Abstract：
:The contribution of CD4+ T cells to protective or pathogenic immune responses to SARS-CoV-2 infection remains unknown. Here, we present single-cell transcriptomic analysis of >100,000 viral antigen-reactive CD4+ T cells from 40 COVID-19 patients. In hospitalized patients compared to non-hospitalized patients, we found increased proportions of cytotoxic follicular helper cells and cytotoxic T helper (TH) cells (CD4-CTLs) responding to SARS-CoV-2 and reduced proportion of SARS-CoV-2-reactive regulatory T cells (TREG). Importantly, in hospitalized COVID-19 patients, a strong cytotoxic TFH response was observed early in the illness, which correlated negatively with antibody levels to SARS-CoV-2 spike protein. Polyfunctional TH1 and TH17 cell subsets were underrepresented in the repertoire of SARS-CoV-2-reactive CD4+ T cells compared to influenza-reactive CD4+ T cells. Together, our analyses provide insights into the gene expression patterns of SARS-CoV-2-reactive CD4+ T cells in distinct disease severities.
journal_name
Celljournal_title
Cellauthors
Meckiff BJ,Ramírez-Suástegui C,Fajardo V,Chee SJ,Kusnadi A,Simon H,Eschweiler S,Grifoni A,Pelosi E,Weiskopf D,Sette A,Ay F,Seumois G,Ottensmeier CH,Vijayanand Pdoi
10.1016/j.cell.2020.10.001subject
Has Abstractpub_date
2020-11-25 00:00:00pages
1340-1353.e16issue
5eissn
0092-8674issn
1097-4172pii
S0092-8674(20)31307-6journal_volume
183pub_type
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