Abstract：
:Quantitative real-time PCR (qPCR) has been widely used in recent environmental microbial ecology studies as a tool for detecting and quantifying microorganisms of interest, which aids in better understandings of the complexity of wastewater microbial communities. Although qPCR can be used to provide more specific and accurate quantification than other molecular techniques, it does have limitations that must be considered when applying it in practice. This article reviews the principle of qPCR quantification and its applications to microbial ecology studies in various wastewater treatment environments. Here we also address several limitations of qPCR-based approaches that can affect the validity of quantification data: template nucleic acid quality, nucleic acid extraction efficiency, specificity of group-specific primers and probes, amplification of nonviable DNA, gene copy number variation, and limited number of sequences in the database. Even with such limitations, qPCR is reportedly among the best methods for quantitatively investigating environmental microbial communities. The application of qPCR is and will continue to be increasingly common in studies of wastewater treatment systems. To obtain reliable analyses, however, the limitations that have often been overlooked must be carefully considered when interpreting the results.
journal_name
Biotechnol Advjournal_title
Biotechnology advancesauthors
Kim J,Lim J,Lee Cdoi
10.1016/j.biotechadv.2013.05.010subject
Has Abstractpub_date
2013-12-01 00:00:00pages
1358-73issue
8eissn
0734-9750issn
1873-1899pii
S0734-9750(13)00091-8journal_volume
31pub_type
杂志文章,评审abstract：:Plant cell suspension culture (PCSC) has emerged as a viable technology to produce plant specialized metabolites (PSM). While Taxol® and ginsenoside are two examples of successfully commercialized PCSC-derived PSM, widespread utilization of the PCSC platform has yet to be realized primarily due to a lack of understand...
journal_title：Biotechnology advances
pub_type： 杂志文章,评审
doi：10.1016/j.biotechadv.2020.107635
更新日期：2020-12-01 00:00:00
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journal_title：Biotechnology advances
pub_type： 杂志文章,评审
doi：10.1016/j.biotechadv.2010.11.007
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journal_title：Biotechnology advances
pub_type： 杂志文章,评审
doi：10.1016/j.biotechadv.2020.107581
更新日期：2020-01-01 00:00:00
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journal_title：Biotechnology advances
pub_type： 杂志文章
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更新日期：1992-01-01 00:00:00
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journal_title：Biotechnology advances
pub_type： 杂志文章,评审
doi：10.1016/j.biotechadv.2016.11.001
更新日期：2017-01-01 00:00:00
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journal_title：Biotechnology advances
pub_type： 杂志文章,评审
doi：10.1016/j.biotechadv.2012.02.002
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journal_title：Biotechnology advances
pub_type： 杂志文章,评审
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journal_title：Biotechnology advances
pub_type： 杂志文章
doi：10.1016/0734-9750(84)90010-7
更新日期：1984-01-01 00:00:00
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journal_title：Biotechnology advances
pub_type： 杂志文章,评审
doi：10.1016/j.biotechadv.2011.01.003
更新日期：2011-05-01 00:00:00
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journal_title：Biotechnology advances
pub_type： 杂志文章,评审
doi：10.1016/j.biotechadv.2011.06.020
更新日期：2012-01-01 00:00:00
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journal_title：Biotechnology advances
pub_type： 杂志文章,评审
doi：10.1016/j.biotechadv.2018.03.020
更新日期：2018-01-01 00:00:00
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journal_title：Biotechnology advances
pub_type： 杂志文章,评审
doi：10.1016/j.biotechadv.2015.11.003
更新日期：2016-03-01 00:00:00
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journal_title：Biotechnology advances
pub_type： 杂志文章
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更新日期：1983-01-01 00:00:00
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journal_title：Biotechnology advances
pub_type： 杂志文章,评审
doi：10.1016/j.biotechadv.2008.04.004
更新日期：2008-11-01 00:00:00
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journal_title：Biotechnology advances
pub_type： 杂志文章,评审
doi：10.1016/j.biotechadv.2015.05.008
更新日期：2015-11-01 00:00:00
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journal_title：Biotechnology advances
pub_type： 杂志文章,评审
doi：10.1016/j.biotechadv.2013.08.019
更新日期：2014-01-01 00:00:00
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journal_title：Biotechnology advances
pub_type： 杂志文章
doi：10.1016/0734-9750(93)90411-f
更新日期：1993-01-01 00:00:00
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journal_title：Biotechnology advances
pub_type： 杂志文章,评审
doi：10.1016/j.biotechadv.2006.08.003
更新日期：2007-01-01 00:00:00
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journal_title：Biotechnology advances
pub_type： 杂志文章,评审
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更新日期：2015-12-01 00:00:00
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journal_title：Biotechnology advances
pub_type： 杂志文章
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更新日期：1991-01-01 00:00:00
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journal_title：Biotechnology advances
pub_type： 杂志文章,评审
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更新日期：2018-01-01 00:00:00
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journal_title：Biotechnology advances
pub_type： 杂志文章,评审
doi：10.1016/j.biotechadv.2009.01.003
更新日期：2009-05-01 00:00:00
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journal_title：Biotechnology advances
pub_type： 杂志文章,评审
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更新日期：2014-05-01 00:00:00
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journal_title：Biotechnology advances
pub_type： 杂志文章,评审
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更新日期：2020-01-01 00:00:00
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journal_title：Biotechnology advances
pub_type： 杂志文章,评审
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更新日期：2020-01-01 00:00:00
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journal_title：Biotechnology advances
pub_type： 杂志文章,评审
doi：10.1016/j.biotechadv.2009.05.016
更新日期：2009-11-01 00:00:00
abstract：:A rapidly growing body of experimental evidence has begun to shed light on the wide ranging molecular mechanisms which modulate intra- and inter-cellular communications. A substantial quantity of the available knowledge has only been uncovered in recent years, and we are learning that donor cells release nanovesicles,...
journal_title：Biotechnology advances
pub_type： 杂志文章,评审
doi：10.1016/j.biotechadv.2017.12.010
更新日期：2018-01-01 00:00:00
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journal_title：Biotechnology advances
pub_type： 杂志文章
doi：10.1016/j.biotechadv.2011.12.001
更新日期：2013-05-01 00:00:00
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journal_title：Biotechnology advances
pub_type： 杂志文章,评审
doi：10.1016/j.biotechadv.2012.11.004
更新日期：2013-09-01 00:00:00
abstract：:Small molecule metabolites secreted by pathological processes can act as molecular biomarkers for clinical diagnosis. In vitro detection of the metabolites such as glucose and reactive oxygen species is of great significance for precise screening, monitoring and prognosis of metabolic disorders and relevant diseases s...
journal_title：Biotechnology advances
pub_type： 杂志文章,评审
doi：10.1016/j.biotechadv.2016.01.006
更新日期：2016-05-01 00:00:00
