Abstract：
:Although rational method and global technique have been successfully applied in strain improvement respectively, the demand for engineering complex phenotypes required combinatorial approach. The technology of genome shuffling has been presented as a novel whole genome engineering approach for the rapid improvement of cellular phenotypes. This approach using recursive protoplast fusion with multi-parental strains offers the advantage of recombination throughout the entire genome without the necessity for genome sequence data or network information. Genome shuffling has been demonstrated as an effective method, which is not only for producing improved strain but also for providing information on complex phenotype. In this review we attempt to present the advantage of genome shuffling, introduce the procedure of this technology, summarize the applications of this approach for phenotype improvement and then give perspective on the development of this method in the future.
journal_name
Biotechnol Advjournal_title
Biotechnology advancesauthors
Gong J,Zheng H,Wu Z,Chen T,Zhao Xdoi
10.1016/j.biotechadv.2009.05.016subject
Has Abstractpub_date
2009-11-01 00:00:00pages
996-1005issue
6eissn
0734-9750issn
1873-1899pii
S0734-9750(09)00108-6journal_volume
27pub_type
杂志文章,评审abstract：:Quantitative real-time PCR (qPCR) has been widely used in recent environmental microbial ecology studies as a tool for detecting and quantifying microorganisms of interest, which aids in better understandings of the complexity of wastewater microbial communities. Although qPCR can be used to provide more specific and ...
journal_title：Biotechnology advances
pub_type： 杂志文章,评审
doi：10.1016/j.biotechadv.2013.05.010
更新日期：2013-12-01 00:00:00
abstract：:Grassland is of major importance for agricultural production and provides valuable ecosystem services. Its impact is likely to rise in changing socio-economic and climatic environments. High yielding forage grass species are major components of sustainable grassland production. Understanding the genome structure and f...
journal_title：Biotechnology advances
pub_type： 杂志文章,评审
doi：10.1016/j.biotechadv.2013.11.010
更新日期：2014-01-01 00:00:00
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journal_title：Biotechnology advances
pub_type： 杂志文章
doi：10.1016/0734-9750(92)91351-e
更新日期：1992-01-01 00:00:00
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journal_title：Biotechnology advances
pub_type： 杂志文章,评审
doi：10.1016/j.biotechadv.2020.107537
更新日期：2020-01-01 00:00:00
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journal_title：Biotechnology advances
pub_type： 杂志文章,评审
doi：10.1016/j.biotechadv.2019.06.011
更新日期：2019-11-15 00:00:00
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journal_title：Biotechnology advances
pub_type： 杂志文章,评审
doi：10.1016/j.biotechadv.2009.08.005
更新日期：2010-01-01 00:00:00
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journal_title：Biotechnology advances
pub_type： 杂志文章,评审
doi：10.1016/j.biotechadv.2003.08.005
更新日期：2003-12-01 00:00:00
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journal_title：Biotechnology advances
pub_type： 杂志文章
doi：10.1016/s0734-9750(00)00041-0
更新日期：2000-08-01 00:00:00
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journal_title：Biotechnology advances
pub_type： 杂志文章,评审
doi：10.1016/j.biotechadv.2016.11.001
更新日期：2017-01-01 00:00:00
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journal_title：Biotechnology advances
pub_type： 杂志文章
doi：10.1016/0734-9750(89)90357-1
更新日期：1989-01-01 00:00:00
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journal_title：Biotechnology advances
pub_type： 杂志文章,评审
doi：10.1016/j.biotechadv.2017.10.001
更新日期：2018-01-01 00:00:00
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journal_title：Biotechnology advances
pub_type： 杂志文章,评审
doi：10.1016/j.biotechadv.2009.07.002
更新日期：2009-11-01 00:00:00
abstract：:Additive manufacturing or 3D printing has spearheaded a revolution in the biomedical sector allowing the rapid prototyping of medical devices. The recent advancements in bioprinting technology are enabling the development of potential new therapeutic options with respect to tissue engineering and regenerative medicine...
journal_title：Biotechnology advances
pub_type： 杂志文章,评审
doi：10.1016/j.biotechadv.2019.107448
更新日期：2019-12-01 00:00:00
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journal_title：Biotechnology advances
pub_type： 杂志文章,评审
doi：10.1016/j.biotechadv.2019.05.001
更新日期：2019-11-01 00:00:00
abstract：:Developments in plant genetic engineering technology will shortly permit the commercial introduction of transgenic crop varieties resistant to a number of non-selective herbicides. High levels of tolerance have been achieved both by overexpression of a target protein and by modification of that target to an insensitiv...
journal_title：Biotechnology advances
pub_type： 杂志文章
doi：10.1016/0734-9750(90)91068-r
更新日期：1990-01-01 00:00:00
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journal_title：Biotechnology advances
pub_type： 杂志文章,评审
doi：10.1016/j.biotechadv.2015.07.006
更新日期：2015-12-01 00:00:00
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journal_title：Biotechnology advances
pub_type： 杂志文章,评审
doi：10.1016/j.biotechadv.2012.03.006
更新日期：2012-11-01 00:00:00
abstract：:The ability to use progenitor cells for regenerative medicine remains an evolving but elusive clinical goal. A serious obstacle towards widespread use of stem cells for tissue regeneration is the challenges that face these cells when they are placed in vivo into a wound for therapy. These environments are hypoxic, aci...
journal_title：Biotechnology advances
pub_type： 杂志文章,评审
doi：10.1016/j.biotechadv.2012.11.003
更新日期：2013-09-01 00:00:00
abstract：:Cyclodextrins (CDs) are a family of cyclic oligosaccharides that are composed of alpha-1,4-linked glucopyranose subunits. Cyclodextrins are produced from starch by enzymatic degradation. These macrocyclic carbohydrates with apolar internal cavities can form complexes with and solubilize many normally water-insoluble c...
journal_title：Biotechnology advances
pub_type： 杂志文章
doi：10.1016/s0734-9750(02)00020-4
更新日期：2002-12-01 00:00:00
abstract：:Tissue maceration was generally elucidated by the action of endo-polygalacturonase and endo-pectate or -pectin lyase (endo-PAL or -PNL). In a process of screening of Erwinia and Pseudomonas strains for enzymatic pulping of pectocellulosic bast fibers, it was found that their PAL productivity was not completely related...
journal_title：Biotechnology advances
pub_type： 杂志文章
doi：10.1016/0734-9750(88)90572-1
更新日期：1988-01-01 00:00:00
abstract：:Within five years, the CRISPR-Cas system has emerged as the dominating tool for genome engineering, while also changing the speed and efficiency of metabolic engineering in conventional (Saccharomyces cerevisiae and Schizosaccharomyces pombe) and non-conventional (Yarrowia lipolytica, Pichia pastoris syn. Komagataella...
journal_title：Biotechnology advances
pub_type： 杂志文章,评审
doi：10.1016/j.biotechadv.2018.01.006
更新日期：2018-01-01 00:00:00
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journal_title：Biotechnology advances
pub_type： 杂志文章,评审
doi：10.1016/j.biotechadv.2019.04.002
更新日期：2019-01-01 00:00:00
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journal_title：Biotechnology advances
pub_type： 杂志文章,评审
doi：10.1016/j.biotechadv.2011.04.001
更新日期：2011-11-01 00:00:00
abstract：:The impact of additive manufacturing in our lives has been increasing constantly. One of the frontiers in this change is the medical devices. 3D printing technologies not only enable the personalization of implantable devices with respect to patient-specific anatomy, pathology and biomechanical properties but they als...
journal_title：Biotechnology advances
pub_type： 杂志文章,评审
doi：10.1016/j.biotechadv.2018.02.004
更新日期：2018-01-01 00:00:00
abstract：:Lactoferrin, the main iron-binding protein of milk, has biological activities that are essential for the newborn and are beneficial for adults. Given this beneficial effect, there is broad interest in exogenous sources of lactoferrin in human nutrition. Consequently, several transgenic approaches to produce lactoferri...
journal_title：Biotechnology advances
pub_type： 杂志文章
doi：10.1016/j.biotechadv.2010.07.002
更新日期：2010-11-01 00:00:00
abstract：:Plant cell suspension culture (PCSC) has emerged as a viable technology to produce plant specialized metabolites (PSM). While Taxol® and ginsenoside are two examples of successfully commercialized PCSC-derived PSM, widespread utilization of the PCSC platform has yet to be realized primarily due to a lack of understand...
journal_title：Biotechnology advances
pub_type： 杂志文章,评审
doi：10.1016/j.biotechadv.2020.107635
更新日期：2020-12-01 00:00:00
abstract：:Bovine chymosin, an aspartyl protease extracted from abomasum of suckling calves, is synthesized in vivo as preprochymosin and secreted as prochymosin which is autocatalytically activated to chymosin. Chymosin is bilobular, with Asp 32 and Asp 215 acting as the catalytic residues. Chymosin A and chymosin B have pH opt...
journal_title：Biotechnology advances
pub_type： 杂志文章
doi：10.1016/s0734-9750(99)00010-5
更新日期：1999-09-01 00:00:00
abstract：:Differentiation of cancer cells entails the reversion of phenotype from malignant to the original. The conversion to cell type characteristic for another tissue is named transdifferentiation. Differentiation/transdifferentiation of malignant cells in high grade tumor mass could serve as a nonaggressive approach that p...
journal_title：Biotechnology advances
pub_type： 杂志文章,评审
doi：10.1016/j.biotechadv.2018.04.001
更新日期：2018-11-01 00:00:00
abstract：:In the biological milieu of a cell, soluble crowding molecules and rigid confined environments strongly influence whether the protein is properly folded, intrinsically disordered proteins assemble into distinct phases, or a denatured or aggregated protein species is favored. Such crowding and confinement factors act t...
journal_title：Biotechnology advances
pub_type： 杂志文章,评审
doi：10.1016/j.biotechadv.2020.107573
更新日期：2020-01-01 00:00:00
abstract：:Osteochondral defect management and repair remain a significant challenge in orthopedic surgery. Osteochondral defects contain damage to both the articular cartilage as well as the underlying subchondral bone. In order to repair an osteochondral defect the needs of the bone, cartilage and the bone-cartilage interface ...
journal_title：Biotechnology advances
pub_type： 杂志文章,评审
doi：10.1016/j.biotechadv.2012.11.004
更新日期：2013-09-01 00:00:00
