.033
更新日期:2009-12-11 00:00:00
abstract::Physiological concentrations of dihydrotestosterone (DHT) can specifically induce the release of fibrinolysin from androgen-dependent mammary carcinoma (Shionogi SC-115) cells in vitro. No fibrinolytic activity was observed in cells cultured either in the absence of DHT or presence of pharmacological concentrations of...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(76)90021-0
更新日期:1976-02-01 00:00:00
abstract::Senescence in Podospora anserina has long been shown to be under cytoplasmic control. Comparison of DNAs isolated from young and senescent cultures made it possible to detect the presence, in senescent cultures only, of a specific DNA (SEN-DNA). This DNA consists of repeated sequences arranged in a multimeric set of c...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(80)90126-9
更新日期:1980-08-01 00:00:00
abstract::Sexual reproduction is almost universal in eukaryotic life and involves the fusion of male and female haploid gametes into a diploid cell. The sperm-restricted single-pass transmembrane protein HAP2-GCS1 has been postulated to function in membrane merger. Its presence in the major eukaryotic taxa-animals, plants, and ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2017.01.024
更新日期:2017-02-23 00:00:00