Abstract:
:Plant cell suspension culture (PCSC) has emerged as a viable technology to produce plant specialized metabolites (PSM). While Taxol® and ginsenoside are two examples of successfully commercialized PCSC-derived PSM, widespread utilization of the PCSC platform has yet to be realized primarily due to a lack of understanding of the molecular genetics of PSM biosynthesis. Recent advances in computational, molecular and synthetic biology tools provide the opportunity to rapidly characterize and harness the specialized metabolic potential of plants. Here, we discuss the prospects of integrating computational modeling, artificial intelligence, and precision genome editing (CRISPR/Cas and its variants) toolboxes to discover the genetic regulators of PSM. We also explore how synthetic biology can be applied to develop metabolically optimized PSM-producing native and heterologous PCSC systems. Taken together, this review provides an interdisciplinary approach to realize and link the potential of next-generation computational and molecular tools to convert PCSC into commercially viable PSM-producing biofactories.
journal_name
Biotechnol Advjournal_title
Biotechnology advancesauthors
Arya SS,Rookes JE,Cahill DM,Lenka SKdoi
10.1016/j.biotechadv.2020.107635subject
Has Abstractpub_date
2020-12-01 00:00:00pages
107635eissn
0734-9750issn
1873-1899pii
S0734-9750(20)30137-3journal_volume
45pub_type
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