Abstract:
:Tissue clearing and light-sheet microscopy have a 100-year-plus history, yet these fields have been combined only recently to facilitate novel experiments and measurements in neuroscience. Since tissue-clearing methods were first combined with modernized light-sheet microscopy a decade ago, the performance of both technologies has rapidly improved, broadening their applications. Here, we review the state of the art of tissue-clearing methods and light-sheet microscopy and discuss applications of these techniques in profiling cells and circuits in mice. We examine outstanding challenges and future opportunities for expanding these techniques to achieve brain-wide profiling of cells and circuits in primates and humans. Such integration will help provide a systems-level understanding of the physiology and pathology of our central nervous system.
journal_name
Neuronjournal_title
Neuronauthors
Ueda HR,Dodt HU,Osten P,Economo MN,Chandrashekar J,Keller PJdoi
10.1016/j.neuron.2020.03.004subject
Has Abstractpub_date
2020-05-06 00:00:00pages
369-387issue
3eissn
0896-6273issn
1097-4199pii
S0896-6273(20)30190-2journal_volume
106pub_type
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