Abstract：
:How metazoan mechanotransduction channels sense mechanical stimuli is not well understood. The NOMPC channel in the transient receptor potential (TRP) family, a mechanotransduction channel for Drosophila touch sensation and hearing, contains 29 Ankyrin repeats (ARs) that associate with microtubules. These ARs have been postulated to act as a tether that conveys force to the channel. Here, we report that these N-terminal ARs form a cytoplasmic domain essential for NOMPC mechanogating in vitro, mechanosensitivity of touch receptor neurons in vivo, and touch-induced behaviors of Drosophila larvae. Duplicating the ARs elongates the filaments that tether NOMPC to microtubules in mechanosensory neurons. Moreover, microtubule association is required for NOMPC mechanogating. Importantly, transferring the NOMPC ARs to mechanoinsensitive voltage-gated potassium channels confers mechanosensitivity to the chimeric channels. These experiments strongly support a tether mechanism of mechanogating for the NOMPC channel, providing insights into the basis of mechanosensitivity of mechanotransduction channels.
journal_name
Celljournal_title
Cellauthors
Zhang W,Cheng LE,Kittelmann M,Li J,Petkovic M,Cheng T,Jin P,Guo Z,Göpfert MC,Jan LY,Jan YNdoi
10.1016/j.cell.2015.08.024subject
Has Abstractpub_date
2015-09-10 00:00:00pages
1391-403issue
6eissn
0092-8674issn
1097-4172pii
S0092-8674(15)01037-5journal_volume
162pub_type
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