Abstract:
:Pichia pastoris, a methylotrophic yeast, is an established system for the production of heterologous proteins, particularly biopharmaceuticals and industrial enzymes. To maximise and optimise the production of recombinant products, recent molecular research has focused on numerous issues including the design of expression vectors, optimisation of gene copy number, co-expression of secretory proteins such as chaperones, engineering of glycosylation and secretory pathways, etc. However, the physiological effects of different cultivation strategies are often difficult to separate from the molecular effects of the gene construct (e.g., cellular stress through over-expression or incorrect post-translational processing). Hence, overall system optimisation is difficult, even though it is urgently required in order to describe and understand the behaviour of new molecular constructs. This review focuses on particular aspects of recombinant protein production related to variations in biomass growth and their implications for strain design and screening, as well as on the concept of rational comparisons between cultivation systems for the development of specific production processes in bioreactors. The relationship between specific formation rates of secreted recombinant proteins, qp, and specific growth rates, μ, has been analysed in a conceptual attempt to compare different systems, particularly those based on AOX1/methanol and GAP/glucose, and this has now evolved into a pivotal concept for bioprocess engineering of P. pastoris.
journal_name
Biotechnol Advjournal_title
Biotechnology advancesauthors
Looser V,Bruhlmann B,Bumbak F,Stenger C,Costa M,Camattari A,Fotiadis D,Kovar Kdoi
10.1016/j.biotechadv.2015.05.008subject
Has Abstractpub_date
2015-11-01 00:00:00pages
1177-93issue
6 Pt 2eissn
0734-9750issn
1873-1899pii
S0734-9750(15)30007-0journal_volume
33pub_type
杂志文章,评审abstract::Lignin is a major component of lignocellulosic biomass and as such, it is processed in enormous amounts in the pulp and paper industry worldwide. In such industry it mainly serves the purpose of a fuel to provide process steam and electricity, and to a minor extent to provide low grade heat for external purposes. Also...
journal_title:Biotechnology advances
pub_type: 杂志文章,评审
doi:10.1016/j.biotechadv.2016.10.001
更新日期:2016-12-01 00:00:00
abstract::Pigments (mainly carotenoids) are important nutraceuticals known for their potent anti-oxidant activities and have been used extensively as high end health supplements. Microalgae are the most promising sources of natural carotenoids and are devoid of the toxic effects associated with synthetic derivatives. Compared t...
journal_title:Biotechnology advances
pub_type: 杂志文章,评审
doi:10.1016/j.biotechadv.2017.09.009
更新日期:2018-01-01 00:00:00
abstract::Cyclodextrins (CDs) are a family of cyclic oligosaccharides that are composed of alpha-1,4-linked glucopyranose subunits. Cyclodextrins are produced from starch by enzymatic degradation. These macrocyclic carbohydrates with apolar internal cavities can form complexes with and solubilize many normally water-insoluble c...
journal_title:Biotechnology advances
pub_type: 杂志文章
doi:10.1016/s0734-9750(02)00020-4
更新日期:2002-12-01 00:00:00
abstract::Herbal remedies were the first medicines used by humans due to the many pharmacologically active secondary metabolites produced by plants. Some of these metabolites inhibit cell division and can therefore be used for the treatment of cancer, e.g. the mitostatic drug paclitaxel (Taxol). The ability of plants to produce...
journal_title:Biotechnology advances
pub_type: 杂志文章,评审
doi:10.1016/j.biotechadv.2018.02.002
更新日期:2018-01-01 00:00:00
abstract::The phenomenon of metal biosorption by microorganisms has been thoroughly documented. Although this phenomenon is exhibited by both living and non-living forms of biomass, the purpose of this chapter will be to review biosorption by the latter. In addition, the application of various technological processes required f...
journal_title:Biotechnology advances
pub_type: 杂志文章
doi:10.1016/0734-9750(94)90005-1
更新日期:1994-01-01 00:00:00
abstract::Human immunodeficiency virus (HIV) remains a worldwide health problem despite huge investments and research breakthroughs, and no single drug is effective in killing the virus yet. Among new strategies to control HIV infection, the phage display (PD) technology has become a promising tool in the discovery of peptides ...
journal_title:Biotechnology advances
pub_type: 杂志文章,评审
doi:10.1016/j.biotechadv.2018.07.003
更新日期:2018-11-15 00:00:00
abstract::β-Galactosidases, an important class of glycosidases, naturally catalyze the hydrolysis of β-galactosidic bonds in oligosaccharides and polysaccharides. Traditionally, these enzymes have been used to degrade lactose in dairy products, which are beneficial for lactose-intolerant people. Attractively, β-galactosidases e...
journal_title:Biotechnology advances
pub_type: 杂志文章,评审
doi:10.1016/j.biotechadv.2019.107465
更新日期:2020-01-01 00:00:00
abstract::Nucleoside derivatives are a class of compounds that have attracted intense interest in biotechnology and medicine. Use of biocatalysts opens exciting opportunities for selective synthesis of many nucleoside derivatives, and such an approach offers simplicity, exquisite selectivity and environmentally benign processes...
journal_title:Biotechnology advances
pub_type: 杂志文章,评审
doi:10.1016/j.biotechadv.2010.01.006
更新日期:2010-05-01 00:00:00
abstract::Porous aluminum oxide (PAO) is a ceramic formed by an anodization process of pure aluminum that enables the controllable assembly of exceptionally dense and regular nanopores in a planar membrane. As a consequence, PAO has a high porosity, nanopores with high aspect ratio, biocompatibility and the potential for high s...
journal_title:Biotechnology advances
pub_type: 杂志文章,评审
doi:10.1016/j.biotechadv.2011.08.005
更新日期:2012-09-01 00:00:00
abstract::Vaccines represent a special class of drugs, capable of stimulating immune system responses against pathogens and tumors. Vaccine development is a lengthy process that includes expensive laboratory experiments in order to assess safety and effectiveness. As the efficacy of a vaccine was demonstrated by biological/chem...
journal_title:Biotechnology advances
pub_type: 杂志文章,评审
doi:10.1016/j.biotechadv.2009.10.001
更新日期:2010-01-01 00:00:00
abstract::Packed bed cultivation systems have potential for widespread application in solid-state cultivation (SSC), but they are poorly characterized. The effects of particle size and substrate loading on the growth of Rhizopus oligosporus on sago-beads in packed bed bioreactors were investigated. Pressure drop and protein wer...
journal_title:Biotechnology advances
pub_type: 杂志文章
doi:10.1016/0734-9750(93)90029-m
更新日期:1993-01-01 00:00:00
abstract::Volatile organic compounds (VOCs) emitted to the environment highly probably result in ecological and health risks. Many biotechnologies for waste gases containing hydrophobic VOCs have been developed in recent years. However, these biological processes usually exhibit poor removal performances for hydrophobic VOCs du...
journal_title:Biotechnology advances
pub_type: 杂志文章,评审
doi:10.1016/j.biotechadv.2016.06.007
更新日期:2016-11-01 00:00:00
abstract::Parasitic nematodes (roundworms) of livestock have major economic impact globally. In spite of the diseases caused by these nematodes and some advances in the design of new therapeutic agents (anthelmintics) and attempts to develop vaccines against some of them, there has been limited progress in the establishment of ...
journal_title:Biotechnology advances
pub_type: 杂志文章,评审
doi:10.1016/j.biotechadv.2013.01.008
更新日期:2013-12-01 00:00:00
abstract::Phenotypic profiling of natural, engineered or synthetic cells has increasingly become a bottleneck in the mining and engineering of cell factories. Single-cell phenotyping technologies are highly promising for tackling this hurdle, yet ideally they should allow non-invasive live-cell probing, be label-free, provide l...
journal_title:Biotechnology advances
pub_type: 杂志文章,评审
doi:10.1016/j.biotechadv.2019.04.010
更新日期:2019-11-01 00:00:00
abstract::The potential for gene therapy to cure a wide range of diseases has lead to high expectations and a great increase in research efforts in this area. At present, viral vectors are the most efficient means of delivering a corrective gene into human cells. While a number of different viral vectors are under development, ...
journal_title:Biotechnology advances
pub_type: 杂志文章
doi:10.1016/s0734-9750(01)00087-8
更新日期:2002-04-01 00:00:00
abstract::Basic and applied research on microbial cellulases, hemicellulases and pectinases has not only generated significant scientific knowledge but has also revealed their enormous potential in biotechnology. At present, cellulases and related enzymes are used in food, brewery and wine, animal feed, textile and laundry, pul...
journal_title:Biotechnology advances
pub_type: 杂志文章
doi:10.1016/s0734-9750(00)00041-0
更新日期:2000-08-01 00:00:00
abstract::Polyamines (PAs) are ubiquitous biogenic amines that have been implicated in diverse cellular functions in widely distributed organisms. In plants, mutant and transgenic plants with altered activity pointed to their involvement with different abiotic and biotic stresses. Furthermore, microarray, transcriptomic and pro...
journal_title:Biotechnology advances
pub_type: 杂志文章,评审
doi:10.1016/j.biotechadv.2011.01.003
更新日期:2011-05-01 00:00:00
abstract::Selenium is an essential trace element needed for all living organisms. Despite its essentiality, selenium is a potential toxic element to natural ecosystems due to its bioaccumulation potential. Though selenium is found naturally in the earth's crust, especially in carbonate rocks and volcanic and sedimentary soils, ...
journal_title:Biotechnology advances
pub_type: 杂志文章,评审
doi:10.1016/j.biotechadv.2016.05.005
更新日期:2016-09-01 00:00:00
abstract::During a tempe fermentation the concentrations of linoleic, and alpha-linolenic acids (ALA) decreased while the concentration of oleic acid increased. During fatty acid synthesis Rhizopus sp. produced only gamma-linolenic acid (GLA) instead of ALA. The amount of GLA in tempe were influenced by varying external paramet...
journal_title:Biotechnology advances
pub_type: 杂志文章
doi:10.1016/0734-9750(93)90016-g
更新日期:1993-01-01 00:00:00
abstract::The diversity of natural compounds is essential for their mechanism of action. The source, structures and structure activity relationship of natural compounds contributed to the development of new classes of chemotherapy agents for over 40 years. The availability of combinatorial chemistry and high-throughput screenin...
journal_title:Biotechnology advances
pub_type: 杂志文章,评审
doi:10.1016/j.biotechadv.2018.04.009
更新日期:2018-11-01 00:00:00
abstract::Fungi are notoriously prolific producers of secondary metabolites including nonribosomal peptides (NRPs). The structural complexity of NRPs grants them interesting activities such as antibiotic, anti-cancer, and anti-inflammatory properties. The discovery of these compounds with attractive activities can be achieved b...
journal_title:Biotechnology advances
pub_type: 杂志文章,评审
doi:10.1016/j.biotechadv.2019.107449
更新日期:2019-12-01 00:00:00
abstract::The world population now is 6.7 billion and is predicted to reach 9 billion by 2050. Such a rapid growing population has tremendously increased the challenge for food security. Obviously, it is impossible for traditional agriculture to ensure the food security, while plant biotechnology offers considerable potential t...
journal_title:Biotechnology advances
pub_type: 杂志文章,评审
doi:10.1016/j.biotechadv.2011.10.008
更新日期:2012-05-01 00:00:00
abstract::NADPH oxidase (NOX) is a key player in the network of reactive oxygen species (ROS) producing enzymes. It catalyzes the production of superoxide (O2(-)), that in turn regulates a wide range of biological functions in a broad range of organisms. Plant Noxes are known as respiratory burst oxidase homologs (Rbohs) and ar...
journal_title:Biotechnology advances
pub_type: 杂志文章,评审
doi:10.1016/j.biotechadv.2014.02.002
更新日期:2014-05-01 00:00:00
abstract::Small molecule metabolites secreted by pathological processes can act as molecular biomarkers for clinical diagnosis. In vitro detection of the metabolites such as glucose and reactive oxygen species is of great significance for precise screening, monitoring and prognosis of metabolic disorders and relevant diseases s...
journal_title:Biotechnology advances
pub_type: 杂志文章,评审
doi:10.1016/j.biotechadv.2016.01.006
更新日期:2016-05-01 00:00:00
abstract::The review assesses current status and attempts to forecast trends in the development of lectin biorecognition technology. The progressive trend is characterized scientometrically and reflects the current transient situation, when standard low-throughput lectin-based techniques are being replaced by a novel microarray...
journal_title:Biotechnology advances
pub_type: 杂志文章,评审
doi:10.1016/j.biotechadv.2008.07.003
更新日期:2009-01-01 00:00:00
abstract::Malolactic fermentation (MLF) in wine can be accomplished by relying on the natural microflora or by inducing through inoculation of a specific strain(s) of malolactic bacteria, primarily strains of Leuconostoc oenos. Problems with inducing MLF include intrinsic factors of the grape must such as pH, presence of sulfur...
journal_title:Biotechnology advances
pub_type: 杂志文章
doi:10.1016/0734-9750(89)90179-1
更新日期:1989-01-01 00:00:00
abstract::Within five years, the CRISPR-Cas system has emerged as the dominating tool for genome engineering, while also changing the speed and efficiency of metabolic engineering in conventional (Saccharomyces cerevisiae and Schizosaccharomyces pombe) and non-conventional (Yarrowia lipolytica, Pichia pastoris syn. Komagataella...
journal_title:Biotechnology advances
pub_type: 杂志文章,评审
doi:10.1016/j.biotechadv.2018.01.006
更新日期:2018-01-01 00:00:00
abstract::This review discusses peptide-based vaccines in breast cancer, immune responses and clinical outcomes, which include studies on animal models and phase I, phase I/II, phase II and phase III clinical trials. Peptide-based vaccines are powerful neoadjuvant immunotherapies that can directly target proteins expressed in t...
journal_title:Biotechnology advances
pub_type: 杂志文章,评审
doi:10.1016/j.biotechadv.2015.10.013
更新日期:2015-12-01 00:00:00
abstract::This work reviews applications of high added value molecules produced from microalgae. Older forms of valorization - health food and quality feed, polyunsaturated fatty acids, pigments, carbohydrates - are currently penetrating their markets. They are driven by desirable properties: texturer and dye for food industry,...
journal_title:Biotechnology advances
pub_type: 杂志文章,评审
doi:10.1016/j.biotechadv.2020.107545
更新日期:2020-01-01 00:00:00
abstract::There is considerable interest in recent years in the bioconversion of forestry and agricultural residues into ethanol and value-added chemicals. High ethanol yields from lignocellulosic residues are dependent on efficient use of all the available sugars including glucose and xylose. The well-known fermentative yeast ...
journal_title:Biotechnology advances
pub_type: 杂志文章,评审
doi:10.1016/j.biotechadv.2007.04.001
更新日期:2007-09-01 00:00:00