Abstract：
:We have isolated spontaneous temperature-resistant revertants of a temperature-sensitive mutation (rpoD800) in the sigma subunit of E. coli K12 RNA polymerase. These revertants still contained the rpoD800 allele. They were mucoid, and sensitive to ultraviolet light and the radiomimetic agent nitrofurantoin, which are characteristics of lon mutants. One revertant, Tr29, was mapped to the lon region of the chromosome. Lon- rpoD800 double mutants were constructed, and were phenotypically indistinguishable from the spontaneous temperature-resistant revertant. It is the degradation-deficient property of lon mutants that is responsible for the suppression of the temperature-sensitive phenotype. We show that the rpoD800 sigma polypeptide is a substrate for the ion proteolytic system, and that mutations in lon decrease the rate of mutant sigma degradation. The rate of synthesis of mutant sigma was also affected in lon- strains. The net effect of lon-mutations was to increase the concentration of mutant sigma. We conclude that the temperature-sensitive phenotype results from insufficient concentration, rather than altered function, of the mutant protein.
journal_name
Celljournal_title
Cellauthors
Grossman AD,Burgess RR,Walter W,Gross CAdoi
10.1016/0092-8674(83)90505-6subject
Has Abstractpub_date
1983-01-01 00:00:00pages
151-9issue
1eissn
0092-8674issn
1097-4172pii
0092-8674(83)90505-6journal_volume
32pub_type
杂志文章相关文献
CELL文献大全abstract：:Autophagy has been associated with both cell survival and cell death, but the role of autophagy in cell death has been controversial. In this issue, Berry and Baehrecke (2007) report that autophagy is involved in physiological cell death during Drosophila development and is controlled by similar mechanisms as those th...
journal_title：Cell
pub_type： 评论,杂志文章
doi：10.1016/j.cell.2007.11.027
更新日期：2007-12-14 00:00:00
abstract：:The association of histone modification changes with autism spectrum disorder (ASD) has not been systematically examined. We conducted a histone acetylome-wide association study (HAWAS) by performing H3K27ac chromatin immunoprecipitation sequencing (ChIP-seq) on 257 postmortem samples from ASD and matched control brai...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2016.10.031
更新日期：2016-11-17 00:00:00
abstract：:Plasma membrane vesicles and protoplasts of Escherichia coli were fused to planar lipid bilayers and studied with electrophysiological techniques. Large transmembrane aqueous channels were opened when 0.2 nM LamB signal peptide was added to the cytoplasmic side of the membrane. These aqueous pores are similar in condu...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(92)90231-z
更新日期：1992-05-15 00:00:00
abstract：:Polyadenylation of mRNA precursors by poly(A) polymerase depends on a specificity factor, CPF, recognizing the polyadenylation signal AAUAAA. This paper describes an apparently novel poly(A)-binding protein that acts as a second specificity factor, mediating the recognition of the growing poly(A) tail. A transition fr...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(91)90119-j
更新日期：1991-08-23 00:00:00
abstract：:In this report, we describe the isolation, sequence, and initial characterization of the cDNA for the muscle-specific regulatory factor skeletal myogenin. Transfection of myogenin into the mesenchymal cell line C3H10T1/2 produces cells expressing muscle-specific markers. Myogenin is absent in undifferentiated cells, p...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(89)90583-7
更新日期：1989-02-24 00:00:00
abstract：:Recent evidence suggests that a subset of cancer cells within some tumors, the so-called cancer stem cells, may drive the growth and metastasis of these tumors. Understanding the pathways that regulate proliferation, self-renewal, survival, and differentiation of malignant and normal stem cells may shed light on mecha...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2006.03.011
更新日期：2006-03-24 00:00:00
abstract：:Regulated transcription of the prospero gene in the Drosophila eye provides a model for how gene expression is specifically controlled by signals from receptor tyrosine kinases. We show that prospero is controlled by signals from the EGF receptor DER and the Sevenless receptor. A direct link is established between DER...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(00)00107-0
更新日期：2000-09-29 00:00:00
abstract：:The triskelion shape of the clathrin molecule enables it to form the polyhedral protein network that covers clathrin-coated pits and vesicles. Domains within the clathrin heavy chain that are responsible for maintaining triskelion shape and function were identified and localized. Sequences that mediate trimerization a...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(92)90033-9
更新日期：1992-03-06 00:00:00
abstract：:Individual olfactory sensory neurons express only a single odorant receptor from a large family of genes, and this singularity is an essential feature in models of olfactory perception. We have devised a genetic strategy to examine the stability of receptor choice. We observe that immature olfactory sensory neurons th...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2004.05.015
更新日期：2004-06-11 00:00:00
abstract：:A recombinant peptide encoding the CD11b A domain bound 54Mn2+ with a high affinity. Other divalent cations, including Mg2+, Zn2+, Ni2+, Co2+, and Cd2+, but not Ca2+ or Ba2+, competed effectively for Mn2+ binding. Amino acid substitutions within two conserved and noncontiguous regions in the recombinant peptide abolis...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(93)90575-b
更新日期：1993-03-26 00:00:00
abstract：:Most bacteria that colonize eukaryotes must bind directly to host cells to establish a replicative niche. In enteric bacteria, adhesion to host cells is often promoted by a lectin found on surface-localized pili. Some pili promote efficient adhesion only when they are subjected to shear stress, as found during the flo...
journal_title：Cell
pub_type： 杂志文章,评审
doi：10.1016/s0092-8674(02)00821-8
更新日期：2002-07-12 00:00:00
abstract：:As more plant genome sequences become available, researchers are increasingly using comparative genomics to address some of the major questions in plant biology. Such questions include the evolution of photosynthesis and multicellularity, the developmental genetic changes responsible for alterations in body plan, and ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2007.04.004
更新日期：2007-04-20 00:00:00
abstract：:The synthesis and secretion of plasminogen activator by cultured macrophages can be induced and stimulated by concanavalin A and by phorbol myristate acetate, and inhibited by such agents as glucocorticoids, mitotic inhibitors and compounds affecting cAMP metabolism. By the manipulation of stimulatory and inhibitory i...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(77)90086-1
更新日期：1977-07-01 00:00:00
abstract：:ARF GTPases act at multiple steps of the secretory and vacuolar/lysosomal trafficking pathways, but little is known about the spatial regulation of ARF activation. In this issue of Cell, Geldner et al. demonstrate that the Arabidopsis ARF activator GNOM localizes to endosomes where it controls the polarized traffickin...
journal_title：Cell
pub_type： 评论,杂志文章
doi：10.1016/s0092-8674(03)00038-2
更新日期：2003-01-24 00:00:00
abstract：:AMPA receptors mediate fast excitatory neurotransmission in the mammalian brain and transduce the binding of presynaptically released glutamate to the opening of a transmembrane cation channel. Within the postsynaptic density, however, AMPA receptors coassemble with transmembrane AMPA receptor regulatory proteins (TAR...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2017.07.045
更新日期：2017-09-07 00:00:00
abstract：:S. cerevisiae inherits its vacuole by projecting vacuole-derived membrane vesicles and tubules into the bud, where they fuse to establish the daughter vacuole. This homotypic fusion event can be assayed in vitro. It requires Sec17p and Sec18p, the homologs of the mammalian alpha-SNAP and NSF, which cooperate in multip...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(00)81084-3
更新日期：1996-04-05 00:00:00
abstract：:Cell-cell junctions respond to mechanical forces by changing their organization and function. To gain insight into the mechanochemical basis underlying junction mechanosensitivity, we analyzed tight junction (TJ) formation between the enveloping cell layer (EVL) and the yolk syncytial layer (YSL) in the gastrulating z...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2019.10.006
更新日期：2019-10-31 00:00:00
abstract：:The precursor of the 55K adenovirus terminal protein is an 87K protein that is covalently linked to viral DNA. This protein is likely to be identical to the 80,000 dalton protein described by Challberg et al. (1980). The mRNA for the 87K terminal protein precursor, like that for the E2-72K DNA binding protein, is dete...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(81)90145-8
更新日期：1981-02-01 00:00:00
abstract：:Recent studies suggest that local translation of mRNA within axons plays a key role in two aspects of growth cone navigation. One line of evidence implies a local synthesis of proteins that mediate growth cone collapse and turning in response to guidance cues; another indicates that growth cones possess the machinery ...
journal_title：Cell
pub_type： 杂志文章,评审
doi：10.1016/s0092-8674(02)00934-0
更新日期：2002-09-06 00:00:00
abstract：:While hematopoietic stem cell (HSC) self-renewal is well studied, it remains unknown whether distinct control mechanisms enable HSC divisions that generate progeny cells with specific lineage bias. Here, we report that the monocytic transcription factor MafB specifically restricts the ability of M-CSF to instruct myel...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2009.04.057
更新日期：2009-07-23 00:00:00
abstract：:Release of CHO-K1 cells from plateau or stationary phase and reentry into the cell cycle is specifically and reversibly blocked at two distinct sites by the anticalmodulin drug W13. The first block occurs early during release while the cells are still at G0/G1, whereas the second occurs later in reentry during early S...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(84)90075-8
更新日期：1984-01-01 00:00:00
abstract：:The phage Mu transpososome is assembled by interactions of transposase subunits with the left (L) and right (R) ends of Mu and an enhancer (E) located in between. A metastable three-site complex LER progresses into a more stable type 0 complex in which a tetrameric transposase is poised for DNA cleavage. "Difference t...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(02)00728-6
更新日期：2002-05-17 00:00:00
abstract：:Homology is an important feature of recombination. We have used the rll cistrons of bacteriophage T4 to determine the extent of homology required for recombination. We varied the amount of homologous DNA available for recombination in both marker rescue experiments and deletion-by-deletion crosses. Our results suggest...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(82)90401-9
更新日期：1982-11-01 00:00:00
abstract：:Newly synthesized transcripts are usually spliced during transcription or immediately thereafter. So pre-mRNA splicing has been presumed to occur exclusively in the cell nucleus. In this issue of Cell, Denis et al. (2005) now report the presence of functional spliceosomes and signal-dependent pre-mRNA splicing in the ...
journal_title：Cell
pub_type： 评论,杂志文章,评审
doi：10.1016/j.cell.2005.07.016
更新日期：2005-08-12 00:00:00
abstract：:The location of cis-acting regulatory sequences within the long terminal repeat (LTR) of the human T cell lymphotropic virus type III (HTLV-III/LAV) was determined. An enhancer element capable of increasing the rate of transcription from a heterologous promoter, irrespective of distance and orientation, is located bet...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(85)80062-3
更新日期：1985-07-01 00:00:00
abstract：:The adenovirus E1a region encodes two closely related gene products: 243 and 289 amino acid phosphoproteins. These proteins differ in their primary sequence only by 46 amino acids unique to the 289 amino acid protein. By constructing single-base substitution mutants we localized two functional regions of these E1a pro...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(86)90704-x
更新日期：1986-09-26 00:00:00
abstract：:The short chain aliphatic acid salts, butyrate and propionate, are effective inhibitors of histone deacetylation in chick oviduct at 2--5 mM; they also prevent the hormonal induction of the ovalbumin and transferrin genes. The less potent deacetylase inhibitor isobutyrate is correspondingly less effective in blocking ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(80)90357-8
更新日期：1980-11-01 00:00:00
abstract：:Of the several families of adhesion receptors involved in leukocyte-endothelial cell interactions, only the selectins have been shown to initiate leukocyte interaction under physiologic shear; indeed, beta 2 (CD18) intergrins responsible for neutrophil arrest are unable to engage without prior selectin-mediated rollin...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(95)90491-3
更新日期：1995-02-10 00:00:00
abstract：:The discovery that somatic cells can be reprogrammed into induced pluripotent stem cells (iPSCs) raised the exciting possibility of modeling diseases with patient-specific cells. Marchetto et al. (2010) now use iPSC technology to generate, characterize, and treat an in vitro model for the autism spectrum disorder Rett...
journal_title：Cell
pub_type： 评论,杂志文章
doi：10.1016/j.cell.2010.10.037
更新日期：2010-11-12 00:00:00
abstract：:Polycomb group (PcG) and trithorax group (trxG) proteins are critical regulators of numerous developmental genes. To silence or activate gene expression, respectively, PcG and trxG proteins bind to specific regions of DNA and direct the posttranslational modification of histones. Recent work suggests that PcG proteins...
journal_title：Cell
pub_type： 杂志文章,评审
doi：10.1016/j.cell.2007.02.009
更新日期：2007-02-23 00:00:00
