Protein folding and assembly in confined environments: Implications for protein aggregation in hydrogels and tissues.

Abstract:

:In the biological milieu of a cell, soluble crowding molecules and rigid confined environments strongly influence whether the protein is properly folded, intrinsically disordered proteins assemble into distinct phases, or a denatured or aggregated protein species is favored. Such crowding and confinement factors act to exclude solvent volume from the protein molecules, resulting in an increased local protein concentration and decreased protein entropy. A protein's structure is inherently tied to its function. Examples of processes where crowding and confinement may strongly influence protein function include transmembrane protein dimerization, enzymatic activity, assembly of supramolecular structures (e.g., microtubules), nuclear condensates containing transcriptional machinery, protein aggregation in the contexts of disease and protein therapeutics. Historically, most protein structures have been determined from pure, dilute protein solutions or pure crystals. However, these are not the environments in which these proteins function. Thus, there has been an increased emphasis on analyzing protein structure and dynamics in more "in vivo-like" environments. Complex in vitro models using hydrogel scaffolds to study proteins may better mimic features of the in vivo environment. Therefore, analytical techniques need to be optimized for real-time analysis of proteins within hydrogel scaffolds.

journal_name

Biotechnol Adv

journal_title

Biotechnology advances

authors

Simpson LW,Good TA,Leach JB

doi

10.1016/j.biotechadv.2020.107573

subject

Has Abstract

pub_date

2020-01-01 00:00:00

pages

107573

eissn

0734-9750

issn

1873-1899

pii

S0734-9750(20)30070-7

journal_volume

42

pub_type

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