Synthetic biology tools for engineering Yarrowia lipolytica.

Abstract:

:The non-conventional oleaginous yeast Yarrowia lipolytica shows great industrial promise. It naturally produces certain compounds of interest but can also artificially generate non-native metabolites, thanks to an engineering process made possible by the significant expansion of a dedicated genetic toolbox. In this review, we present recently developed synthetic biology tools that facilitate the manipulation of Y. lipolytica, including 1) DNA assembly techniques, 2) DNA parts for constructing expression cassettes, 3) genome-editing techniques, and 4) computational tools.

journal_name

Biotechnol Adv

journal_title

Biotechnology advances

authors

Larroude M,Rossignol T,Nicaud JM,Ledesma-Amaro R

doi

10.1016/j.biotechadv.2018.10.004

subject

Has Abstract

pub_date

2018-12-01 00:00:00

pages

2150-2164

issue

8

eissn

0734-9750

issn

1873-1899

pii

S0734-9750(18)30167-8

journal_volume

36

pub_type

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