Abstract：
:Industrial biotechnology is reliant on native pathway engineering or foreign pathway introduction for efficient biosynthesis of target products. Chromosomal integration, with intrinsic genetic stability, is an indispensable step for reliable expression of homologous or heterologous genes and pathways in large-scale and long-term fermentation. With advances in synthetic biology and CRISPR-based genome editing approaches, a wide variety of novel enabling technologies have been developed for single-step, markerless, multi-locus genomic integration of large biochemical pathways, which significantly facilitate microbial overproduction of chemicals, pharmaceuticals and other value-added biomolecules. Notably, the newly discovered homology-mediated end joining strategy could be widely applicable for high-efficiency genomic integration in a number of homologous recombination-deficient microbes. In this review, we explore the fundamental principles and characteristics of genomic integration, and highlight the development and applications of targeted integration approaches in the three representative industrial microbial systems, including Escherichia coli, actinomycetes and yeasts.
journal_name
Biotechnol Advjournal_title
Biotechnology advancesauthors
Li L,Liu X,Wei K,Lu Y,Jiang Wdoi
10.1016/j.biotechadv.2019.04.002subject
Has Abstractpub_date
2019-01-01 00:00:00pages
730-745issue
5eissn
0734-9750issn
1873-1899pii
S0734-9750(19)30061-8journal_volume
37pub_type
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