Abstract:
:The potential for gene therapy to cure a wide range of diseases has lead to high expectations and a great increase in research efforts in this area. At present, viral vectors are the most efficient means of delivering a corrective gene into human cells. While a number of different viral vectors are under development, retroviral vectors are currently the most common type used in clinical trials today. However, the production of retroviral vectors for gene therapy applications faces a number of challenges. Of primary concern is the low titre of vector stocks produced by packaging cells in culture and the inherent instability of retroviral vector activity. The problems facing large-scale retroviral vector production are outlined in this review and the research efforts by a number of groups who have attempted to optimise production methods are presented.
journal_name
Biotechnol Advjournal_title
Biotechnology advancesauthors
McTaggart S,Al-Rubeai Mdoi
10.1016/s0734-9750(01)00087-8subject
Has Abstractpub_date
2002-04-01 00:00:00pages
1-31issue
1eissn
0734-9750issn
1873-1899pii
S0734975001000878journal_volume
20pub_type
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journal_title:Biotechnology advances
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pub_type: 杂志文章
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journal_title:Biotechnology advances
pub_type: 杂志文章,评审
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journal_title:Biotechnology advances
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journal_title:Biotechnology advances
pub_type: 杂志文章,评审
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journal_title:Biotechnology advances
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pub_type: 杂志文章
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journal_title:Biotechnology advances
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