Abstract：
:Many types of cells in the immune system have been found to produce nitric oxide (NO), which performs multiplex functions. However, in myelin basic protein peptide 68-86 (MBP 68-86)-induced experimental autoimmune encephalomyelitis (EAE) in Lewis rats, we found that elevated NO production was generated from spleen cells (SC), not from lymph node cells (LNC). LNC expressed lower NO synthase 2 (NOS2) and produced lower levels of NO than SC upon MBP 68-86 stimulation. Expression of B7-1(CD80) and B7-2(CD86) molecules was much lower on LNC than on SC. Blocking of B7-1 or B7-2 ligation resulted in reduced NO production by SC. Unlike SC, LNC were resistant to interferon-gamma- or lipopolysaccharide-induced NO production. NO derived from SC suppressed cell proliferation and induced apoptosis in vitro. Addition of N(omega)-nitrol-L-arginine methylester (L-NAME) into cell cultures promoted cell expansion and reduced apoptosis. These results indicate that NO production originates from SC, but not from LNC. Low expression of co-stimulatory molecules and NOS2 of LNC limits NO induction. The high levels of NO derived from SC are involved in the self-limiting mechanisms of autoimmune responses by inhibiting cell expansion and promoting cell apoptosis.
journal_name
Immunol Lettjournal_title
Immunology lettersauthors
Xu L,Yang J,Huang Y,van der Meide PH,Levi M,Wahren B,Link H,Xiao Bdoi
10.1016/s0165-2478(00)00154-1subject
Has Abstractpub_date
2000-03-01 00:00:00pages
177-84issue
3eissn
0165-2478issn
1879-0542pii
S0165247800001541journal_volume
71pub_type
杂志文章abstract：AIM:To develop new approach for tumor immunotherapy with VEGFR2-based DNA vaccine. METHODS:VEGFR2 derived from different species were prepared by RT-PCR and subjected to recombinant constructs. The chimeric gene of VEGFR2 was prepared by integrating different epitopes in different species. By transfecting into cells, ...
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pub_type： 杂志文章
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更新日期：1991-10-01 00:00:00
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journal_title：Immunology letters
pub_type： 杂志文章
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journal_title：Immunology letters
pub_type： 杂志文章
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更新日期：1996-01-01 00:00:00
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journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/0165-2478(94)90183-x
更新日期：1994-05-01 00:00:00
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journal_title：Immunology letters
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更新日期：2009-06-30 00:00:00
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journal_title：Immunology letters
pub_type： 杂志文章,评审
doi：10.1016/j.imlet.2020.02.009
更新日期：2020-05-01 00:00:00
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journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/j.imlet.2014.09.007
更新日期：2015-02-01 00:00:00
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journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/j.imlet.2004.10.011
更新日期：2005-02-15 00:00:00
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journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/0165-2478(92)90004-8
更新日期：1992-01-01 00:00:00
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journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/0165-2478(95)00034-3
更新日期：1995-05-01 00:00:00
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更新日期：1992-06-01 00:00:00
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journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/j.imlet.2014.03.009
更新日期：2014-07-01 00:00:00
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journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/j.imlet.2005.03.016
更新日期：2005-09-15 00:00:00
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更新日期：2019-06-01 00:00:00
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pub_type： 杂志文章
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更新日期：1990-08-01 00:00:00
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journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/0165-2478(94)90122-8
更新日期：1994-07-01 00:00:00
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更新日期：2012-12-17 00:00:00
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journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/0165-2478(92)90110-a
更新日期：1992-04-01 00:00:00
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更新日期：2009-08-15 00:00:00
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journal_title：Immunology letters
pub_type： 杂志文章
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更新日期：1995-05-01 00:00:00
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journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/s0165-2478(02)00290-0
更新日期：2003-03-03 00:00:00
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journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/0165-2478(87)90155-6
更新日期：1987-12-01 00:00:00
