Abstract：
:Mammals can taste a wide repertoire of chemosensory stimuli. Two unrelated families of receptors (T1Rs and T2Rs) mediate responses to sweet, amino acids, and bitter compounds. Here, we demonstrate that knockouts of TRPM5, a taste TRP ion channel, or PLCbeta2, a phospholipase C selectively expressed in taste tissue, abolish sweet, amino acid, and bitter taste reception, but do not impact sour or salty tastes. Therefore, despite relying on different receptors, sweet, amino acid, and bitter transduction converge on common signaling molecules. Using PLCbeta2 taste-blind animals, we then examined a fundamental question in taste perception: how taste modalities are encoded at the cellular level. Mice engineered to rescue PLCbeta2 function exclusively in bitter-receptor expressing cells respond normally to bitter tastants but do not taste sweet or amino acid stimuli. Thus, bitter is encoded independently of sweet and amino acids, and taste receptor cells are not broadly tuned across these modalities.
journal_name
Celljournal_title
Cellauthors
Zhang Y,Hoon MA,Chandrashekar J,Mueller KL,Cook B,Wu D,Zuker CS,Ryba NJdoi
10.1016/s0092-8674(03)00071-0subject
Has Abstractpub_date
2003-02-07 00:00:00pages
293-301issue
3eissn
0092-8674issn
1097-4172pii
S0092867403000710journal_volume
112pub_type
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