Abstract:
:The precise subunit composition of synaptic ionotropic receptors in the brain is poorly understood. This information is of particular importance with regard to AMPA-type glutamate receptors, the multimeric complexes assembled from GluA1-A4 subunits, as the trafficking of these receptors into and out of synapses is proposed to depend upon the subunit composition of the receptor. We report a molecular quantification of synaptic AMPA receptors (AMPARs) by employing a single-cell genetic approach coupled with electrophysiology in hippocampal CA1 pyramidal neurons. In contrast to prevailing views, we find that GluA1A2 heteromers are the dominant AMPARs at CA1 cell synapses (approximately 80%). In cells lacking GluA1, -A2, and -A3, synapses are devoid of AMPARs, yet synaptic NMDA receptors (NMDARs) and dendritic morphology remain unchanged. These data demonstrate a functional dissociation of AMPARs from trafficking of NMDARs and neuronal morphogenesis. This study provides a functional quantification of the subunit composition of AMPARs in the CNS and suggests novel roles for AMPAR subunits in receptor trafficking.
journal_name
Neuronjournal_title
Neuronauthors
Lu W,Shi Y,Jackson AC,Bjorgan K,During MJ,Sprengel R,Seeburg PH,Nicoll RAdoi
10.1016/j.neuron.2009.02.027subject
Has Abstractpub_date
2009-04-30 00:00:00pages
254-68issue
2eissn
0896-6273issn
1097-4199pii
S0896-6273(09)00255-4journal_volume
62pub_type
杂志文章相关文献
NEURON文献大全abstract::Transmembrane channel-like (TMC) 1 and 2 are required for the mechanotransduction of mouse inner ear hair cells and localize to the site of mechanotransduction in mouse hair cell stereocilia. However, it remains unclear whether TMC1 and TMC2 are indeed ion channels and whether they can sense mechanical force directly....
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/j.neuron.2019.10.017
更新日期:2020-01-22 00:00:00
abstract::Neuronal gain control is important for processing information in the brain. Shunting inhibition is not thought to control gain since it shifts input-output relationships during tonic excitation rather than changing their slope. Here we show that tonic inhibition reduces the gain and shifts the offset of cerebellar gra...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/s0896-6273(03)00200-9
更新日期:2003-05-08 00:00:00
abstract::Spontaneous activity patterns in the developing retina appear important for the functional organization of the visual system. We show here that an absence of early retinal waves in mice lacking the beta2 subunit of the nicotinic acetylcholine receptor (nAChR) is associated with both gain and loss of functional organiz...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/s0896-6273(03)00789-x
更新日期:2003-12-18 00:00:00
abstract::Here we define activities of RIM-binding protein (RBP) that are essential for baseline neurotransmission and presynaptic homeostatic plasticity. At baseline, rbp mutants have a ∼10-fold decrease in the apparent Ca(2+) sensitivity of release that we attribute to (1) impaired presynaptic Ca(2+) influx, (2) looser coupli...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/j.neuron.2015.01.024
更新日期:2015-03-04 00:00:00
abstract::Epitranscriptomic modification of mRNA affects its metabolism and has recently been shown to regulate brain development. Two studies in this issue of Neuron, Koranda et al. (2018) and Engel et al. (2018), uncover dynamic and critical roles of m6A/m RNA modifications in the adult mammalian brain in regulating physiolog...
journal_title:Neuron
pub_type: 评论,杂志文章
doi:10.1016/j.neuron.2018.07.019
更新日期:2018-07-25 00:00:00
abstract::Social environment modulates learning through unknown mechanisms. Here, we report that a pheromone mixture that signals overcrowding inhibits C. elegans from learning to avoid pathogenic bacteria. We find that learning depends on the balanced signaling of two insulin-like peptides (ILPs), INS-16 and INS-4, which act r...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/j.neuron.2019.09.006
更新日期:2019-12-18 00:00:00
abstract::To survive, humans must estimate their own ability and the abilities of others. We found that, although people estimated their abilities on the basis of their own performance in a rational manner, their estimates of themselves were partly merged with the performance of others. Reciprocally, their ability estimates for...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/j.neuron.2016.06.022
更新日期:2016-07-20 00:00:00
abstract::The discovery of SNCA mutations pathogenic for autosomal-dominant Lewy body Parkinson's disease (PD) in 1997 heralded a revolution in understanding the molecular and genetic basis of PD. Indeed, it now is clear that Lewy body PD is one of many neurodegenerative parkinsonian disorders that result from nigrostriatal deg...
journal_title:Neuron
pub_type: 杂志文章,评审
doi:10.1016/j.neuron.2005.07.021
更新日期:2005-08-18 00:00:00
abstract::Alzheimer's disease (AD) displays a long asymptomatic stage before dementia. We characterize AD stage-associated molecular networks by profiling 14,513 proteins and 34,173 phosphosites in the human brain with mass spectrometry, highlighting 173 protein changes in 17 pathways. The altered proteins are validated in two ...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/j.neuron.2019.12.015
更新日期:2020-03-18 00:00:00
abstract::The retina adapts to average light intensity but also to the range of light intensities (contrast). A study by Baccus and Meister, in this issue of Neuron, identifies three ways that ganglion cells and interneurons adapt to high contrast: shorten integration time, reduce gain, and depolarize. Only the depolarization d...
journal_title:Neuron
pub_type: 评论,杂志文章
doi:10.1016/s0896-6273(02)01100-5
更新日期:2002-12-05 00:00:00
abstract::We explored the relationship between regulation of the spine actin cytoskeleton, spine morphogenesis, and synapse formation by manipulating expression of the actin binding protein NrbI and its deletion mutants. In pyramidal neurons of cultured rat hippocampal slices, NrbI is concentrated in dendritic spines by binding...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/j.neuron.2004.09.022
更新日期:2004-10-14 00:00:00
abstract::We are rapidly approaching a better understanding of the mechanisms that allow our brains to form distinct representations for similar events or episodes. McHugh et al. have brought that goal one step closer by showing that NMDA receptor-dependent synaptic plasticity in the dentate gyrus is necessary for immediate dif...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/j.neuron.2007.07.002
更新日期:2007-07-19 00:00:00
abstract::Sweet and bitter taste distinguishes good food sources from potential toxins, but what happens when these tastants are mixed? In this issue of Neuron, Jeong et al. (2013) show that in Drosophila, bitter compounds act through an extracellular odorant-binding protein to inhibit sweet-responsive neurons and block the res...
journal_title:Neuron
pub_type: 评论,杂志文章
doi:10.1016/j.neuron.2013.07.038
更新日期:2013-08-21 00:00:00
abstract::Input-timing-dependent plasticity (ITDP) is a circuit-based synaptic learning rule by which paired activation of entorhinal cortical (EC) and Schaffer collateral (SC) inputs to hippocampal CA1 pyramidal neurons (PNs) produces a long-term enhancement of SC excitation. We now find that paired stimulation of EC and SC in...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/j.neuron.2017.07.036
更新日期:2017-08-30 00:00:00
abstract::The signaling mechanisms that control the survival of CNS neurons are poorly understood. Here we show that, in contrast to PNS neurons, the survival of purified postnatal rat retinal ganglion cells (RGCs) in vitro is not promoted by peptide trophic factors unless their intracellular cAMP is increased pharmacologically...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/0896-6273(95)90172-8
更新日期:1995-10-01 00:00:00
abstract::Neuroimaging studies using diffusion tensor imaging (DTI) have revealed regions of cerebral white matter with decreased microstructural organization (lowerfractional anisotropy or FA) among poor readers. We examined whether 100 hr of intensive remedial instruction affected the white matter of 8- to 10-year-old poor re...
journal_title:Neuron
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.neuron.2009.10.018
更新日期:2009-12-10 00:00:00
abstract::The targeting of sensory afferent neurons in the leech CNS occurs in two discrete steps that are mediated via different carbohydrate recognitions, as shown by molecular perturbations of cultured embryos. A constitutive carbohydrate marker that is generic to all of these neurons mediates their initial defasciculation a...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/0896-6273(95)90310-0
更新日期:1995-03-01 00:00:00
abstract::Neurotropic viruses that conditionally infect or replicate in molecularly defined neuronal subpopulations, and then spread transsynaptically, are powerful tools for mapping neural pathways. Genetically targetable retrograde transsynaptic tracer viruses are available to map the inputs to specific neuronal subpopulation...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/j.neuron.2011.12.002
更新日期:2011-12-22 00:00:00
abstract::The controversy about whether dopamine contributes to cell loss in Parkinson's disease takes a new turn as Mosharov et al. in this issue of Neuron demonstrate that Ca2+ influx through L-type channels elevates dopamine synthesis to potentially toxic levels in vulnerable ventral mesencephalon neurons. ...
journal_title:Neuron
pub_type: 评论,杂志文章
doi:10.1016/j.neuron.2009.04.010
更新日期:2009-04-30 00:00:00
abstract::Protein kinase A (PKA) is a key regulator of neurosecretion, but the molecular targets remain elusive. We combined pharmacological manipulations of kinase and phosphatase activities with mutational studies on the exocytotic machinery driving fusion of catecholamine-containing vesicles from chromaffin cells. We found t...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/s0896-6273(04)00038-8
更新日期:2004-02-05 00:00:00
abstract::We utilized the Cre-LoxP system to establish erbB2 conditional mutant mice in order to investigate the role of erbB2 in postnatal development of the enteric nervous system. The erbB2/nestin-Cre conditional mutants exhibit retarded growth, distended colons, and premature death, resembling human Hirschsprung's disease. ...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/s0896-6273(02)01128-5
更新日期:2003-01-09 00:00:00
abstract::Seizure causes a rapid and protracted increase in transcription factor AP-1 levels in the brain. The composition of AP-1 nucleoprotein complexes changes with time after seizure as a result of the sequential appearance and disappearance of Fos and several Fos-related proteins. Although these changes occur over an 8 hr ...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/0896-6273(89)90260-2
更新日期:1989-09-01 00:00:00
abstract::Lipids are prominent components of the nervous system. Here we performed a large-scale mass spectrometry-based analysis of the lipid composition of three brain regions as well as kidney and skeletal muscle of humans, chimpanzees, rhesus macaques, and mice. The human brain shows the most distinct lipid composition: 76%...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/j.neuron.2015.01.003
更新日期:2015-02-18 00:00:00
abstract::Although numerous theoretical models implicate the prefrontal cortex (PFC) as a source of top-down control signals, empirical support is limited. In this review, we highlight direct evidence for this view of PFC function and discuss several lines of other supportive findings. Most evidence to-date is only suggestive d...
journal_title:Neuron
pub_type: 杂志文章,评审
doi:10.1016/j.neuron.2005.11.002
更新日期:2005-11-23 00:00:00
abstract::Recent binding studies in the central nervous system and other tissues provide evidence that the mammalian bombesin-like peptides, gastrin-releasing peptide (GRP) and neuromedin-B (NMB), exert their numerous physiological effects through at least two different receptors. We describe the structure and expression of a c...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/0896-6273(91)90250-4
更新日期:1991-03-01 00:00:00
abstract::Incoming stimuli are encoded collectively by populations of cortical neurons, which transmit information by using a neural code thought to be predominantly redundant. Redundant coding is widely believed to reflect a design choice whereby neurons with overlapping receptive fields sample environmental stimuli to convey ...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/j.neuron.2019.07.006
更新日期:2019-10-23 00:00:00
abstract::Proteolipid protein (PLP) is an integral membrane protein of CNS myelin. Mutations of the X chromosome-linked PLP gene cause glial cell death and myelin deficiency in jimpy mice and other neurological mutants. As part of an attempt to rescue these mutants by transgenic complementation, we generated normal mouse lines ...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/0896-6273(94)90214-3
更新日期:1994-03-01 00:00:00
abstract::Auditory information critical for fear conditioning, a model of emotional learning, is conveyed to the lateral nucleus of the amygdala via two routes: directly from the medial geniculate nucleus and indirectly from the auditory cortex. Here we show in the cortico-amygdala pathway that learned fear occludes electricall...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/s0896-6273(02)00645-1
更新日期:2002-04-11 00:00:00
abstract::Self-consciousness has mostly been approached by philosophical enquiry and not by empirical neuroscientific study, leading to an overabundance of diverging theories and an absence of data-driven theories. Using robotic technology, we achieved specific bodily conflicts and induced predictable changes in a fundamental a...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/j.neuron.2011.03.009
更新日期:2011-04-28 00:00:00
abstract::Compelling evidence links amyloid beta (Aβ) peptide accumulation in the brains of Alzheimer's disease (AD) patients with the emergence of learning and memory deficits, yet a clear understanding of the events that drive this synaptic pathology are lacking. We present evidence that neurons exposed to Aβ are unable to fo...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/j.neuron.2017.09.041
更新日期:2017-10-11 00:00:00