Abstract：
:Long-chain polyunsaturated fatty acids (LC-PUFAs) especially ω-3 fatty acids provide significant health benefits for human beings. However, ω-3 LC-PUFAs cannot be synthesized de novo in mammals. Traditionally, ω-3 LC-PUFAs are extracted from marine fish, and their production depends on sea fishing, which has not met ever-increasing global demand. To address the challenges, innovative cellular engineering strategies need to be developed. In nature, many fungi and microalgae are rich in ω-3 LC-PUFAs, representing promising sources of ω-3 LC-PUFAs. The latest progress in developing new cellular engineering strategies toward sustainable ω-3 LC-PUFAs production using fungi and microalga has demonstrated that they can to some extent address the supply shortage. In this review, we critically summarize the recent progress in enhancing the productivity in various ω-3 LC-PUFAs-producing organisms, as well as the latest efforts of biosynthesizing PUFAs in heterogenous biosystems. In addition, we also provide future perspectives in developing genetic toolkits for LC-PUFAs producing microbes so that cut-edging biotechnology such as gene stacking and genome editing can be further applied to increase the productivity of ω-3 LC-PUFAs.
journal_name
Biotechnol Advjournal_title
Biotechnology advancesauthors
Diao J,Song X,Guo T,Wang F,Chen L,Zhang Wdoi
10.1016/j.biotechadv.2019.107497subject
Has Abstractpub_date
2020-01-01 00:00:00pages
107497eissn
0734-9750issn
1873-1899pii
S0734-9750(19)30197-1journal_volume
40pub_type
杂志文章,评审abstract：:Yersinia pestis, the causative agent of bubonic and pneumonic plague, is an extremely virulent bacterium but there are currently no approved vaccines for protection against this organism. Plants represent an economical and safer alternative to fermentation-based expression systems for the production of therapeutic pro...
journal_title：Biotechnology advances
pub_type： 杂志文章,评审
doi：10.1016/j.biotechadv.2009.11.006
更新日期：2010-01-01 00:00:00
abstract：:It has recently been discovered that a number of synthetic chemicals and naturally occurring compounds released into the environment can influence endocrine activity. These endocrine-disrupting compounds (EDCs) are highly varied in structure, provenance, and mode of action (MOA). Many EDCs are anthropogenic, products ...
journal_title：Biotechnology advances
pub_type： 杂志文章,评审
doi：10.1016/j.biotechadv.2003.08.005
更新日期：2003-12-01 00:00:00
abstract：:The byproduct of cheese-producing industries, cheese whey, is considered as an environmental pollutant due to its high BOD and COD concentrations. The high organic load of whey arises from the presence of residual milk nutrients. As demand for milk-derived products is increasing, it leads to increased production of wh...
journal_title：Biotechnology advances
pub_type： 杂志文章,评审
doi：10.1016/j.biotechadv.2015.07.002
更新日期：2015-11-01 00:00:00
abstract：:Cryptosporidium parvum is an obligate protozoan parasite responsible for the diarrheal illness cryptosporidiosis in humans and animals. Although C. parvum is particularly pathogenic in immunocompromised hosts, the molecular mechanisms by which C. parvum invades the host epithelial cells are not well understood. Charac...
journal_title：Biotechnology advances
pub_type： 杂志文章,评审
doi：10.1016/j.biotechadv.2006.08.003
更新日期：2007-01-01 00:00:00
abstract：:The high plasticity of the active-site cavity of cytochromes P450, permitting reactivity toward a vast array of compounds, makes these enzymes attractive targets for biotechnological application. Escalating attention in this area is driven by remarkable progress in the rational design by DNA shuffling of self-sufficie...
journal_title：Biotechnology advances
pub_type： 杂志文章,评审
doi：10.1016/j.biotechadv.2008.10.001
更新日期：2009-03-01 00:00:00
abstract：:This review discusses peptide-based vaccines in breast cancer, immune responses and clinical outcomes, which include studies on animal models and phase I, phase I/II, phase II and phase III clinical trials. Peptide-based vaccines are powerful neoadjuvant immunotherapies that can directly target proteins expressed in t...
journal_title：Biotechnology advances
pub_type： 杂志文章,评审
doi：10.1016/j.biotechadv.2015.10.013
更新日期：2015-12-01 00:00:00
abstract：:Fungi are notoriously prolific producers of secondary metabolites including nonribosomal peptides (NRPs). The structural complexity of NRPs grants them interesting activities such as antibiotic, anti-cancer, and anti-inflammatory properties. The discovery of these compounds with attractive activities can be achieved b...
journal_title：Biotechnology advances
pub_type： 杂志文章,评审
doi：10.1016/j.biotechadv.2019.107449
更新日期：2019-12-01 00:00:00
abstract：:The phenomenon of metal biosorption by microorganisms has been thoroughly documented. Although this phenomenon is exhibited by both living and non-living forms of biomass, the purpose of this chapter will be to review biosorption by the latter. In addition, the application of various technological processes required f...
journal_title：Biotechnology advances
pub_type： 杂志文章
doi：10.1016/0734-9750(94)90005-1
更新日期：1994-01-01 00:00:00
abstract：:2-Phenylethanol (2-PE) is an important aromatic alcohol with a rose-like fragrance. It has been widely applied in the cosmetic, perfume, and food industries and is mainly produced by chemical synthesis. An alternative method for the production of natural flavors and fragrances is the microbial transformation process, ...
journal_title：Biotechnology advances
pub_type： 杂志文章,评审
doi：10.1016/j.biotechadv.2011.05.001
更新日期：2011-11-01 00:00:00
abstract：:The use of somatic embryos as artificial seeds for large scale clonal propagation of plants is close to becoming a reality. The quality of the artificial seed depends on the temporal, quantitative and qualitative supply of growth regulator and nutrients along with an optimal physical environment. Desiccation of somati...
journal_title：Biotechnology advances
pub_type： 杂志文章
doi：10.1016/0734-9750(92)90301-o
更新日期：1992-01-01 00:00:00
abstract：:Although rational method and global technique have been successfully applied in strain improvement respectively, the demand for engineering complex phenotypes required combinatorial approach. The technology of genome shuffling has been presented as a novel whole genome engineering approach for the rapid improvement of...
journal_title：Biotechnology advances
pub_type： 杂志文章,评审
doi：10.1016/j.biotechadv.2009.05.016
更新日期：2009-11-01 00:00:00
abstract：:The non-conventional oleaginous yeast Yarrowia lipolytica shows great industrial promise. It naturally produces certain compounds of interest but can also artificially generate non-native metabolites, thanks to an engineering process made possible by the significant expansion of a dedicated genetic toolbox. In this re...
journal_title：Biotechnology advances
pub_type： 杂志文章,评审
doi：10.1016/j.biotechadv.2018.10.004
更新日期：2018-12-01 00:00:00
abstract：:In the biological milieu of a cell, soluble crowding molecules and rigid confined environments strongly influence whether the protein is properly folded, intrinsically disordered proteins assemble into distinct phases, or a denatured or aggregated protein species is favored. Such crowding and confinement factors act t...
journal_title：Biotechnology advances
pub_type： 杂志文章,评审
doi：10.1016/j.biotechadv.2020.107573
更新日期：2020-01-01 00:00:00
abstract：:The present review provides up-to-date information on the occurrence and methodologies used for producing and purifying endo-β-mannanases and a comprehensive comparison of their biochemical properties. The amalgamation of biochemical, molecular and structural biology approaches which have been used for understanding e...
journal_title：Biotechnology advances
pub_type： 杂志文章,评审
doi：10.1016/j.biotechadv.2016.11.001
更新日期：2017-01-01 00:00:00
abstract：:During a tempe fermentation the concentrations of linoleic, and alpha-linolenic acids (ALA) decreased while the concentration of oleic acid increased. During fatty acid synthesis Rhizopus sp. produced only gamma-linolenic acid (GLA) instead of ALA. The amount of GLA in tempe were influenced by varying external paramet...
journal_title：Biotechnology advances
pub_type： 杂志文章
doi：10.1016/0734-9750(93)90016-g
更新日期：1993-01-01 00:00:00
abstract：:Dihydroxyacetone is extensively used in cosmetic industry as an artificial suntan besides having clinical and biological applications. Thus, it is important to meet the commercial demand of dihydroxyacetone at an economical and qualitative level. Microbial route of production is found to be more favorable for dihydrox...
journal_title：Biotechnology advances
pub_type： 杂志文章,收录出版,评审
doi：10.1016/j.biotechadv.2008.02.001
更新日期：2008-07-01 00:00:00
abstract：:Worldwide, colorectal cancer (CRC) remains a major cancer type and leading cause of death. Unfortunately, current medical treatments are not sufficient due to lack of effective therapy, adverse side effects, chemoresistance and disease recurrence. In recent decades, epidemiologic observations have highlighted the asso...
journal_title：Biotechnology advances
pub_type： 杂志文章,评审
doi：10.1016/j.biotechadv.2018.11.011
更新日期：2020-01-01 00:00:00
abstract：:The risk of acquisition of resistance to chemotherapy remains a major hurdle in the management of various types of cancer patients. Several cellular and noncellular mechanisms are involved in developing both intrinsic and acquired resistance in cancer cells toward chemotherapy. This review covers the various multidrug...
journal_title：Biotechnology advances
pub_type： 杂志文章,评审
doi：10.1016/j.biotechadv.2013.06.004
更新日期：2013-12-01 00:00:00
abstract：:Product inhibition of cellulolytic enzymes affects the efficiency of the biocatalytic conversion of lignocellulosic biomass to ethanol and other valuable products. New strategies that focus on reactor designs encompassing product removal, notably glucose removal, during enzymatic cellulose conversion are required for ...
journal_title：Biotechnology advances
pub_type： 杂志文章
doi：10.1016/j.biotechadv.2010.02.005
更新日期：2010-05-01 00:00:00
abstract：:Silver was widely used in medicine to treat bacterial infections in the 19th and early 20th century, up until the discovery and development of the first modern antibiotics in the 1940s, which were markedly more effective. Since then, every new antibiotic introduced to the clinic has led to an associated development of...
journal_title：Biotechnology advances
pub_type： 杂志文章,评审
doi：10.1016/j.biotechadv.2018.05.004
更新日期：2018-01-01 00:00:00
abstract：:Bradyrhizobium elkanii produces rhizobitoxine, an enol-ether amino acid, which has been regarded as a phytotoxin because it causes chlorosis in soybeans. However, recent studies have revealed that rhizobitoxine plays a positive role in establishing symbiosis between B. elkanii and host legumes: rhizobitoxine enhances ...
journal_title：Biotechnology advances
pub_type： 杂志文章,评审
doi：10.1016/j.biotechadv.2006.01.004
更新日期：2006-07-01 00:00:00
abstract：:Human immunodeficiency virus (HIV) remains a worldwide health problem despite huge investments and research breakthroughs, and no single drug is effective in killing the virus yet. Among new strategies to control HIV infection, the phage display (PD) technology has become a promising tool in the discovery of peptides ...
journal_title：Biotechnology advances
pub_type： 杂志文章,评审
doi：10.1016/j.biotechadv.2018.07.003
更新日期：2018-11-15 00:00:00
abstract：:DNA vaccination has been widely explored to develop new, alternative and efficient vaccines for cancer immunotherapy. DNA vaccines offer several benefits such as specific targeting, use of multiple genes to enhance immunity and reduced risk compared to conventional vaccines. Rapid developments in molecular biology and...
journal_title：Biotechnology advances
pub_type： 杂志文章,评审
doi：10.1016/j.biotechadv.2011.06.020
更新日期：2012-01-01 00:00:00
abstract：:Complete profiling would substantially facilitate the fundamental understanding of tumor angiogenesis and of possible anti-angiogenesis cancer treatments. We developed an integrated synchrotron-based methodology with excellent performances: detection of very small vessels by high spatial resolution (~1 μm) and nanopar...
journal_title：Biotechnology advances
pub_type： 杂志文章
doi：10.1016/j.biotechadv.2011.12.001
更新日期：2013-05-01 00:00:00
abstract：:One of the major bottlenecks in automation and process control of industrial bioprocesses is the lack of suitable sensing devices to accurately measure the concentrations of biomolecules. The measurement of ions (e.g., H(+), NH(4)(+)) and gases (e.g., O(2), CO(2), NH(3)) using standard ion-selective and gas sensing el...
journal_title：Biotechnology advances
pub_type： 杂志文章
doi：10.1016/0734-9750(85)90292-7
更新日期：1985-01-01 00:00:00
abstract：:Direct interspecies electron transfer (DIET) via electrically conductive pili (e-pili) and c-type cytochrome between acetogens and methanogens has been proposed as an essential pathway for methane production. Supplements of conductive materials have been extensively found to promote methane production in microbial ana...
journal_title：Biotechnology advances
pub_type： 杂志文章,评审
doi：10.1016/j.biotechadv.2019.107443
更新日期：2019-12-01 00:00:00
abstract：:We review the recent development of mobile detection instruments used for medical diagnostics, and consider the relative advantages of approaches that utilize the internal sensing capabilities of commercially available mobile communication devices (such as smartphones and tablet computers) compared to those that utili...
journal_title：Biotechnology advances
pub_type： 杂志文章,评审
doi：10.1016/j.biotechadv.2016.02.010
更新日期：2016-05-01 00:00:00
abstract：:Yeast strains Y1, Y4 and Y7 demonstrated high conversion efficiencies for sugars and high abilities to tolerate or metabolize inhibitors in dilute-acid lignocellulosic hydrolysates. Strains Y1 and Y4 completely consumed the glucose within 24 h in dilute-acid lignocellulosic hydrolysate during in situ detoxification, a...
journal_title：Biotechnology advances
pub_type： 杂志文章
doi：10.1016/j.biotechadv.2009.04.008
更新日期：2009-09-01 00:00:00
abstract：:For many proteins and peptides, disulfide bridges are prerequisite for their proper biological function. Many commercialized proteins are crosslinked by disulfide bridges that increase their resistance to destructive effects of extreme environment used in industrial processes or protect protein-based therapeutics from...
journal_title：Biotechnology advances
pub_type： 杂志文章,评审
doi：10.1016/j.biotechadv.2004.09.002
更新日期：2005-01-01 00:00:00
abstract：:In recent years, the use of surface-display vectors for displaying polypeptides on the surface of bacteriophage and bacteria, combined with in vitro selection technologies, has transformed the way in which we generate and manipulate ligands, such as enzymes, antibodies and peptides. Phage display is based on expressin...
journal_title：Biotechnology advances
pub_type： 杂志文章
doi：10.1016/s0734-9750(00)00054-9
更新日期：2001-02-01 00:00:00
