Abstract:
:Galectin-1 (Gal-1) has been implicated in tumor progression partly via the induction of T-cell apoptosis. However the mechanism of Gal-1 induced T-cell death was mostly studied using recombinant, soluble Gal-1 producing controversial results. To explore the true mechanism of Gal-1 and hence tumor cell-induced T-cell death, we applied co-cultures of tumor cells and T-cells thus avoiding artificial circumstances generated using recombinant protein. T-cells died when co-cultured with Gal-1-expressing but survived with Gal-1 non-expressing tumor cells. Removing tumor cell surface Gal-1 or knocking down Gal-1 expression resulted in diminution of T-cell apoptosis. Gal-1 transgenic or soluble Gal-1 treated HeLa cells became cytotoxic. Stimulation of apoptosis required interaction between the tumor and T-cells, presence of p56lck and ZAP70, decrease of mitochondrial membrane potential and caspase activation. Hence tumor cell-derived Gal-1 might efficiently contribute to tumor self-defense. Moreover this system resolves the discrepancies obtained using recombinant Gal-1 in T-cell apoptosis studies.
journal_name
Immunol Lettjournal_title
Immunology lettersauthors
Kovács-Sólyom F,Blaskó A,Fajka-Boja R,Katona RL,Végh L,Novák J,Szebeni GJ,Krenács L,Uher F,Tubak V,Kiss R,Monostori Edoi
10.1016/j.imlet.2009.10.003subject
Has Abstractpub_date
2010-01-04 00:00:00pages
108-18issue
2eissn
0165-2478issn
1879-0542pii
S0165-2478(09)00247-8journal_volume
127pub_type
杂志文章abstract::Two cell lines producing monoclonal antibodies have been established from peripheral blood of a negative Rhesus blood donor which has been immunized with positive Rhesus red blood cells. Two monoclonal antibodies Co II 8.8 and Co II 7.12 have been selected. Both are IgG1 antibodies, but recognize different epitopes on...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(86)90046-5
更新日期:1986-09-01 00:00:00
abstract::In human tumors of neuroectodermal origin cell surface expression of individual gangliosides is either increased or decreased relative to comparable normal cells. We have previously shown that gangliosides shed from melanoma cells can immunomodulate T cell activity. Monocytes/macrophages (m/m) are known to play an imp...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(89)90034-5
更新日期:1989-03-01 00:00:00
abstract::CD24 is an extensively glycosylated membrane protein that is linked to the membrane via a glycosyl-phosphatidylinositol (GPI)-anchor. In mice, CD24 is expressed by hematopoietic and non-hematopoietic cells. CD24-/- mice do not have gross immunological defects, but detailed analysis revealed strongly reduced responses ...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2014.06.004
更新日期:2014-09-01 00:00:00
abstract::Peanut agglutinin (PNA), a D-galactose specific lectin, agglutinated 10-18% of lymphocytes isolated from tonsils of 3- to 6-yr-old children. PNA+ cells were found to be mainly B lymphocytes showing a 9.6 times higher specific activity of DNA polymerase alpha compared to the PNA- cells. The specific activity of deoxycy...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(87)90040-x
更新日期:1987-06-01 00:00:00
abstract::This study aimed to investigate the influence of physical activity in innate immunity to conduce to an effective antitumoral immune response analyzing the phenotype and activation status of infiltrating cells. We analysed the intracellular cytokines and the transcription factors of tumor infiltrating lymphocytes (TILS...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2017.08.007
更新日期:2017-10-01 00:00:00
abstract::Human deficiency in transporter associated with antigen processing (TAP) is characterized by a very low surface expression of human leukocyte antigen (HLA) class I molecules in hematopoietic and non hematopoietic cells. Among the latter, TAP-deficient skin fibroblasts have previously been shown by us to be very sensit...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2006.07.010
更新日期:2006-11-15 00:00:00
abstract::African swine fever (ASF) virus has been reported to infect cells of the monocyte family, probably macrophage-like cells, but there is variation in the apparent susceptibility of these cells. We have demonstrated that the phenotype and activity of porcine monocytic cells varies between different isolations and also up...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(93)90136-p
更新日期:1993-07-01 00:00:00
abstract::We have previously reported the production of a series of human monoclonal antibodies reacting with mouse mammary tumor viral antigens as well as the human counterpart HuMTV antigen. Against two of these antibodies (B11 and 4.6/6), anti-idiotypic antibodies were generated, which appeared to be the internal image of vi...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(91)90128-w
更新日期:1991-04-01 00:00:00
abstract::Luminol-dependent chemiluminescence (CL) triggered by mineral dust in rabbit alveolar macrophages depended on superoxide production as revealed by inhibition by superoxide dismutase and ferricytochrome c. The contribution of superoxide changed in the course of CL peak between 80-20%. The effects of ferricytochrome c w...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(87)90137-4
更新日期:1987-08-01 00:00:00
abstract::Complement factor I (CFI) deficiency is typically associated to recurrent infections with encapsulated microorganisms and, less commonly, to autoimmunity. We report a 53-years old male who, in a routine control for non-alcoholic fatty liver disease, presented a flat beta-2 fraction at the capillary protein electropher...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2016.04.011
更新日期:2016-06-01 00:00:00
abstract::Wnt5a is a secreted pleiotropic glycoprotein produced in an inflammatory state by a wide spectrum of ubiquitous cell populations. Recently, we demonstrated that Wnt5a skews the differentiation of human monocyte derived dendritic cells (moDCs) to a tolerogenic functional state. In this study we focus our interest on th...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2014.08.015
更新日期:2014-11-01 00:00:00
abstract::Several immunodominant B-cell epitopes of the P. falciparum antigen blood stage Pf155/RESA, a major vaccine candidate antigen, are located in the molecular regions containing amino acid repeats. We started to map Pf155/RESA for T cell reactive epitopes. For this purpose, short synthetic peptides corresponding to the 3...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(88)90147-2
更新日期:1988-11-01 00:00:00
abstract::Clone C19 derived from a rat bladder cancer cell line BC31ad constitutively released cytotoxic factor RCF. The serum-free C19-conditioned medium was partially purified by DEAE-Sephacel, Sephacryl S-200 and MonoQ-FPLC chromatographies to the specific activity of 6.4 x 10(6) U/mg protein (LM assay). RCF was a 17-kDa pro...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(94)90051-5
更新日期:1994-06-01 00:00:00
abstract:BACKGROUND:Notch pathway is highly conserved across species and is involved in the regulation of cell differentiation and activity both in embryonic development and adult life. Notch signaling has an important role in the development of hematopoietic stem cells and their differentiation to committed lineages, as well a...
journal_title:Immunology letters
pub_type: 杂志文章,评审
doi:10.1016/j.imlet.2020.04.010
更新日期:2020-07-01 00:00:00
abstract::E1, E3-deleted, replication-deficient recombinant adenoviruses are widely studied as vectors for their capacity to transfer therapeutic genes in vivo. They can infect a wide variety of dividing and quiescent cells from different organs and possess a large packaging capacity. One of the major limitations in the use of ...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/s0165-2478(97)00049-7
更新日期:1997-06-01 00:00:00
abstract::A considerable body of evidence from various laboratories indicates that specific T cell responses generated during infection with Leishmania parasites play an important role both in the resolution and progression of cutaneous leishmaniasis. Recent data, summarized in this article, indicate that resolution of lesions ...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(88)90151-4
更新日期:1988-11-01 00:00:00
abstract::Between the ages of 2 to approximately 11 days mice respond to a challenge with syngeneic IgE by producing anti-IgE antibodies; by the age of 2 weeks they are unresponsive. Even adult mice, however, produce high titers of anti-IgE antibodies when immunized with a conjugate of syngeneic IgE and a foreign antigen such a...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(95)00087-3
更新日期:1995-09-01 00:00:00
abstract::Cell-surface 5'-nucleotidase was assayed by incubating intact cells with 5' [3H]AMP in iso-osmotic buffer and measuring [3H]adenosine production. The activity of cell surface 5'-nucleotidase in small resting lymphocytes and in cells of the B cell line BCL1 was 5.7 and 1.1 nmol/min/mg protein respectively at 37 degrees...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(88)90122-8
更新日期:1988-09-01 00:00:00
abstract::Satratoxins have been recognized as potential immunomodulatory agents in outbreaks of building-related illness. Here we report that satratoxin G-treated human leukemia HL-60 cells underwent apoptosis through the action of caspase-3 which was activated by both caspase-8 and caspase-9. Western blot analysis of caspase-3...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/s0165-2478(02)00127-x
更新日期:2002-10-21 00:00:00
abstract::It has been considered before that human naive and memory/effector CD4+ T-cells cannot be subdivided solely according to the differential expression of CD45 isoforms. By the lack of expression of CD31 we have identified a subset of CD4+ CD45RA+ CD31- cells which show distinct features of antigen-experienced Th1 cells....
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/s0165-2478(97)00056-4
更新日期:1997-06-01 00:00:00
abstract::Neuraminidases act as a virulence factors for several pathogens that invade the human body through Peyer's patch M-cells. Because of the structural similarity of Aleuria aurantia lectin (AAL) to neuraminidases, we hypothesized that AAL might also target human M-cells. In an in vitro human M-cell co-culture model signi...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2005.03.020
更新日期:2005-09-15 00:00:00
abstract::Work done in our laboratories, using a murine model, indicates that suppression of host immune responses might be due to secretion of soluble factors by tumor cells. The H238 cells (BALB/c embryonic fibroblasts transformed by UV-inactivated herpes simplex virus Type 2) exhibit progressive tumor growth with subsequent ...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(91)90087-q
更新日期:1991-09-01 00:00:00
abstract:UNLABELLED:To study the pathogenic importance of the rheumatoid factor (RF) in rheumatoid arthritis (RA) and to identify genes differentially expressed in patients and healthy individuals, total RNA was isolated from peripheral blood mononuclear cells (PBMC) from eight RF-positive and six RF-negative RA patients, and s...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2004.03.018
更新日期:2004-05-15 00:00:00
abstract::Two crucial mediators of monocyte activation by lipopolysaccharide (LPS) are the acute phase plasma factor, lipopolysaccharide binding protein (LBP) and cell-surface-expressed CD14. Whether macrophage (M phi) recognized and respond to LPS in a similar manner is unknown. Here we show that human monocyte-derived M phi r...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/s0165-2478(96)02645-4
更新日期:1996-12-01 00:00:00
abstract::The possibility of inducing immune responses to murine interleukin-4 (IL-4) and IL-13 and generating anti-cytokine monoclonal antibodies (mAbs) was studied in IL-4- and IL-13-knockout mice. The minimal doses of IL-4 or IL-13 that could induce significant anti-cytokine responses with titers of 5000-10,000 were 20 micro...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2005.08.005
更新日期:2006-02-15 00:00:00
abstract::Secondary lymphoid organs (SLO) are crucial structures for immune-surveillance and rapid immune responses allowing resident lymphocytes to encounter antigen-presenting cells that carry antigens from peripheral tissues. These structures develop during embryonic life through a tightly regulated process that involves int...
journal_title:Immunology letters
pub_type: 杂志文章,评审
doi:10.1016/j.imlet.2013.08.001
更新日期:2013-11-01 00:00:00
abstract::In the present investigations we aimed to study the effect of Shigella sonnei lipopolysaccharide (LPS) on delayed type hypersensitivity (DTH) to non-bacterial antigen in CBA mice. These experiments showed that intraperitoneal injection of phenol-water extracted LPS and avirulent S. sonnei did not affect the level of D...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/s0165-2478(01)00298-x
更新日期:2002-01-01 00:00:00
abstract::The induction of antigen (Ag)-specific tolerance is critical for the prevention of autoimmunity and maintenance of immune homeostasis. Recently, attention has been focused on induction of active suppression by regulatory T cells (Treg cells). Also, a role for dendritic cells (DCs) in the promotion of peripheral tolera...
journal_title:Immunology letters
pub_type: 杂志文章,评审
doi:10.1016/j.imlet.2004.04.015
更新日期:2004-06-15 00:00:00
abstract::N-Chlorotaurine (NCT) plays an important role in the human defense system as a main component of long-lived oxidants, and shows bactericidal, fungicidal, and virucidal activity. Besides this role, NCT seems to act regulatory on immunocompetent cells by altering cytokine production. NCT inhibited nitric oxide, TNF-alph...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2004.03.006
更新日期:2004-05-15 00:00:00
abstract::The effect of melphalan (L-PAM; L-phenylalanine mustard) administration on natural killer (NK) and natural cytotoxic (NC) activities of spleen cells from normal noninoculated BALB/c mice and mice bearing large MOPC-315 plasmacytoma tumors was investigated. Injection of L-PAM (7.5 or 15.0 mg/kg) had no effect on NK and...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(85)90128-2
更新日期:1985-01-01 00:00:00