Abstract:
:The observation that protective immunity induced by immunization with radiation attenuated Plasmodium berghei and Plasmodium yoelii sporozoites is dependent on CD8+ T lymphocytes in some strains of mice led us to speculate that immunization with sporozoites induces cytotoxic T lymphocytes (CTL) that recognize malaria antigens on the surface of malaria-infected hepatocytes. In this report we summarize a series of experiments that confirm this hypothesis. We first showed that when immune mice are challenged with live sporozoites they develop malaria-specific, CD8+ T cell-dependent infiltrates in their livers. Next we demonstrated that spleen cells from immune mice eliminate malaria infected hepatocytes from in vitro culture in an antigen specific and genetically restricted manner, indicating that these immune cells recognize malaria antigens on the surface of infected hepatocytes. Finally we defined a CTL epitope of the P. yoelii CS protein, and demonstrated that CTL against this 16-amino-acid peptide (PYCTL1) eliminate infected hepatocytes from culture in an antigenic specific, and MHC restricted manner, indicating that this 16-amino-acid peptide from the CS protein is present on the surface of the infected hepatocytes. We are currently working on constructing vaccines that induce protective CTL against PYCTL1, and identifying additional pre-erythrocytic stage targets of CTL mediated protective immunity.
journal_name
Immunol Lettjournal_title
Immunology lettersauthors
Hoffman SL,Weiss W,Mellouk S,Sedegah Mdoi
10.1016/0165-2478(90)90087-7subject
Has Abstractpub_date
1990-08-01 00:00:00pages
33-8issue
1-3eissn
0165-2478issn
1879-0542pii
0165-2478(90)90087-7journal_volume
25pub_type
杂志文章abstract::Four murine monoclonal antibodies (mAbs) showing similar reactivity with a cell-wall extract and mannan preparation obtained from Candida albicans were examined for epitope specificity. An enzyme-linked immunosorbent assay (ELISA) was used to determine total mAb binding to extracted cell-wall antigen when each mAb was...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(89)90134-x
更新日期:1989-07-01 00:00:00
abstract:BACKGROUND:The autoimmune regulator (AIRE) regulates autoimmunity and self-antigen expression, such as acetylcholine receptor (AchR), in the thymus. Regulatory T cells (Tregs) can down-regulate autoimmunity, but also promote tumor growth. The objective of this study was to examine the levels of AIRE, AchR, and Foxp3 ex...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2014.04.008
更新日期:2014-09-01 00:00:00
abstract::Salmonella enterica serovars infect a broad range of mammalian hosts, including humans, causing both gastrointestinal and systemic diseases. Effective immune responses to Salmonella infections depend largely on CD4+ T cell activation by dendritic cells (DCs). Bacteria are internalised by intestinal DCs and respond by ...
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pub_type: 杂志文章,评审
doi:10.1016/j.imlet.2019.01.006
更新日期:2019-11-01 00:00:00
abstract::The induction of antigen (Ag)-specific tolerance is critical for the prevention of autoimmunity and maintenance of immune homeostasis. Recently, attention has been focused on induction of active suppression by regulatory T cells (Treg cells). Also, a role for dendritic cells (DCs) in the promotion of peripheral tolera...
journal_title:Immunology letters
pub_type: 杂志文章,评审
doi:10.1016/j.imlet.2004.04.015
更新日期:2004-06-15 00:00:00
abstract::CD70, the ligand of CD27, is a member of the TNF family, which includes molecules essential in the regulation of lymphocyte growth and survival. It is absent on resting lymphocytes but can be induced in vitro with activating stimuli. To extend information about its expression by different T-cell subpopulations, and it...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/s0165-2478(96)02693-4
更新日期:1997-02-01 00:00:00
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journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2007.02.005
更新日期:2007-04-15 00:00:00
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journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/s0165-2478(99)00056-5
更新日期:1999-06-01 00:00:00
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journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2009.12.018
更新日期:2010-02-16 00:00:00
abstract::The experimental infection of macaques with simian immunodeficiency virus (SIV) has provided strong evidence that it may be possible to develop a vaccine against AIDS. Live attenuated SIV vaccines have been found to confer the most potent protection against challenge with a variety of pathogenic viruses. This article ...
journal_title:Immunology letters
pub_type: 杂志文章,评审
doi:10.1016/s0165-2478(98)00153-9
更新日期:1999-03-01 00:00:00
abstract::Recent data have shown that piperlongumine (PL), an important component of Piper longum fruits, is known to possess anti-inflammatory and vascular-protective activities. This study aimed to examine the therapeutic effects and underlying mechanisms of PL on lupus-prone MRL-Fas(lpr) mice. Female MRL-Fas(lpr) mice were i...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2014.05.001
更新日期:2014-09-01 00:00:00
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journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(86)90035-0
更新日期:1986-06-01 00:00:00
abstract::In human tumors of neuroectodermal origin cell surface expression of individual gangliosides is either increased or decreased relative to comparable normal cells. We have previously shown that gangliosides shed from melanoma cells can immunomodulate T cell activity. Monocytes/macrophages (m/m) are known to play an imp...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(89)90034-5
更新日期:1989-03-01 00:00:00
abstract::The monoclonal antibody (mAb) UN2 was generated upon immunization of a Balb/c mouse with human thymocytes. mAb UN2 recognized an antigen expressed by a subpopulation of human thymocytes and peripheral blood lymphocytes. In thymus, mAb UN2 recognized cortical cells; its expression was higher on CD3bright than on CD3dim...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(94)90035-3
更新日期:1994-09-01 00:00:00
abstract::This paper describes a new murine monoclonal antibody, UN5, raised against human thymocytes. This antibody recognizes a molecule of approximately 45 kDa on thymocytes. Flow cytometric analysis reveals a high intensity of labeling with the majority of thymocytes, whereas only CD20+ cells from peripheral whole-blood sam...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(94)90098-1
更新日期:1994-02-01 00:00:00
abstract::Abnormal activation of the transcriptional factor STAT3 (signal transducer and activator of transcription 3) was recently associated with Alzheimer Disease (AD). STAT3 phosphorylation is critical for cytokine secretion linked to neuroinflammation. Moreover, STAT3 may act as a transcriptional regulator of BACE1 (β-APP ...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2020.10.004
更新日期:2020-12-01 00:00:00
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journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/s0165-2478(02)00198-0
更新日期:2002-12-03 00:00:00
abstract::Scid mice develop human EBV-positive B cell tumors after injection with EBV-transformed human B cells or peripheral blood lymphocytes from EBV-seropositive donors. Injection of cytotoxic T cell (CTL) lines, selectively expanded in vitro to recognize autologous EBV-transformed B cells, protected mice against developing...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(96)02602-8
更新日期:1996-09-01 00:00:00
abstract::We have reported that a cell line, KML1, obtained from lymph node cells of a 16-week-old female MRL/Mp-lpr/lpr (MRL/1) mouse produces a soluble factor(s) that promotes formation of anti-single-stranded (ss) DNA antibodies by cultures of spleen cells from old MRL/l mice (greater than 16 weeks old). Detailed examination...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(90)90035-o
更新日期:1990-03-01 00:00:00
abstract::The early thymic precursor population of adult mice (low CD4 precursor) has the potential to produce T cells, B cells and dendritic cells if transferred into the appropriate inductive environment of an irradiated recipient. To assess its developmental potential in vitro, this population was isolated and cultured, alon...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(95)00059-e
更新日期:1995-07-01 00:00:00
abstract::Rheumatic fever (RF) and chronic rheumatic heart disease (RHD) are complications of oropharyngeal infection caused by Streptococcus pyogenes. Despite the importance of the complement system against infections and autoimmunity diseases, studies on the role of the lectin pathway in RF and RHD are scarce. Thus, our aim w...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2020.11.006
更新日期:2021-01-01 00:00:00
abstract::Nervous and immune systems share specific recognition molecules for signals that originate in both systems. The information substances are polypeptides and their receptors. They comprise the multi-directional information exchange network whereby brain and nervous system function including mood and emotion can be integ...
journal_title:Immunology letters
pub_type: 杂志文章,评审
doi:10.1016/0165-2478(87)90171-4
更新日期:1987-12-01 00:00:00
abstract::Tuberculosis (TB) is a leading cause of global mortality due to infectious diseases. Expression of cyclooxygenase-2 (COX-2) acts as an important influencing factor favoring bacillary survival during TB infection. In this study, we investigated the Mycobacterium tuberculosis proteins recognized by sera from TB patient ...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2014.05.013
更新日期:2014-09-01 00:00:00
abstract::A theoretical analysis of experimental data is presented in this mini-review on non-random homo- and hetero-associations of cell surface receptors, which can be recruited in the plasma membrane or at the surface of the rough endoplasmic reticulum during the protein synthesis. In the latter case, the likely genetic ori...
journal_title:Immunology letters
pub_type: 杂志文章,评审
doi:10.1016/s0165-2478(02)00024-x
更新日期:2002-06-03 00:00:00
abstract::The immunosuppressant rapamycin is known to be a potent inhibitor of cytokine driven proliferation of T-lymphocytes and other cell types. Here we have examined the effect of rapamycin on the myeloblastic cell line M1 which responds to interleukin-6 (IL-6) with growth arrest and monocytoid differentiation. Single-agent...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/s0165-2478(97)00110-7
更新日期:1997-11-01 00:00:00
abstract::A murine monoclonal antibody (IgG) has been generated that binds to DNA modified with osmium tetroxide in the presence of 2,2'-bipyridine and does not interact with unmodified DNA. Reactivity of the antibody was tested by gel retardation assay, ELISA, dot-binding assay and immunoblotting. The results obtained suggest ...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(91)90084-n
更新日期:1991-09-01 00:00:00
abstract::Dendritic cells (DCs) differentiated in the presence of IL-10 preferentially induce regulatory T-cells and tolerance. Whether the tolerogenic properties displayed by these DCs (Tol-DCs) can be overcome has not been fully explored. Here we show for the first time that Tol-DCs express higher levels of TLR5 mRNA, but not...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2009.06.007
更新日期:2009-08-15 00:00:00
abstract::Receptor-binding by the B-subunit of Escherichia coli heat-labile enterotoxin (EtxB) induces apoptosis of peripheral CD8(+), but not CD4(+) T-cells. Given that peripheral CD8(+) and CD4(+) T cells arise from a common developmental pathway in the thymus, we investigated the effects of EtxB on different thymocyte popula...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/s0165-2478(03)00049-x
更新日期:2003-07-03 00:00:00
abstract::The effect of melphalan (L-PAM; L-phenylalanine mustard) administration on natural killer (NK) and natural cytotoxic (NC) activities of spleen cells from normal noninoculated BALB/c mice and mice bearing large MOPC-315 plasmacytoma tumors was investigated. Injection of L-PAM (7.5 or 15.0 mg/kg) had no effect on NK and...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(85)90128-2
更新日期:1985-01-01 00:00:00
abstract::Several immunological functions of B and T cells including IL-2 receptor expression on T cells were measured in 12-month-old Fisher-344 male rats maintained from 6 weeks of age on an ad libitum (AL) or a 40% food-restricted (FR) diet. Direct anti-SRBC plaque-forming cell (PFC) assays revealed a higher response in FR r...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(89)90124-7
更新日期:1989-12-01 00:00:00
abstract::The antischistosomal property of a short course of cyclosporin A (CsA), administered at the time of infection was demonstrated in the rat. In comparison with vehicle-treated controls, no schistosomes were recovered from CsA-treated animals, 18-19 days post infection. Flow cytometric analysis of blood and spleen lympho...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(88)90086-7
更新日期:1988-02-01 00:00:00