Abstract:
:It was previously demonstrated that the 60,000 dalton (p60) precursor-like polyprotein containing murine p30 was a constituent of the feline leukemia virus pseudotype of Moloney sarcoma virus [m1MSV(FeLV)]. It is now shown that p60 is detected in cells of five mammalian species transformed by m1MSV, indicating that p60 is specified by this genome. Moreover, little or no murine p30 is detected in the m1MSV-transformed cells, suggesting that the murine group p30 antigenic reactivity of S + L- cells is ude to p60. Pulse-chase studies in cells producing m1MSV(FeLV) show that p60 is the largest polypeptide detectable during the pulse, and that intracellular p60 is not cleaved into smaller (for example, p30) polypeptides during chase periods of up to 10 hr. The lack of cleavage of p60 is in contrast to the properties of p30 precursors detected in cells containing replicating avian or mammalian RNA tumor viruses. The inefficient cleavage of intracellular p60 and the kinetics of appearance of murine p30 in extracellular m1MSV(FeLV) suggest that p60 cleavage to p30 occurs in cells shortly before virus release. While only p60 was detected in the m1MSV-transformed cells, p60 and p70 were detected in m3MSV-transformed cells, and no immunoprecipitable polypeptides were detected in HT-1 MSV-transformed cells. The observed differences in the intracellular polypeptide expression by each of the strains of MSV suggests differences in genetic content.
journal_name
Celljournal_title
Cellauthors
Robey WG,Oskarsson MK,Vande Woude GF,Naso RB,Arlinghaus RB,Haapala DK,Fischinger PJdoi
10.1016/0092-8674(77)90142-8subject
Has Abstractpub_date
1977-01-01 00:00:00pages
79-89issue
1eissn
0092-8674issn
1097-4172pii
0092-8674(77)90142-8journal_volume
10pub_type
杂志文章相关文献
CELL文献大全abstract::E. coli sigma 54 protein confers on promoters containing its recognition sequence the ability to be activated from distant DNA sites. Its functional domains include two leucine zipper motifs, an acidic region, and a glutamine-rich domain. Several domains were disrupted and the assembly of mutant transcription complexe...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(90)90269-k
更新日期:1990-09-07 00:00:00
abstract::Mutations at the mouse tottering (tg) locus cause a delayed-onset, recessive neurological disorder resulting in ataxia, motor seizures, and behavioral absence seizures resembling petit mal epilepsy in humans. A more severe allele, leaner (tg(la)), also shows a slow, selective degeneration of cerebellar neurons. By pos...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(00)81381-1
更新日期:1996-11-15 00:00:00
abstract::How and when memory T cells form during an immune response are long-standing questions. To better understand memory CD8 T cell development, a time course of gene expression and functional changes in antigen-specific T cells during viral infection was evaluated. The expression of many genes continued to change after vi...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(02)01139-x
更新日期:2002-12-13 00:00:00
abstract::Microbes that cause persistent infections (e.g., herpes viruses) do so by switching from fast-growing lytic states to slow-growing latent states. Waldman et al. have identified a single transcription factor that governs the switch between the lytic and latent forms of Toxoplasma gondii, a parasite that causes a persis...
journal_title:Cell
pub_type: 评论,杂志文章
doi:10.1016/j.cell.2019.12.038
更新日期:2020-01-23 00:00:00
abstract::The scrapie (PrPSc) and cellular (PrPC) prion proteins are encoded by the same gene, and their different properties are thought to arise from posttranslational modifications. We have found a phosphatidylinositol glycolipid on both PrPC and PrP 27-30 (derived from PrPSc by limited proteolysis at the amino terminus). Et...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(87)90150-4
更新日期:1987-10-23 00:00:00
abstract::DNA transcription, replication, and repair are regulated by histone acetylation, a process that requires the generation of acetyl-coenzyme A (CoA). Here, we show that all the subunits of the mitochondrial pyruvate dehydrogenase complex (PDC) are also present and functional in the nucleus of mammalian cells. We found t...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2014.04.046
更新日期:2014-07-03 00:00:00
abstract::Nijmegen breakage syndrome (NBS) is an autosomal recessive chromosomal instability syndrome characterized by microcephaly, growth retardation, immunodeficiency, and cancer predisposition. Cells from NBS patients are hypersensitive to ionizing radiation with cytogenetic features indistinguishable from ataxia telangiect...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(00)81174-5
更新日期:1998-05-01 00:00:00
abstract::A glycoprotein of molecular weight 130,000 (gP130) has been precipitated from the cytoplasm of GR-strain mouse mammary tumor (GR-MMT) cells by a rabbit antiserum (anti-MMTV) to GR-strain mouse mammary tumor virus (GR-MMTV). This protein was not precipitated by antisera specific for detergent-disrupted C3H-strain MMTV ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(80)90447-x
更新日期:1980-10-01 00:00:00
abstract::Large numbers of inbred laboratory rat strains have been developed for a range of complex disease phenotypes. To gain insights into the evolutionary pressures underlying selection for these phenotypes, we sequenced the genomes of 27 rat strains, including 11 models of hypertension, diabetes, and insulin resistance, al...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2013.06.040
更新日期:2013-08-01 00:00:00
abstract::The coactivator PGC-1alpha is a key regulator of mitochondrial biogenesis and respiration, mediating expression of several transcription factors required for these programs. Three new studies reveal that PGC-1alpha expression is downregulated in patients with Huntington's disease (HD) and in several animal models of t...
journal_title:Cell
pub_type: 评论,杂志文章,评审
doi:10.1016/j.cell.2006.10.023
更新日期:2006-11-03 00:00:00
abstract::Metastasis is the leading cause of cancer-associated death but has been difficult to study because it involves a series of rare, stochastic events. To capture these events, we developed a sensitive method to tag and track pancreatic epithelial cells in a mouse model of pancreatic cancer. Tagged cells invaded and enter...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2011.11.025
更新日期:2012-01-20 00:00:00
abstract::Mosquito-borne flaviviruses, including dengue virus (DENV) and Zika virus (ZIKV), are a growing public health concern. Systems-level analysis of how flaviviruses hijack cellular processes through virus-host protein-protein interactions (PPIs) provides information about their replication and pathogenic mechanisms. We u...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2018.11.028
更新日期:2018-12-13 00:00:00
abstract::Antibodies to DNA and chromatin drive autoimmunity in systemic lupus erythematosus (SLE). Null mutations and hypomorphic variants of the secreted deoxyribonuclease DNASE1L3 are linked to familial and sporadic SLE, respectively. We report that DNASE1L3-deficient mice rapidly develop autoantibodies to DNA and chromatin,...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2016.05.034
更新日期:2016-06-30 00:00:00
abstract::A key feature of plants (as opposed to animals) is their ability to establish new organs not only during embryogenesis, but also throughout their development. A master regulator of organ initiation in plants is the phytohormone auxin. Auxin acts locally as a morphogen and is directionally transported from cell to cell...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2007.08.040
更新日期:2007-09-21 00:00:00
abstract::Biomechanical stress is a major stimulus for cardiac hypertrophy and the transition to heart failure. By generating mice that harbor a ventricular restricted knockout of the gp130 cytokine receptor via Cre-IoxP-mediated recombination, we demonstrate a critical role for a gp130-dependent myocyte survival pathway in the...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(00)80729-1
更新日期:1999-04-16 00:00:00
abstract::PD-1 immune checkpoint blockade provides significant clinical benefits for melanoma patients. We analyzed the somatic mutanomes and transcriptomes of pretreatment melanoma biopsies to identify factors that may influence innate sensitivity or resistance to anti-PD-1 therapy. We find that overall high mutational loads a...
journal_title:Cell
pub_type: 评论,杂志文章
doi:10.1016/j.cell.2016.02.065
更新日期:2016-03-24 00:00:00
abstract::Tyrosine phosphorylation controls many cellular functions. Yet the three-part toolkit that regulates phosphotyrosine signaling-tyrosine kinases, phosphotyrosine phosphatases, and Src Homology 2 (SH2) domains-is a relatively new innovation. Genomic analyses reveal how this revolutionary signaling system may have origin...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2010.08.023
更新日期:2010-09-03 00:00:00
abstract::In this issue of Cell, Uderhardt et al. employed intravital two-photon microscopy to examine tissue-resident macrophage responses to sterile cellular injuries of variable size. They observed that while multi-cell "macrolesions" are characteristically pro-inflammatory, resident macrophages can "cloak" single-cell micro...
journal_title:Cell
pub_type: 评论,杂志文章
doi:10.1016/j.cell.2019.03.042
更新日期:2019-04-18 00:00:00
abstract::The ongoing pandemic of a new human coronavirus, SARS-CoV-2, has generated enormous global concern. We and others in China were involved in the initial genome sequencing of the virus. Herein, we describe what genomic data reveal about the emergence SARS-CoV-2 and discuss the gaps in our understanding of its origins. ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2020.03.035
更新日期:2020-04-16 00:00:00
abstract::X-linked recessive chondrodysplasia punctata (CDPX) is a congenital defect of bone and cartilage development characterized by aberrant bone mineralization, severe underdevelopment of nasal cartilage, and distal phalangeal hypoplasia. A virtually identical phenotype is observed in the warfarin embryopathy, which is due...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(95)90367-4
更新日期:1995-04-07 00:00:00
abstract::The immortal strand hypothesis posits that the propensity of stem cell compartments to give rise to cancer in later life can be minimized if stem cells, during the process of self-renewal, retain those DNA strands with the fewest mutations acquired during DNA replication. In this Essay, I explore evidence in support o...
journal_title:Cell
pub_type: 杂志文章,评审
doi:10.1016/j.cell.2007.06.019
更新日期:2007-06-29 00:00:00
abstract::Mammalian models of longevity are related primarily to caloric restriction and alterations in metabolism. We examined mice in which type 5 adenylyl cyclase (AC5) is knocked out (AC5 KO) and which are resistant to cardiac stress and have increased median lifespan of approximately 30%. AC5 KO mice are protected from red...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2007.05.038
更新日期:2007-07-27 00:00:00
abstract::Glial cells are essential components of the nervous system. In this issue, Singhvi et al. uncover cellular and molecular mechanisms through which C. elegans glia shape sensory neuron terminals and thus control animal thermosensing behaviors. ...
journal_title:Cell
pub_type: 评论,杂志文章
doi:10.1016/j.cell.2016.04.052
更新日期:2016-05-05 00:00:00
abstract::Three membrane microdomains can be identified on docked vacuoles: "outside" membrane, not in contact with other vacuoles, "boundary" membrane that contacts adjacent vacuoles, and "vertices," where boundary and outside membrane meet. In living cells and in vitro, vacuole fusion occurs at vertices rather than from a cen...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(02)00632-3
更新日期:2002-02-08 00:00:00
abstract::Sharply delineated domains of cell types arise in developing tissues under instruction of inductive signal (morphogen) gradients, which specify distinct cell fates at different signal levels. The translation of a morphogen gradient into discrete spatial domains relies on precise signal responses at stable cell positio...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2013.03.023
更新日期:2013-04-25 00:00:00
abstract::At the final step of homologous recombination, Holliday junction-containing joint molecules (JMs) are resolved to form crossover or noncrossover products. The enzymes responsible for JM resolution in vivo remain uncertain, but three distinct endonucleases capable of resolving JMs in vitro have been identified: Mus81-M...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2012.03.023
更新日期:2012-04-13 00:00:00
abstract::Advances in genomic technology have produced an explosion of new information about the genetic basis for human disease, fueling extraordinarily high expectations for improved treatments. This perspective will take brief stock of what genetics/genomics have brought to clinical practice to date and what we might expect ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2011.09.013
更新日期:2011-09-30 00:00:00
abstract::Wnt signaling plays a critical role in embryonic development, and genetic aberrations in this network have been broadly implicated in colorectal cancer. We find that the Wnt receptor Frizzled2 (Fzd2) and its ligands Wnt5a/b are elevated in metastatic liver, lung, colon, and breast cancer cell lines and in high-grade t...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2014.10.032
更新日期:2014-11-06 00:00:00
abstract::Viral pandemics, such as the one caused by SARS-CoV-2, pose an imminent threat to humanity. Because of its recent emergence, there is a paucity of information regarding viral behavior and host response following SARS-CoV-2 infection. Here we offer an in-depth analysis of the transcriptional response to SARS-CoV-2 comp...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2020.04.026
更新日期:2020-05-28 00:00:00
abstract::The linear extrachromosomal ribosomal DNA of Tetrahymena is generated from a single integrated copy during macronuclear development. The free ends of this extrachromosomal gene contain 20-70 tandem repeats of the hexanucleotide CCCCAA. We have determined the nucleotide sequence at the same (3') end of the single, inte...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(82)90417-2
更新日期:1982-11-01 00:00:00