Abstract：
:Vaccinia virus recombinants were generated which express the intact gp70/p15E of Friend mink cell focus inducing virus (F-MCFV) or truncated forms of the glycoprotein that lack the transmembrane and cytoplasmic domains. The transport of the intact and truncated envelope glycoproteins to apical or basolateral surfaces was studied in the polarized epithelial MDCK cell line. Infection of MDCK cells with the recombinant expressing the intact F-MCFV envelope glycoprotein resulted in transport exclusively to the basolateral surfaces, whereas the recombinant expressing the truncated glycoprotein was found to be secreted from both the apical and basolateral surfaces. Thus removal of the transmembrane and cytoplasmic domains of the p15E protein results in a loss of directional transport to the basolateral membrane of polarized epithelial cells.
journal_name
Celljournal_title
Cellauthors
Stephens EB,Compans RWdoi
10.1016/0092-8674(86)90820-2subject
Has Abstractpub_date
1986-12-26 00:00:00pages
1053-9issue
6eissn
0092-8674issn
1097-4172pii
0092-8674(86)90820-2journal_volume
47pub_type
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