Approach to maceration mechanism in enzymatic pulping of bast fibers by alkalophilic pectinolytic enzymes produced by Erwinia species.

Abstract:

:Tissue maceration was generally elucidated by the action of endo-polygalacturonase and endo-pectate or -pectin lyase (endo-PAL or -PNL). In a process of screening of Erwinia and Pseudomonas strains for enzymatic pulping of pectocellulosic bast fibers, it was found that their PAL productivity was not completely related with defibration activity, i.e., the fact that an E.chrysanthemi strain showed high PAL productivity but possessed rather low defibration activity. Moreover, defibration activity was parallel to the amount of neutral sugars released during pulping. Based on these fact, the maceration or enzymatic pulping of basts was estimated to proceed not only by cleavage of interfiber bonding cause by PAL action but also another factors. Among three possibilities proposed on the maceration mechanism of basts, it was elucidated by a concerted action of PAL and PNL with an aid of xylanase. In addition, a quantitative determinative method of maceration activity toward basts was also presented.

journal_name

Biotechnol Adv

journal_title

Biotechnology advances

authors

Kobayashi Y,Komae K,Tanabe H,Matsuo R

doi

10.1016/0734-9750(88)90572-1

subject

Has Abstract

pub_date

1988-01-01 00:00:00

pages

29-37

issue

1

eissn

0734-9750

issn

1873-1899

pii

0734975088905721

journal_volume

6

pub_type

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