Abstract:
:The early thymic precursor population of adult mice (low CD4 precursor) has the potential to produce T cells, B cells and dendritic cells if transferred into the appropriate inductive environment of an irradiated recipient. To assess its developmental potential in vitro, this population was isolated and cultured, alone and with various stromal cell lines. Cultured alone, these precursor cells all died rapidly. Co-culture with 3T3 fibroblasts gave good survival but no growth. Co-culture with thymic cortical epithelial cell lines induced significant proliferation after an initial 50% cell loss. However, the supernatant of these cortical epithelial cell lines caused only limited proliferation after very extensive cell death. Examination of the surface phenotype of the cultures on the cortical epithelial layer showed some changes which were compatible with very early steps of thymocyte development, but none of the features of more developed T cells were seen. A proportion of the proliferating cells developed some of the surface markers and morphology of dendritic cells. Immature myeloid cells also grew in these cultures; these appeared to derive from a small number of myeloid progenitors, possibly contaminants within the preparation, and their outgrowth required only soluble factors released by the cortical epithelial cells.
journal_name
Immunol Lettjournal_title
Immunology lettersauthors
Saunders DJ,Georgiou HM,Wu L,Shortman Kdoi
10.1016/0165-2478(95)00059-esubject
Has Abstractpub_date
1995-07-01 00:00:00pages
45-51issue
1-2eissn
0165-2478issn
1879-0542pii
0165-2478(95)00059-Ejournal_volume
47pub_type
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journal_title:Immunology letters
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journal_title:Immunology letters
pub_type: 杂志文章
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更新日期:2013-07-01 00:00:00
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pub_type: 杂志文章
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更新日期:2003-02-03 00:00:00
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journal_title:Immunology letters
pub_type: 杂志文章
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journal_title:Immunology letters
pub_type: 杂志文章
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更新日期:1985-01-01 00:00:00
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journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/s0165-2478(00)00314-x
更新日期:2001-02-01 00:00:00
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journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/s0165-2478(98)00060-1
更新日期:1998-10-01 00:00:00
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journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2019.11.010
更新日期:2020-02-01 00:00:00
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journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2008.01.009
更新日期:2008-05-15 00:00:00
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journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(94)00059-x
更新日期:1994-06-01 00:00:00
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pub_type: 杂志文章
doi:10.1016/j.imlet.2018.10.011
更新日期:2018-09-01 00:00:00
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journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/s0165-2478(99)00166-2
更新日期:2000-01-10 00:00:00
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journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2005.05.012
更新日期:2005-11-15 00:00:00
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journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(94)90073-6
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:1997-03-01 00:00:00
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journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2005.02.015
更新日期:2005-06-15 00:00:00
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pub_type: 杂志文章
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journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/s0165-2478(98)00014-5
更新日期:1998-04-01 00:00:00
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journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2004.10.011
更新日期:2005-02-15 00:00:00
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journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2006.06.001
更新日期:2006-08-15 00:00:00
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journal_title:Immunology letters
pub_type: 杂志文章,评审
doi:10.1016/j.imlet.2003.10.012
更新日期:2004-03-29 00:00:00
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journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(94)90166-x
更新日期:1994-03-01 00:00:00
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journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(93)90010-y
更新日期:1993-11-01 00:00:00