Abstract：
:An increasing number of proteins are currently available on the market as therapeutics and this branch of the pharmaceutical industry will expand substantially during the coming years. As many diseases result from dysfunction of proteins forming multicomponent complexes, protein drugs with their inherent high specificity and affinity seem to be optimal medical agents. On the other hand, proteins are often highly instable and sensitive to degradation, which questions their applicability as effective therapeutics. Therefore, redesign and engineering of proteins is usually a required step in the present day drug development. Several approaches have been applied to optimize the protein properties central to their pharmaceutical use. This review focuses on different strategies that improve two crucial factors influencing protein drug efficiency: protein stability and its in vivo half-life. We provide examples of successful genetic and chemical modifications applied in the design of effective protein therapeutics.
journal_name
Biotechnol Advjournal_title
Biotechnology advancesauthors
Szlachcic A,Zakrzewska M,Otlewski Jdoi
10.1016/j.biotechadv.2011.03.005subject
Has Abstractpub_date
2011-07-01 00:00:00pages
436-41issue
4eissn
0734-9750issn
1873-1899pii
S0734-9750(11)00038-3journal_volume
29pub_type
杂志文章,评审abstract：:Cell-free protein synthesis systems enable the direct in vitro expression of proteins from template DNA or RNA. Use of biochemical and bioengineering techniques has greatly improved the yields and productivities of cell-free systems. In some cases, the yields approach the in vivo levels. Moreover, in vitro systems are...
journal_title：Biotechnology advances
pub_type： 杂志文章
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journal_title：Biotechnology advances
pub_type： 杂志文章,评审
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journal_title：Biotechnology advances
pub_type： 杂志文章,评审
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更新日期：2021-01-01 00:00:00
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journal_title：Biotechnology advances
pub_type： 杂志文章,评审
doi：10.1016/j.biotechadv.2018.01.006
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journal_title：Biotechnology advances
pub_type： 杂志文章,评审
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journal_title：Biotechnology advances
pub_type： 杂志文章
doi：10.1016/s0734-9750(99)00010-5
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journal_title：Biotechnology advances
pub_type： 杂志文章,评审
doi：10.1016/j.biotechadv.2018.11.011
更新日期：2020-01-01 00:00:00
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journal_title：Biotechnology advances
pub_type： 杂志文章,评审
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journal_title：Biotechnology advances
pub_type： 杂志文章,评审
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journal_title：Biotechnology advances
pub_type： 杂志文章
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journal_title：Biotechnology advances
pub_type： 杂志文章
doi：10.1016/j.biotechadv.2012.01.016
更新日期：2012-07-01 00:00:00
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journal_title：Biotechnology advances
pub_type： 杂志文章,评审
doi：10.1016/j.biotechadv.2013.05.005
更新日期：2013-12-01 00:00:00
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journal_title：Biotechnology advances
pub_type： 杂志文章,评审
doi：10.1016/j.biotechadv.2015.03.001
更新日期：2015-11-01 00:00:00
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journal_title：Biotechnology advances
pub_type： 杂志文章,评审
doi：10.1016/j.biotechadv.2013.02.010
更新日期：2013-11-01 00:00:00
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journal_title：Biotechnology advances
pub_type： 杂志文章
doi：10.1016/0734-9750(92)91351-e
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journal_title：Biotechnology advances
pub_type： 杂志文章,评审
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journal_title：Biotechnology advances
pub_type： 杂志文章,评审
doi：10.1016/j.biotechadv.2015.08.001
更新日期：2015-12-01 00:00:00
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journal_title：Biotechnology advances
pub_type： 杂志文章,评审
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journal_title：Biotechnology advances
pub_type： 杂志文章,评审
doi：10.1016/j.biotechadv.2015.03.002
更新日期：2015-11-01 00:00:00
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journal_title：Biotechnology advances
pub_type： 杂志文章,评审
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更新日期：2008-11-01 00:00:00
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journal_title：Biotechnology advances
pub_type： 杂志文章,评审
doi：10.1016/j.biotechadv.2015.05.008
更新日期：2015-11-01 00:00:00
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journal_title：Biotechnology advances
pub_type： 杂志文章,评审
doi：10.1016/j.biotechadv.2013.05.010
更新日期：2013-12-01 00:00:00
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journal_title：Biotechnology advances
pub_type： 杂志文章,评审
doi：10.1016/j.biotechadv.2014.12.009
更新日期：2015-09-01 00:00:00
abstract：:The ubiquitous presence of allergens in the human food supply coupled with increased awareness of food allergies warrants undertaking appropriate preventive measures to protect sensitive consumers from unwanted exposure to offending food allergens. Attempts to reduce or eliminate food allergenicity through food proces...
journal_title：Biotechnology advances
pub_type： 杂志文章,评审
doi：10.1016/j.biotechadv.2005.05.008
更新日期：2005-09-01 00:00:00
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journal_title：Biotechnology advances
pub_type： 杂志文章
doi：10.1016/0734-9750(94)90023-x
更新日期：1994-01-01 00:00:00
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journal_title：Biotechnology advances
pub_type： 杂志文章,评审
doi：10.1016/j.biotechadv.2018.04.001
更新日期：2018-11-01 00:00:00
abstract：:The transition from the vegetative to reproductive stage followed by inflorescence is a critical step in plant life; therefore, studies of the genes that influence flowering time have always been of great interest to scientists. Flowering is a process controlled by many genes interacting mutually in a genetic network,...
journal_title：Biotechnology advances
pub_type： 杂志文章,评审
doi：10.1016/j.biotechadv.2013.09.008
更新日期：2014-01-01 00:00:00
abstract：:The biotech industry is, nowadays, facing unparalleled challenges due to the enhanced demand for biotechnology-based human therapeutic products, such as monoclonal antibodies (mAbs). This has led companies to improve substantially their upstream processes, with the yield of monoclonals increasing to titers never seen ...
journal_title：Biotechnology advances
pub_type： 杂志文章,评审
doi：10.1016/j.biotechadv.2011.03.006
更新日期：2011-11-01 00:00:00
