Abstract:
:To investigate the role of the presumed DNA mismatch repair (MMR) gene Msh2 in genome stability and tumorigenesis, we have generated cells and mice that are deficient for the gene. Msh2-deficient cells have lost mismatch binding and have acquired microsatellite instability, a mutator phenotype, and tolerance to methylating agents. Moreover, in these cells, homologous recombination has lost dependence on complete identity between interacting DNA sequences, suggesting that Msh2 is involved in safeguarding the genome from promiscuous recombination. Msh2-deficient mice display no major abnormalities, but a significant fraction develops lymphomas at an early age. Thus, Msh2 is involved in MMR, controlling several aspects of genome stability; loss of MMR-controlled genome stability predisposes to cancer.
journal_name
Celljournal_title
Cellauthors
de Wind N,Dekker M,Berns A,Radman M,te Riele Hdoi
10.1016/0092-8674(95)90319-4subject
Has Abstractpub_date
1995-07-28 00:00:00pages
321-30issue
2eissn
0092-8674issn
1097-4172pii
0092-8674(95)90319-4journal_volume
82pub_type
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