Abstract：
:The Gin DNA invertase of bacteriophage Mu carries out processive recombination in which multiple rounds of exchange follow synaptic complex formation. The stereostructure of the knotted products determined by electron microscopy establishes critical features of site synapsis and DNA exchange. Surprisingly, the invertase knots substrates with directly repeated sites as well as those with inverted sites. The results suggest that the Gin synaptic complex contains three mutually perpendicular dyads; one is the axis of site rotation during exchange, and they cause inverted and direct site substrates to form a similar synaptic complex. The extensive knotting by Gin has implications for the energetics of recombination and shows that the enhancer for recombination is required only at an early stage, and thus may normally operate in a hit-and-run fashion.
journal_name
Celljournal_title
Cellauthors
Kanaar R,Klippel A,Shekhtman E,Dungan JM,Kahmann R,Cozzarelli NRdoi
10.1016/0092-8674(90)90372-lsubject
Has Abstractpub_date
1990-07-27 00:00:00pages
353-66issue
2eissn
0092-8674issn
1097-4172pii
0092-8674(90)90372-Ljournal_volume
62pub_type
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