Abstract：
:The TDS4 gene of S. cerevisiae was isolated as an allele-specific high copy suppressor of mutations within the basic region of the TATA-binding protein (TBP). The gene is essential for viability and encodes a 596 aa protein. The first 300 aa of the TDS4 protein exhibit significant sequence similarity to the RNA polymerase II transcription factor TFIIB. However, TDS4 is required for RNA polymerase III transcription in vivo and in vitro. Antibodies specific for TDS4 or TBP react with the TFIIIB complex, indicating that both proteins are components of the RNA polymerase III initiation complex. These findings suggest that the RNA polymerase II and III initiation mechanisms are extremely similar, and they explain how the TATA-binding protein can function in both systems.
journal_name
Celljournal_title
Cellauthors
Buratowski S,Zhou Hdoi
10.1016/0092-8674(92)90351-csubject
Has Abstractpub_date
1992-10-16 00:00:00pages
221-30issue
2eissn
0092-8674issn
1097-4172pii
0092-8674(92)90351-Cjournal_volume
71pub_type
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