Abstract:
:Recent advances in next-generation sequencing have enabled rapid and efficient evaluation of the mutational landscape of cancers. As a result, many cancer-specific neoantigens, which can generate antitumor cytotoxic T-cells inside tumors, have been identified. Previously, we reported a metastatic melanoma case with high tumor mutation burden, who obtained complete remission after anti-PD-1 therapy and surgical resection. The rib metastatic lesion, which was used for whole-exome sequencing and gene expression profiling in the HOPE project, showed upregulated expression of PD-L1 mRNA and a high single-nucleotide variants number of 2712. In the current study, we focused on a metastatic melanoma case and candidate epitopes among nonsynonymous mutant neoantigens of 1348 variants were investigated using a peptide-HLA binding algorithm, in vitro cytotoxic T-cell induction assay and HLA tetramer staining. Specifically, from mutant neoantigen data, a total of 21,066 9-mer mutant epitope candidates including a mutated amino acid anywhere in the sequence were applied to the NetMHC binding prediction algorithm. From in silico data, we identified the top 26 mutant epitopes with strong-binding capacity. A cytotoxic T-cell induction assay using 5 cancer patient-derived PBMCs revealed that the mutant ARMT1 peptide sequence (FYGKTILWF) with HLA-A*2402 restriction was an efficient neoantigen, which was detected at a frequency of approximately 0.04% in the HLA-A24 tetramer stain. The present success in identifying a novel mutant antigen epitope might be applied to clinical neoantigen screening in the context of an NGS-equipped medical facility for the development of the next-generation neoantigen cancer vaccines.
journal_name
Immunol Lettjournal_title
Immunology lettersauthors
Nonomura C,Otsuka M,Kondou R,Iizuka A,Miyata H,Ashizawa T,Sakura N,Yoshikawa S,Kiyohara Y,Ohshima K,Urakami K,Nagashima T,Ohnami S,Kusuhara M,Mitsuya K,Hayashi N,Nakasu Y,Mochizuki T,Yamaguchi K,Akiyama Ydoi
10.1016/j.imlet.2019.02.004subject
Has Abstractpub_date
2019-04-01 00:00:00pages
52-59eissn
0165-2478issn
1879-0542pii
S0165-2478(18)30488-7journal_volume
208pub_type
杂志文章abstract::Recombinant and pure "natural" IL-1 and IL-2 were compared with the muramyl dipeptide (MDP) component of Freund's adjuvant for their capacity to enhance the humoral immune response against foot-and-mouth disease (FMD) virus antigen. Using a dose of this antigen which alone did not give a detectable immune response, an...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(92)90008-c
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journal_title:Immunology letters
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journal_title:Immunology letters
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journal_title:Immunology letters
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journal_title:Immunology letters
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journal_title:Immunology letters
pub_type: 杂志文章
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journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(90)90031-k
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journal_title:Immunology letters
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journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(94)90098-1
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journal_title:Immunology letters
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journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(91)90084-n
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journal_title:Immunology letters
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journal_title:Immunology letters
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journal_title:Immunology letters
pub_type: 杂志文章
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journal_title:Immunology letters
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更新日期:2000-03-01 00:00:00
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journal_title:Immunology letters
pub_type: 杂志文章
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更新日期:2000-07-03 00:00:00
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journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2012.02.007
更新日期:2012-03-30 00:00:00
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journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/s0165-2478(97)00153-3
更新日期:1998-02-01 00:00:00