Abstract：
:Primary cilium dysfunction underlies the pathogenesis of Bardet-Biedl syndrome (BBS), a genetic disorder whose symptoms include obesity, retinal degeneration, and nephropathy. However, despite the identification of 12 BBS genes, the molecular basis of BBS remains elusive. Here we identify a complex composed of seven highly conserved BBS proteins. This complex, the BBSome, localizes to nonmembranous centriolar satellites in the cytoplasm but also to the membrane of the cilium. Interestingly, the BBSome is required for ciliogenesis but is dispensable for centriolar satellite function. This ciliogenic function is mediated in part by the Rab8 GDP/GTP exchange factor, which localizes to the basal body and contacts the BBSome. Strikingly, Rab8(GTP) enters the primary cilium and promotes extension of the ciliary membrane. Conversely, preventing Rab8(GTP) production blocks ciliation in cells and yields characteristic BBS phenotypes in zebrafish. Our data reveal that BBS may be caused by defects in vesicular transport to the cilium.
journal_name
Celljournal_title
Cellauthors
Nachury MV,Loktev AV,Zhang Q,Westlake CJ,Peränen J,Merdes A,Slusarski DC,Scheller RH,Bazan JF,Sheffield VC,Jackson PKdoi
10.1016/j.cell.2007.03.053subject
Has Abstractpub_date
2007-06-15 00:00:00pages
1201-13issue
6eissn
0092-8674issn
1097-4172pii
S0092-8674(07)00534-Xjournal_volume
129pub_type
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