Abstract：
:Activation of the transcription factor NF-kappaB by tumor necrosis factor (TNF) and interleukin-1 (IL-1) requires the NF-kappaB-inducing kinase (NIK). In a yeast two-hybrid screen for NIK-interacting proteins, we have identified a protein kinase previously known as CHUK. Overexpression of CHUK activates a NF-kappaB-dependent reporter gene. A catalytically inactive mutant of CHUK is a dominant-negative inhibitor of TNF-, IL-1-, TRAF-, and NIK-induced NF-kappaB activation. CHUK associates with the NF-kappaB inhibitory protein, IkappaB-alpha, in mammalian cells. CHUK specifically phosphorylates IkappaB-alpha on both serine 32 and serine 36, modifications that are required for targeted degradation of IkappaB-alpha via the ubiquitin-proteasome pathway. This phosphorylation of IkappaB-alpha is greatly enhanced by NIK costimulation. Thus, CHUK is a NIK-activated IkappaB-alpha kinase that links TNF- and IL-1-induced kinase cascades to NF-kappaB activation.
journal_name
Celljournal_title
Cellauthors
Régnier CH,Song HY,Gao X,Goeddel DV,Cao Z,Rothe Mdoi
10.1016/s0092-8674(00)80344-xsubject
Has Abstractpub_date
1997-07-25 00:00:00pages
373-83issue
2eissn
0092-8674issn
1097-4172pii
S0092-8674(00)80344-Xjournal_volume
90pub_type
杂志文章相关文献
CELL文献大全abstract：:In eukaryotes, a specialized pathway of mRNA degradation termed nonsense-mediated decay (NMD) functions in mRNA quality control by recognizing and degrading mRNAs with aberrant termination codons. We demonstrate that NMD in yeast targets premature termination codon (PTC)-containing mRNA to P-bodies. Upf1p is sufficien...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2006.04.037
更新日期：2006-06-16 00:00:00
abstract：:The textbook view of gene activation is that the rate-limiting step is the interaction of RNA polymerase II (Pol II) with the gene's promoter. However, studies in a variety of systems, including human embryonic stem cells and the early Drosophila embryo, have begun to challenge this view. There is increasing evidence ...
journal_title：Cell
pub_type： 杂志文章,评审
doi：10.1016/j.cell.2011.04.021
更新日期：2011-05-13 00:00:00
abstract：:Phosphoinositide 3-kinases (PI3-Ks) are an important emerging class of drug targets, but the unique roles of PI3-K isoforms remain poorly defined. We describe here an approach to pharmacologically interrogate the PI3-K family. A chemically diverse panel of PI3-K inhibitors was synthesized, and their target selectivity...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2006.03.035
更新日期：2006-05-19 00:00:00
abstract：:The rate of in vivo transcription from the E. coli tRNA and rRNA promoters depends on both cellular growth rate and aminoacid availability. To investigate the molecular mechanisms involved we determined the extent of interaction of RNA polymerase with the promoter of the tyrT stable RNA gene. We show that the enzyme c...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(83)90229-5
更新日期：1983-11-01 00:00:00
abstract：:Apoptosis induced by TNF-receptor I (TNFR1) is thought to proceed via recruitment of the adaptor FADD and caspase-8 to the receptor complex. TNFR1 signaling is also known to activate the transcription factor NF-kappa B and promote survival. The mechanism by which this decision between cell death and survival is arbitr...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(03)00521-x
更新日期：2003-07-25 00:00:00
abstract：:Dietary soluble fibers are fermented by gut bacteria into short-chain fatty acids (SCFA), which are considered broadly health-promoting. Accordingly, consumption of such fibers ameliorates metabolic syndrome. However, incorporating soluble fiber inulin, but not insoluble fiber, into a compositionally defined diet, ind...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2018.09.004
更新日期：2018-10-18 00:00:00
abstract：:For the faithful initiation of transcription of exogenously added fibroin gene, a crude cellular extract was prepared from the posterior silk glands where fibroin gene is specifically transcribed. The faithful initiation was demonstrated by the assay with use of truncated fibroin gene templates and confirmed by nuclea...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(81)90371-8
更新日期：1981-11-01 00:00:00
abstract：:Muscle cell cultures from Drosophila melanogaster were obtained by plating dissociated gastrula stage embryo cells on protamine-treated culture dishes. They myogenic cells in these cultures fuse to form multinucleated pulsating cells by 15 hr after plating. An analysis of protein synthesis during myogenesis in these c...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(78)90210-6
更新日期：1978-04-01 00:00:00
abstract：:As in eukaryotes, bacterial genomes are not randomly folded. Bacterial genetic information is generally carried on a circular chromosome with a single origin of replication from which two replication forks proceed bidirectionally toward the opposite terminus region. Here, we investigate the higher-order architecture o...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2017.12.027
更新日期：2018-02-08 00:00:00
abstract：:Human autoantibodies that recognize the spindle poles of mammals, plants, and insects were found to recognize two antigens in yeast. One of these proteins, called SPA1 (for Spindle Pole Antigen), is antigenically related to the spindle poles of a diverse set of organisms. The gene encoding SPA1 was cloned by immunoscr...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(88)90977-4
更新日期：1988-09-09 00:00:00
abstract：:Ca(2+) binding to synaptotagmin 1 triggers fast exocytosis of synaptic vesicles that have been primed for release by SNARE-complex assembly. Besides synaptotagmin 1, fast Ca(2+)-triggered exocytosis requires complexins. Synaptotagmin 1 and complexins both bind to assembled SNARE complexes, but it is unclear how their ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2006.08.030
更新日期：2006-09-22 00:00:00
abstract：:The crystal structure of the snake toxin fasciculin, bound to mouse acetylcholinesterase (mAChE), at 3.2 A resolution reveals a synergistic three-point anchorage consistent with the picomolar dissociation constant of the complex. Loop II of fasciculin contains a cluster of hydrophobic residues that interact with the p...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(95)90128-0
更新日期：1995-11-03 00:00:00
abstract：:Evidence is now mounting that liquid-liquid phase separation (LLPS) underlies the formation of membraneless compartments in cells. This realization has motivated major efforts to delineate the function of such biomolecular condensates in normal cells and their roles in contexts ranging from development to age-related ...
journal_title：Cell
pub_type： 杂志文章,评审
doi：10.1016/j.cell.2018.12.035
更新日期：2019-01-24 00:00:00
abstract：:In nematodes, a fraction of mRNAs contains a common 22 nucleotide 5' terminal spliced leader (SL) sequence derived by trans splicing. Here, we show that a cell-free extract prepared from developing embryos of the parasitic nematode Ascaris lumbricoides catalyzes accurate and efficient SL addition to a synthetic pre-mR...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(90)90689-c
更新日期：1990-06-29 00:00:00
abstract：:Independently derived mutants of Chinese hamster ovary cells have been isolated and shown to exhibit a subtle glycosylation defect resulting in the premature termination of certain asparagine-linked carbohydrate moieties. This carbohydrate alteration is akin to the types of structural variation termed microheterogenei...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(81)90440-2
更新日期：1981-03-01 00:00:00
abstract：:We have used liposomes to deliver DNAase I inside normal Syrian hamster embryo (SHE) cells. We showed the entrance of DNAase I inside the cell by dose-dependent cytotoxicity; and the entrance of DNAase I into the nucleus by the induction of chromosomal aberrations and somatic mutation at the HPRT locus (but not at the...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(84)90450-1
更新日期：1984-12-01 00:00:00
abstract：:Cells are protected from endoplasmic reticulum stress through the unfolded protein response (UPR). In this issue of Cell, Schinzel, Higuchi-Sanabria, Shalem et al., identify a mechanism that helps cells cope with ER stress but is independent of canonical UPR activation, instead involving the extracellular matrix hyalu...
journal_title：Cell
pub_type： 评论,杂志文章
doi：10.1016/j.cell.2019.11.012
更新日期：2019-11-27 00:00:00
abstract：:Suppressor of Hairless (Su(H)) is required at two stages of adult sensory organ development in Drosophila. Complete loss of Su(H) function results in a "neurogenic" phenotype in imaginal discs, in which too many cells adopt the sensory organ precursor cell fate. Su(H) is also involved in controlling the fates of sensi...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(92)90641-o
更新日期：1992-06-26 00:00:00
abstract：:Transposons evolve rapidly and can mobilize and trigger genetic instability. Piwi-interacting RNAs (piRNAs) silence these genome pathogens, but it is unclear how the piRNA pathway adapts to invasion of new transposons. In Drosophila, piRNAs are encoded by heterochromatic clusters and maternally deposited in the embryo...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2011.11.042
更新日期：2011-12-23 00:00:00
abstract：:The precise control of miR-17∼92 microRNA (miRNA) is essential for normal development, and overexpression of certain miRNAs from this cluster is oncogenic. Here, we find that the relative expression of the six miRNAs processed from the primary (pri-miR-17∼92) transcript is dynamically regulated during embryonic stem c...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2015.07.008
更新日期：2015-08-13 00:00:00
abstract：:Activating mutations of FLT3 occur in about 30% of acute myeloid leukemia (AML) cases and are associated with relapse and poor prognosis. Midostaurin is the first drug approved for AML since 2000, and the first multi-kinase inhibitor approved for the FLT3-mutant subtype. To view this Bench to Bedside, open or download...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2017.11.007
更新日期：2017-11-16 00:00:00
abstract：:Cancer cell of origin is difficult to identify by analyzing cells within terminal stage tumors, whose identity could be concealed by the acquired plasticity. Thus, an ideal approach to identify the cell of origin is to analyze proliferative abnormalities in distinct lineages prior to malignancy. Here, we use mosaic an...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2011.06.014
更新日期：2011-07-22 00:00:00
abstract：:We describe a new repressible binary expression system based on the regulatory genes from the Neurospora qa gene cluster. This "Q system" offers attractive features for transgene expression in Drosophila and mammalian cells: low basal expression in the absence of the transcriptional activator QF, high QF-induced expre...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2010.02.025
更新日期：2010-04-30 00:00:00
abstract：:Through dissecting the link between spatial genome organization and DNA replication timing, Sima et al. (2018) discover early replicating control elements (ERCEs), a new type of cis-acting elements that regulate replication timing, transcription, and multiple layers of three-dimensional features of genome organization...
journal_title：Cell
pub_type： 评论,杂志文章
doi：10.1016/j.cell.2019.01.027
更新日期：2019-02-07 00:00:00
abstract：:The sequences corresponding to the 18S and 28S rRNAs have been mapped within a cloned 17 kilobase (kb) fragment formed by Eco R1 cleavage of Drosophila melanogaster rDNA. This fragment, Dm103, represents the longer of two major types of repeating units that are present in the rDNA of this fly, and was cloned as a hybr...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(77)90212-4
更新日期：1977-02-01 00:00:00
abstract：:How metazoan mechanotransduction channels sense mechanical stimuli is not well understood. The NOMPC channel in the transient receptor potential (TRP) family, a mechanotransduction channel for Drosophila touch sensation and hearing, contains 29 Ankyrin repeats (ARs) that associate with microtubules. These ARs have bee...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2015.08.024
更新日期：2015-09-10 00:00:00
abstract：:Vg1 is a TGF beta-related growth factor encoded by a maternal mRNA localized to vegetal blastomeres in Xenopus embryos. Vg1 precursor protein is abundant in vegetal cells, but the processed mature form has not been readily detected and no activity has been demonstrated for the putative Vg1 mature protein. We have engi...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(93)80045-g
更新日期：1993-08-13 00:00:00
abstract：:The intermediate filament proteins, desmin and vimentin, are phosphorylated in skeletal muscle cells in vivo. Desmin kinase activities have been purified from mature chicken skeletal muscle and identified as the cAMP-dependent kinases. Chicken skeletal muscle contains two cAMP-dependent protein kinases which are simil...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(81)90278-6
更新日期：1981-01-01 00:00:00
abstract：:SecA, the peripheral subunit of E. coli preprotein translocase, alternates between a membrane inserted and a deinserted state as part of the catalytic cycle of preprotein translocation. When SecA is complexed with SecY/E and preprotein, ATP drives a profound conformational change, leading to membrane insertion of a 30...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(94)90582-7
更新日期：1994-09-09 00:00:00
abstract：:The p53 tumor-suppressor protein prevents cancer development through various mechanisms, including the induction of cell-cycle arrest, apoptosis, and the maintenance of genome stability. We have identified a p53-inducible gene named TIGAR (TP53-induced glycolysis and apoptosis regulator). TIGAR expression lowered fruc...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2006.05.036
更新日期：2006-07-14 00:00:00
