Abstract:
:A general strategy is described for the isolation of suppressors of essential genes whose functions are unknown. This strategy was used to analyze the role of 4.5S RNA, an essential RNA of E. coli. In this strategy, the structural gene for 4.5S RNA is fused to the Ptac promoter in such a way that the strain becomes dependent upon inducers of lac for growth. Mutants mapping to fus, the structural gene for protein synthesis elongation factor G, appear as spontaneous, inducer-independent revertants. These mutants alter the intracellular distribution of 4.5S RNA such that it sediments at 70S or greater. Furthermore, the increased sedimentation velocity is sensitive to the antibiotic puromycin. These results show that 4.5S RNA physically associates with the ribosome in performing its essential function, and that this association is mediated by elongation factor G.
journal_name
Celljournal_title
Cellauthors
Brown Sdoi
10.1016/0092-8674(87)90620-9subject
Has Abstractpub_date
1987-06-19 00:00:00pages
825-33issue
6eissn
0092-8674issn
1097-4172pii
0092-8674(87)90620-9journal_volume
49pub_type
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