Abstract：
:Within five years, the CRISPR-Cas system has emerged as the dominating tool for genome engineering, while also changing the speed and efficiency of metabolic engineering in conventional (Saccharomyces cerevisiae and Schizosaccharomyces pombe) and non-conventional (Yarrowia lipolytica, Pichia pastoris syn. Komagataella phaffii, Kluyveromyces lactis, Candida albicans and C. glabrata) yeasts. Especially in S. cerevisiae, an extensive toolbox of advanced CRISPR-related applications has been established, including crisprTFs and gene drives. The comparison of innovative CRISPR-Cas expression strategies in yeasts presented here may also serve as guideline to implement and refine CRISPR-Cas systems for highly efficient genome editing in other eukaryotic organisms.
journal_name
Biotechnol Advjournal_title
Biotechnology advancesauthors
Raschmanová H,Weninger A,Glieder A,Kovar K,Vogl Tdoi
10.1016/j.biotechadv.2018.01.006subject
Has Abstractpub_date
2018-01-01 00:00:00pages
641-665issue
3eissn
0734-9750issn
1873-1899pii
S0734-9750(18)30006-5journal_volume
36pub_type
杂志文章,评审abstract：:The high plasticity of the active-site cavity of cytochromes P450, permitting reactivity toward a vast array of compounds, makes these enzymes attractive targets for biotechnological application. Escalating attention in this area is driven by remarkable progress in the rational design by DNA shuffling of self-sufficie...
journal_title：Biotechnology advances
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journal_title：Biotechnology advances
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journal_title：Biotechnology advances
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journal_title：Biotechnology advances
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journal_title：Biotechnology advances
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journal_title：Biotechnology advances
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journal_title：Biotechnology advances
pub_type： 杂志文章,评审
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journal_title：Biotechnology advances
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journal_title：Biotechnology advances
pub_type： 杂志文章,评审
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journal_title：Biotechnology advances
pub_type： 杂志文章
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更新日期：1994-01-01 00:00:00
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journal_title：Biotechnology advances
pub_type： 杂志文章
doi：10.1016/j.biotechadv.2009.04.016
更新日期：2009-09-01 00:00:00
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journal_title：Biotechnology advances
pub_type： 杂志文章,评审
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更新日期：2016-11-01 00:00:00
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journal_title：Biotechnology advances
pub_type： 杂志文章,评审
doi：10.1016/j.biotechadv.2005.05.008
更新日期：2005-09-01 00:00:00
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journal_title：Biotechnology advances
pub_type： 杂志文章,评审
doi：10.1016/j.biotechadv.2018.04.001
更新日期：2018-11-01 00:00:00
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journal_title：Biotechnology advances
pub_type： 杂志文章
doi：10.1016/0734-9750(85)90292-7
更新日期：1985-01-01 00:00:00
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journal_title：Biotechnology advances
pub_type： 杂志文章,评审
doi：10.1016/j.biotechadv.2011.03.006
更新日期：2011-11-01 00:00:00
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journal_title：Biotechnology advances
pub_type： 杂志文章
doi：10.1016/0734-9750(94)90023-x
更新日期：1994-01-01 00:00:00
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journal_title：Biotechnology advances
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journal_title：Biotechnology advances
pub_type： 杂志文章,评审
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更新日期：2012-05-01 00:00:00
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journal_title：Biotechnology advances
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journal_title：Biotechnology advances
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更新日期：2020-01-01 00:00:00
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journal_title：Biotechnology advances
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journal_title：Biotechnology advances
pub_type： 杂志文章,评审
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更新日期：2004-11-01 00:00:00
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journal_title：Biotechnology advances
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journal_title：Biotechnology advances
pub_type： 杂志文章,评审
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更新日期：2010-07-01 00:00:00
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journal_title：Biotechnology advances
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更新日期：2019-12-01 00:00:00
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journal_title：Biotechnology advances
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更新日期：2017-07-01 00:00:00
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journal_title：Biotechnology advances
pub_type： 杂志文章
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更新日期：1999-09-01 00:00:00
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journal_title：Biotechnology advances
pub_type： 杂志文章,评审
doi：10.1016/j.biotechadv.2008.04.004
更新日期：2008-11-01 00:00:00
