Abstract：
:Osteochondral defect management and repair remain a significant challenge in orthopedic surgery. Osteochondral defects contain damage to both the articular cartilage as well as the underlying subchondral bone. In order to repair an osteochondral defect the needs of the bone, cartilage and the bone-cartilage interface must be taken into account. Current clinical treatments for the repair of osteochondral defects have only been palliative, not curative. Tissue engineering has emerged as a potential alternative as it can be effectively used to regenerate bone, cartilage and the bone-cartilage interface. Several scaffold strategies, such as single phase, layered, and recently graded structures have been developed and evaluated for osteochondral defect repair. Also, as a potential cell source, tissue specific cells and progenitor cells are widely studied in cell culture models, as well with the osteochondral scaffolds in vitro and in vivo. Novel factor strategies being developed, including single factor, multi-factor, or controlled factor release in a graded fashion, not only assist bone and cartilage regeneration, but also establish osteochondral interface formation. The field of tissue engineering has made great strides, however further research needs to be carried out to make this strategy a clinical reality. In this review, we summarize current tissue engineering strategies, including scaffold design, bioreactor use, as well as cell and factor based approaches and recent developments for osteochondral defect repair. In addition, we discuss various challenges that need to be addressed in years to come.
journal_name
Biotechnol Advjournal_title
Biotechnology advancesauthors
Nukavarapu SP,Dorcemus DLdoi
10.1016/j.biotechadv.2012.11.004subject
Has Abstractpub_date
2013-09-01 00:00:00pages
706-21issue
5eissn
0734-9750issn
1873-1899pii
S0734-9750(12)00189-9journal_volume
31pub_type
杂志文章,评审abstract：:Due to concerns about the unsustainability and predictable shortage of fossil feedstocks, research efforts are currently being made to develop new processes for production of commodities using alternative feedstocks. 3-Hydroxypropionic acid (CAS 503-66-2) was recognised by the US Department of Energy as one of the mos...
journal_title：Biotechnology advances
pub_type： 杂志文章,评审
doi：10.1016/j.biotechadv.2018.03.020
更新日期：2018-01-01 00:00:00
abstract：:This review elaborates on the recent development of microbial sterol biotransformation systems. Particular emphasis is laid on the new enzymatic approach investigating the cleavage of sterol side chain. New developments in the area of immobilized cell system and use of organic media along with recent reviews on side c...
journal_title：Biotechnology advances
pub_type： 杂志文章
doi：10.1016/0734-9750(92)91351-e
更新日期：1992-01-01 00:00:00
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journal_title：Biotechnology advances
pub_type： 杂志文章,评审
doi：10.1016/j.biotechadv.2008.07.003
更新日期：2009-01-01 00:00:00
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journal_title：Biotechnology advances
pub_type： 杂志文章,评审
doi：10.1016/j.biotechadv.2014.02.002
更新日期：2014-05-01 00:00:00
abstract：:Lactic acid, the most important hydroxycarboxylic acid, is now commercially produced by the fermentation of sugars present in biomass. In addition to its use in the synthesis of biodegradable polymers, lactic acid can be regarded as a feedstock for the green chemistry of the future. Different potentially useful chemic...
journal_title：Biotechnology advances
pub_type： 杂志文章,评审
doi：10.1016/j.biotechadv.2011.07.022
更新日期：2011-11-01 00:00:00
abstract：:In addition to the nuclear genome, organisms have organelle genomes. Most of the DNA present in eukaryotic organisms is located in the cell nucleus. Chloroplasts have independent genomes which are inherited from the mother. Duplicated genes are common in the genomes of all organisms. It is believed that gene duplicati...
journal_title：Biotechnology advances
pub_type： 杂志文章,评审
doi：10.1016/j.biotechadv.2009.01.012
更新日期：2009-07-01 00:00:00
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journal_title：Biotechnology advances
pub_type： 杂志文章,评审
doi：10.1016/j.biotechadv.2013.11.010
更新日期：2014-01-01 00:00:00
abstract：:Cryptosporidium parvum is an obligate protozoan parasite responsible for the diarrheal illness cryptosporidiosis in humans and animals. Although C. parvum is particularly pathogenic in immunocompromised hosts, the molecular mechanisms by which C. parvum invades the host epithelial cells are not well understood. Charac...
journal_title：Biotechnology advances
pub_type： 杂志文章,评审
doi：10.1016/j.biotechadv.2006.08.003
更新日期：2007-01-01 00:00:00
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journal_title：Biotechnology advances
pub_type： 杂志文章,评审
doi：10.1016/j.biotechadv.2003.08.005
更新日期：2003-12-01 00:00:00
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journal_title：Biotechnology advances
pub_type： 杂志文章
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更新日期：1994-01-01 00:00:00
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journal_title：Biotechnology advances
pub_type： 杂志文章,评审
doi：10.1016/j.biotechadv.2017.11.004
更新日期：2018-01-01 00:00:00
abstract：:Biomass, a renewable, sustainable and carbon dioxide neutral resource, has received widespread attention in the energy market as an alternative to fossil fuels. Thermal-chemical conversion of biomass to produce biofuels is a promising technology with many commercial applications. This paper reviewed the state-of-the-a...
journal_title：Biotechnology advances
pub_type： 杂志文章
doi：10.1016/j.biotechadv.2012.01.016
更新日期：2012-07-01 00:00:00
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journal_title：Biotechnology advances
pub_type： 杂志文章,评审
doi：10.1016/j.biotechadv.2018.04.009
更新日期：2018-11-01 00:00:00
abstract：:Enzymes are essential and ubiquitous biocatalysts involved in various metabolic pathways and used in many industrial processes. Here, we reframe enzymes not just as biocatalysts transforming bioproducts but also as sensitive probes for exploring the structure and composition of complex bioproducts, like meat tissue, d...
journal_title：Biotechnology advances
pub_type： 杂志文章,评审
doi：10.1016/j.biotechadv.2020.107546
更新日期：2020-01-01 00:00:00
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journal_title：Biotechnology advances
pub_type： 杂志文章,评审
doi：10.1016/j.biotechadv.2011.04.001
更新日期：2011-11-01 00:00:00
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journal_title：Biotechnology advances
pub_type： 杂志文章
doi：10.1016/0734-9750(94)90291-7
更新日期：1994-01-01 00:00:00
abstract：:Orchids (Orchidaceae) are one of the most diverse plant groups on the planet with over 25,000 species. For over a century, scientists and horticulturalists have been fascinated by their complex floral morphology, pollinator specificity and multiple ethnobotanical uses, including as food, flavourings, medicines, orname...
journal_title：Biotechnology advances
pub_type： 杂志文章,评审
doi：10.1016/j.biotechadv.2016.01.001
更新日期：2016-07-01 00:00:00
abstract：:Vaccines represent a special class of drugs, capable of stimulating immune system responses against pathogens and tumors. Vaccine development is a lengthy process that includes expensive laboratory experiments in order to assess safety and effectiveness. As the efficacy of a vaccine was demonstrated by biological/chem...
journal_title：Biotechnology advances
pub_type： 杂志文章,评审
doi：10.1016/j.biotechadv.2009.10.001
更新日期：2010-01-01 00:00:00
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journal_title：Biotechnology advances
pub_type： 杂志文章
doi：10.1016/0734-9750(93)90411-f
更新日期：1993-01-01 00:00:00
abstract：:Biotechnology techniques involving plant tissue culture and recombinant DNA technologies are powerful tools that can complement conventional breeding and expedite Capsicum improvement. The rate of progress in Capsicum is relatively slower than other members of Solanaceae because of its high genotypic dependence and re...
journal_title：Biotechnology advances
pub_type： 杂志文章,评审
doi：10.1016/j.biotechadv.2009.08.005
更新日期：2010-01-01 00:00:00
abstract：:Product inhibition of cellulolytic enzymes affects the efficiency of the biocatalytic conversion of lignocellulosic biomass to ethanol and other valuable products. New strategies that focus on reactor designs encompassing product removal, notably glucose removal, during enzymatic cellulose conversion are required for ...
journal_title：Biotechnology advances
pub_type： 杂志文章
doi：10.1016/j.biotechadv.2010.02.005
更新日期：2010-05-01 00:00:00
abstract：:Long-chain polyunsaturated fatty acids (LC-PUFAs) especially ω-3 fatty acids provide significant health benefits for human beings. However, ω-3 LC-PUFAs cannot be synthesized de novo in mammals. Traditionally, ω-3 LC-PUFAs are extracted from marine fish, and their production depends on sea fishing, which has not met e...
journal_title：Biotechnology advances
pub_type： 杂志文章,评审
doi：10.1016/j.biotechadv.2019.107497
更新日期：2020-01-01 00:00:00
abstract：:The yeast, Saccharomyces cerevisiae is currently used for the production of recombinant DNA-generated proteins derived from a variety of eukaryotic organisms. The applications of a yeast-based technology in the production of proteins for pharmaceutical and industrial purposes is discussed including current methods for...
journal_title：Biotechnology advances
pub_type： 杂志文章
doi：10.1016/0734-9750(89)90357-1
更新日期：1989-01-01 00:00:00
abstract：:The marine ecosystem is still an untapped reservoir of biologically active compounds, which have considerable potential to supply food ingredients towards development of new functional foods. With the goal of increasing the availability and chemical diversity of functional marine ingredients, much research has been de...
journal_title：Biotechnology advances
pub_type： 杂志文章,评审
doi：10.1016/j.biotechadv.2012.03.006
更新日期：2012-11-01 00:00:00
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journal_title：Biotechnology advances
pub_type： 杂志文章,评审
doi：10.1016/j.biotechadv.2013.06.004
更新日期：2013-12-01 00:00:00
abstract：:Malolactic fermentation (MLF) in wine can be accomplished by relying on the natural microflora or by inducing through inoculation of a specific strain(s) of malolactic bacteria, primarily strains of Leuconostoc oenos. Problems with inducing MLF include intrinsic factors of the grape must such as pH, presence of sulfur...
journal_title：Biotechnology advances
pub_type： 杂志文章
doi：10.1016/0734-9750(89)90179-1
更新日期：1989-01-01 00:00:00
abstract：:Within five years, the CRISPR-Cas system has emerged as the dominating tool for genome engineering, while also changing the speed and efficiency of metabolic engineering in conventional (Saccharomyces cerevisiae and Schizosaccharomyces pombe) and non-conventional (Yarrowia lipolytica, Pichia pastoris syn. Komagataella...
journal_title：Biotechnology advances
pub_type： 杂志文章,评审
doi：10.1016/j.biotechadv.2018.01.006
更新日期：2018-01-01 00:00:00
abstract：:Surface Enhanced Laser Desorption/Ionisation Time-of-Fight Mass Spectrometry (SELDI-TOF MS) is a technique by which protein profiles can be rapidly produced from a wide variety of biological samples. By employing chromatographic surfaces combined with the specificity and reproducibility of mass spectrometry it has all...
journal_title：Biotechnology advances
pub_type： 杂志文章,评审
doi：10.1016/j.biotechadv.2008.10.007
更新日期：2009-03-01 00:00:00
abstract：:Gene delivery vectors must deliver their cargoes into the cytosol or the nucleus, where DNA or siRNA functions in vivo. Therefore it is crucial for the rational design of the nucleic acid delivery carriers. Compared with viral vectors, non-viral vectors have overcome some fatal defections in gene therapy. Whereas the ...
journal_title：Biotechnology advances
pub_type： 杂志文章,评审
doi：10.1016/j.biotechadv.2013.11.004
更新日期：2014-07-01 00:00:00
abstract：:The degradation of plant biomass is a major challenge towards the production of bio-based compounds and materials. As key lignocellulolytic enzyme producers, filamentous fungi represent a promising reservoir to tackle this challenge. Among them, the coprophilous ascomycete Podospora anserina has been used as a model o...
journal_title：Biotechnology advances
pub_type： 杂志文章,评审
doi：10.1016/j.biotechadv.2016.05.010
更新日期：2016-09-01 00:00:00
