Single-Molecule Visualization of MCM2-7 DNA Loading: Seeing Is Believing.

abstract：:While the nuclear lamin proteins (A, B, and C) assemble specifically at the surface of the nuclear membrane, their sequences do not reveal stretches of hydrophobic amino acids that might explain their association with the nuclear membranes. However, the A and B lamin proteins possess Ras-like C-terminal CaaX sequence ...

journal_title：Cell

authors： Holtz D,Tanaka RA,Hartwig J,McKeon F

更新日期：1989-12-22 00:00:00

abstract：:Primary cilium dysfunction underlies the pathogenesis of Bardet-Biedl syndrome (BBS), a genetic disorder whose symptoms include obesity, retinal degeneration, and nephropathy. However, despite the identification of 12 BBS genes, the molecular basis of BBS remains elusive. Here we identify a complex composed of seven h...

journal_title：Cell

authors： Nachury MV,Loktev AV,Zhang Q,Westlake CJ,Peränen J,Merdes A,Slusarski DC,Scheller RH,Bazan JF,Sheffield VC,Jackson PK

更新日期：2007-06-15 00:00:00

abstract：:Plasmids containing the origin region of human mtDNA are specifically transcribed by the partially purified homologous mtRNA polymerase in vitro. Transcription of both the light and heavy strands initiates at the same sites previously identified as in vivo transcription start sites. The sequences responsible for initi...

journal_title：Cell

authors： Bogenhagen DF,Applegate EF,Yoza BK

更新日期：1984-04-01 00:00:00

abstract：:We report here the identification of thyroid hormone response elements (TREs) that consist of a direct repeat, not a palindrome, of the half-sites. Unlike palindromic TREs, direct repeat TREs do not confer a retinoic acid response. The tandem TRE can be converted into a retinoic acid response element by increasing the...

journal_title：Cell

authors： Umesono K,Murakami KK,Thompson CC,Evans RM

更新日期：1991-06-28 00:00:00

abstract：:Cholera toxin is assembled from two subunits in the periplasm of Vibrio cholerae and disassembled in the analogous compartment of target cells, the lumen of the endoplasmic reticulum (ER), before a fragment of it, the A1 chain, is transported into the cytosol. We show that protein disulfide isomerase (PDI) in the ER l...

journal_title：Cell

authors： Tsai B,Rodighiero C,Lencer WI,Rapoport TA

更新日期：2001-03-23 00:00:00

abstract：:Motor neurons stimulate their postsynaptic muscle targets to synthesize neurotransmitter receptors. Polypeptide signaling molecules may mediate this inductive interaction. Here we report the purification of ARIA, a protein that stimulates the synthesis of muscle acetylcholine receptors, and the isolation of ARIA cDNA....

journal_title：Cell

authors： Falls DL,Rosen KM,Corfas G,Lane WS,Fischbach GD

更新日期：1993-03-12 00:00:00

abstract：:During eukaryotic translation initiation, initiator tRNA does not insert fully into the P decoding site on the 40S ribosomal subunit. This conformation (POUT) is compatible with scanning mRNA for the AUG start codon. Base pairing with AUG is thought to promote isomerization to a more stable conformation (PIN) that arr...

journal_title：Cell

authors： Hussain T,Llácer JL,Fernández IS,Munoz A,Martin-Marcos P,Savva CG,Lorsch JR,Hinnebusch AG,Ramakrishnan V

更新日期：2014-10-23 00:00:00

abstract：:The mechanism by which the membrane proteins of Sindbis virus are initially glycosylated during growth of the virus in chick cells was studied. The experiments suggest strongly that the two viral glycoproteins are glycosylated before release from the polysome, and that this glycosylation involves transfer of a large 1...

journal_title：Cell

authors： Sefton BM

更新日期：1977-04-01 00:00:00

abstract：:The tumor microenvironment is known to play an important role in tumorigenesis. In this issue, Yang et al. (2008) demonstrate that mast cells heterozygous for the Nf1 gene promote the growth of neurofibromas in a mouse model of neurofibromatosis and that genetic and pharmacological inhibition of these cells is suffici...

journal_title：Cell

authors： Reilly KM,Van Dyke T

更新日期：2008-10-31 00:00:00

abstract：:Serum response factor (SRF) forms a ternary complex at the c-fos serum response element (SRE) with an accessory factor, Elk-1. We constructed altered-binding specificity derivatives of SRF and Elk-1 that form a ternary complex at a mutated, inactive SRE; like Elk-1, the Elk-1 variant only binds its target as part of a...

journal_title：Cell

authors： Hill CS,Marais R,John S,Wynne J,Dalton S,Treisman R

更新日期：1993-04-23 00:00:00

abstract：:Replicative intermediates of SV40 were isolated, digested with the restriction endonuclease Bgl I and examined by electron microscopy. Over 98% of the replicative intermediates isolated following infection with wild-type virions at 33 degrees, 37 degrees or 40 degrees C or with tsA209 at 33 degrees C had initiated rep...

journal_title：Cell

authors： Martin RG,Setlow VP

更新日期：1980-06-01 00:00:00

abstract：:Brassinosteroids (BRs) signal through a plasma membrane-localized receptor kinase to regulate plant growth and development. We showed previously that a novel protein, BES1, accumulates in the nucleus in response to BRs, where it plays a role in BR-regulated gene expression; however, the mechanism by which BES1 regulat...

journal_title：Cell

authors： Yin Y,Vafeados D,Tao Y,Yoshida S,Asami T,Chory J

更新日期：2005-01-28 00:00:00

abstract：:Muscle atrophy (cachexia) in cancer patients is a life-threatening condition for which therapeutic options are limited. Zhou et al. (2010) now identify a new target for treating cachexia, the activin type-2 receptor (ActRIIB). In several mouse models of cachexia, the authors reversed wasting of skeletal and cardiac mu...

journal_title：Cell

authors： Tisdale MJ

更新日期：2010-08-20 00:00:00

abstract：:Developmental analysis of a newly isolated maternal effect grandchildless mutant, tudor (tud), in Drosophila melanogaster indicates that tud+ activity is required during oogenesis for the determination and/or formation of primordial germ cells (pole cells) and for normal embryonic abdominal segmentation. Regardless of...

journal_title：Cell

authors： Boswell RE,Mahowald AP

更新日期：1985-11-01 00:00:00

abstract：:Brefeldin A (BFA) is a fungal metabolite that causes a redistribution of the stacked cisternae of the Golgi complex into the endoplasmic reticulum by inhibiting anterograde transport. We report that BFA also causes membrane tubules derived from the trans-Golgi network (TGN) to fuse with early endosomes. In the presenc...

journal_title：Cell

authors： Wood SA,Park JE,Brown WJ

更新日期：1991-11-01 00:00:00

abstract：:Expression of pipe in the somatic tissue of the Drosophila ovary is required for the formation of embryonic dorsal-ventral polarity. pipe, which encodes an enzyme similar to the glycosaminoglycan-modifying enzyme heparan sulfate 2-O-sulfotransferase, is expressed in a spatially restricted domain of follicle cells on t...

journal_title：Cell

authors： Sen J,Goltz JS,Stevens L,Stein D

更新日期：1998-11-13 00:00:00

abstract：:Dietary soluble fibers are fermented by gut bacteria into short-chain fatty acids (SCFA), which are considered broadly health-promoting. Accordingly, consumption of such fibers ameliorates metabolic syndrome. However, incorporating soluble fiber inulin, but not insoluble fiber, into a compositionally defined diet, ind...

journal_title：Cell

authors： Singh V,Yeoh BS,Chassaing B,Xiao X,Saha P,Aguilera Olvera R,Lapek JD Jr,Zhang L,Wang WB,Hao S,Flythe MD,Gonzalez DJ,Cani PD,Conejo-Garcia JR,Xiong N,Kennett MJ,Joe B,Patterson AD,Gewirtz AT,Vijay-Kumar M

更新日期：2018-10-18 00:00:00

abstract：:Apoptosis induced by TNF-receptor I (TNFR1) is thought to proceed via recruitment of the adaptor FADD and caspase-8 to the receptor complex. TNFR1 signaling is also known to activate the transcription factor NF-kappa B and promote survival. The mechanism by which this decision between cell death and survival is arbitr...

journal_title：Cell

authors： Micheau O,Tschopp J

更新日期：2003-07-25 00:00:00

abstract：:Determining protein levels in each tissue and how they compare with RNA levels is important for understanding human biology and disease as well as regulatory processes that control protein levels. We quantified the relative protein levels from over 12,000 genes across 32 normal human tissues. Tissue-specific or tissue...

journal_title：Cell

authors： Jiang L,Wang M,Lin S,Jian R,Li X,Chan J,Dong G,Fang H,Robinson AE,GTEx Consortium.,Snyder MP

更新日期：2020-10-01 00:00:00

abstract：:A growing body of evidence indicates that plant transcription factors move between cells. A recent paper by Nakajima et al. (2001) shows that movement of the SHORTROOT protein provides a mechanism for signaling positional information between cell layers of the root. ...

journal_title：Cell

authors： Barton MK

更新日期：2001-10-19 00:00:00

abstract：:Lineage tracing is the identification of all progeny of a single cell. Although its origins date back to developmental biology of invertebrates in the 19(th) century, lineage tracing is now an essential tool for studying stem cell properties in adult mammalian tissues. Lineage tracing provides a powerful means of unde...

journal_title：Cell

authors： Kretzschmar K,Watt FM

更新日期：2012-01-20 00:00:00

abstract：:The switch from short-term to long-term facilitation of the synapses between sensory and motor neurons mediating gill and tail withdrawal reflexes in Aplysia requires CREB-mediated transcription and new protein synthesis. We isolated several downstream genes, one of which encodes a neuron-specific ubiquitin C-terminal...

journal_title：Cell

authors： Hegde AN,Inokuchi K,Pei W,Casadio A,Ghirardi M,Chain DG,Martin KC,Kandel ER,Schwartz JH

更新日期：1997-04-04 00:00:00

abstract：:The actin-filled stereocilia of cochlear hair cells deflect in response to sound by pivoting around rootlets at their insertion points at the sensory cell surface. Kitajiri et al. (2010) now show that the actin-binding protein TRIOBP tightly bundles the actin filaments into rootlets, endowing these stereocilia "pivots...

journal_title：Cell

authors： Boutet de Monvel J,Petit C

更新日期：2010-05-28 00:00:00

abstract：:When treated with the synthetic glucocorticoid dexamethasone, HT1080 human fibrosarcoma cells show changes in morphology, adhesion, and the extracellular matrix. Dexamethasone treatment results in a tenfold increase in the rate of fibronectin biosynthesis in HT1080 cells and a twofold increase in untransformed, normal...

journal_title：Cell

authors： Oliver N,Newby RF,Furcht LT,Bourgeois S

更新日期：1983-05-01 00:00:00

abstract：:We studied H1 gene expression in hybrids of Xenopus laevis (female) x Xenopus borealis (male) and saw paternal H1 synthesis in mid-blastulae, which indicates that the H1 genes are active by this stage. The behavior of the maternal store of H1 histone mRNA was studied in androgenetic haploids. In which all stored mRNA ...

journal_title：Cell

authors： Woodland HR,Flynn JM,Wyllie AJ

更新日期：1979-09-01 00:00:00

abstract：:Trigger factor (TF) is a molecular chaperone that binds to bacterial ribosomes where it contacts emerging nascent chains, but TF is also abundant free in the cytosol where its activity is less well characterized. In vitro studies show that TF promotes protein refolding. We find here that ribosome-free TF stably associ...

journal_title：Cell

authors： Martinez-Hackert E,Hendrickson WA

更新日期：2009-09-04 00:00:00

abstract：:The yeast Sir2/3/4p complex is found in abundance at telomeres, where it participates in the formation of silent heterochromatin and telomere maintenance. Here, we show that Sir3p is released from telomeres in response to DNA double-strand breaks (DSBs), binds to DSBs, and mediates their repair, independent of cell ma...

journal_title：Cell

authors： Mills KD,Sinclair DA,Guarente L

更新日期：1999-05-28 00:00:00

abstract：:E. coli RecA protein promotes homologous pairing and reciprocal strand exchange reactions between duplex DNA molecules in vitro. Reaction intermediates contain Holliday junctions that are driven along the DNA at a maximal rate approaching 1000 bases per minute. T4 endonuclease VII cleaves Holliday junctions in vitro, ...

journal_title：Cell

authors： Müller B,Jones C,Kemper B,West SC

更新日期：1990-01-26 00:00:00

abstract：:The alpha-amylase mRNAs which accumulate in two different tissues of the mouse, the salivary gland and the liver, are identical except for their 5' non-translated sequences: the 5' terminal 158 nucleotides of the major liver alpha-amylase mRNA are unrelated to the 5' terminal 47 nucleotides found in its salivary gland...

journal_title：Cell

authors： Young RA,Hagenbüchle O,Schibler U

更新日期：1981-02-01 00:00:00

abstract：:An acute but transient response to insulin is essential for glucose homeostasis in mammals. Chakraborty et al. (2010) uncover a new feedback mechanism regulating insulin signaling. They show that the inositol pyrophosphate IP7, which is produced in response to insulin, inhibits the Akt kinase, a primary effector of in...

journal_title：Cell

authors： Manning BD

更新日期：2010-12-10 00:00:00