Abstract：
:Enzymes are essential and ubiquitous biocatalysts involved in various metabolic pathways and used in many industrial processes. Here, we reframe enzymes not just as biocatalysts transforming bioproducts but also as sensitive probes for exploring the structure and composition of complex bioproducts, like meat tissue, dairy products and plant materials, in both food and non-food bioprocesses. This review details the global strategy and presents the most recent investigations to prepare and use enzymes as relevant probes, with a focus on glycoside-hydrolases involved in plant deconstruction and proteases and lipases involved in food digestion. First, to expand the enzyme repertoire to fit bioproduct complexity, novel enzymes are mined from biodiversity and can be artificially engineered. Enzymes are further characterized by exploring sequence/structure/dynamics/function relationships together with the environmental factors influencing enzyme interactions with their substrates. Then, the most advanced experimental and theoretical approaches developed for exploring bioproducts at various scales (from nanometer to millimeter) using active and inactive enzymes as probes are illustrated. Overall, combining multimodal and multiscale approaches brings a better understanding of native-form or transformed bioproduct architecture and composition, and paves the way to mainstream the use of enzymes as probes.
journal_name
Biotechnol Advjournal_title
Biotechnology advancesauthors
Bourlieu C,Astruc T,Barbe S,Berrin JG,Bonnin E,Boutrou R,Hugouvieux V,Le Feunteun S,Paës Gdoi
10.1016/j.biotechadv.2020.107546subject
Has Abstractpub_date
2020-01-01 00:00:00pages
107546eissn
0734-9750issn
1873-1899pii
S0734-9750(20)30043-4journal_volume
41pub_type
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