Abstract：
:The triskelion shape of the clathrin molecule enables it to form the polyhedral protein network that covers clathrin-coated pits and vesicles. Domains within the clathrin heavy chain that are responsible for maintaining triskelion shape and function were identified and localized. Sequences that mediate trimerization are distal to the carboxyl terminus and are adjacent to a domain that mediates both light chain binding and clathrin assembly. Structural modeling predicts that within this domain, the region of heavy chain-light chain interaction is a bundle of three or four alpha helices. These studies establish a low resolution model of clathrin subunit folding in the central portion (hub) of the triskelion, thus providing a basis for future mutagenesis experiments.
journal_name
Celljournal_title
Cellauthors
Näthke IS,Heuser J,Lupas A,Stock J,Turck CW,Brodsky FMdoi
10.1016/0092-8674(92)90033-9subject
Has Abstractpub_date
1992-03-06 00:00:00pages
899-910issue
5eissn
0092-8674issn
1097-4172pii
0092-8674(92)90033-9journal_volume
68pub_type
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