Abstract:
:We have used confocal videomicroscopy in real time to delineate the adhesive interactions supporting platelet thrombus formation on biologically relevant surfaces. Type I collagen fibrils exposed to flowing blood adsorb von Willebrand factor (vWF), to which platelets become initially tethered with continuous surface translocation mediated by the membrane glycoprotein Ib alpha. This step is essential at high wall shear rates to allow subsequent irreversible adhesion and thrombus growth mediated by the integrins alpha2beta1 and alpha(IIb)beta3. On subendothelial matrix, endogenous vWF and adsorbed plasma vWF synergistically initiate platelet recruitment, and alpha2beta1 remains key along with alpha(IIb)beta3 for normal thrombus development at all but low shear rates. Thus, hemodynamic forces and substrate characteristics define the platelet adhesion pathways leading to thrombogenesis.
journal_name
Celljournal_title
Cellauthors
Savage B,Almus-Jacobs F,Ruggeri ZMdoi
10.1016/s0092-8674(00)81607-4subject
Has Abstractpub_date
1998-09-04 00:00:00pages
657-66issue
5eissn
0092-8674issn
1097-4172pii
S0092-8674(00)81607-4journal_volume
94pub_type
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