Abstract:
:The combination of patch-clamp and molecular biology techniques has made it possible to characterize the pharmacological and biophysical properties of ion channels in single neurons and to screen for expression of specific mRNAs in the same cell. Following whole-cell recording, the cytoplasm of the cell is harvested, and RNA is reverse transcribed into cDNA and amplified in PCR with primers specific for individual ion channel subunits. Additional experiments can then be designed to relate structure and function at the protein level more directly, since cells appear to regulate the composition of ion channels at least partly at the posttranscriptional stage.
journal_name
Neuronjournal_title
Neuronauthors
Sucher NJ,Deitcher DLdoi
10.1016/0896-6273(95)90257-0subject
Has Abstractpub_date
1995-06-01 00:00:00pages
1095-100issue
6eissn
0896-6273issn
1097-4199pii
0896-6273(95)90257-0journal_volume
14pub_type
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