Abstract:
:Several recent studies have shown that the antigen receptors (mIgM and mIgD) on normal B cell surfaces are part of larger multimolecular complexes, as each is noncovalently coupled to at least two other proteins whose phosphorylation is inducible upon G protein activation. However, B cell receptor complexes appear to be extremely labile, so that extraction of membrane immunoglobulin (mIg) from cell lysates in physical association with noncovalently coupled proteins has been shown to be highly dependent on the choice of detergent. Recent progress has to a large extent been dependent upon the introduction of digitonin, a detergent in which B cell receptor complexes are at least partially stable, as an agent to solubilize B cell membranes. Studies presented here demonstrate that if another well known detergent, octyl-beta-D-glucopyranoside (octyl glucoside), is utilized in place of digitonin, mIg receptors are immunoprecipitated from B cell lysates in association with several newly identified proteins, whose phosphorylation is also G protein dependent. In particular we find three to four mIg associated phosphoproteins in the molecular weight range of 47 to 57 kDa, and at least two others at 82 and 112 kDa. Some of these proteins may correspond to polypeptides that have been functionally linked to mIg dependent signal transduction, but have not as yet been shown to be physically coupled to the receptor.
journal_name
Immunol Lettjournal_title
Immunology lettersauthors
Chang CC,Alhasan S,Rosenspire AJdoi
10.1016/0165-2478(92)90050-xsubject
Has Abstractpub_date
1992-05-01 00:00:00pages
193-200issue
3eissn
0165-2478issn
1879-0542pii
0165-2478(92)90050-Xjournal_volume
32pub_type
杂志文章abstract::Adult T-cell leukemia-derived factor (ADF)/human thioredoxin (TRX) has thiol-dependent reducing activities and is known to have regulatory roles on the DNA-protein interaction and cell activation. Inducive effect of ultraviolet (UV) has been indicated because of the enhanced expression of ADF/TRX in epidermal cells of...
journal_title:Immunology letters
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doi:10.1016/0165-2478(95)00213-o
更新日期:1995-01-01 00:00:00
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journal_title:Immunology letters
pub_type: 杂志文章
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更新日期:2003-10-31 00:00:00
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journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2013.10.002
更新日期:2013-11-01 00:00:00
abstract::Natural anti-cholesterol antibodies (ACHAs) exist in mammalian species, moreover their level sensitively changes in pathological situations, such as atherosclerosis or HIV infection. The conditions of their production and functional role, however, still remained ill defined. Recently we developed IgG3 type monoclonal ...
journal_title:Immunology letters
pub_type: 杂志文章
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更新日期:2012-03-30 00:00:00
abstract::The monoclonal antibody (mAb) AHT-107 recognized a determinant distal to the interleukin 2 (IL-2) binding site on the p55 subunit of the IL-2 receptor (IL-2R) (the Tac antigen, CD25) of human T lymphoblasts, while the mAb AHT-54 recognized a determinant close to the IL-2 binding site as did the anti-Tac. The AHT-107 i...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(89)90081-3
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abstract::The ability of immune checkpoint inhibitors (ICIs) to reactivate the killing function of the immune system to tumor cells has led to long lasting immune response presenting highly promising clinical advances. Recently, immune checkpoint inhibitors related resistance due to the specialized tumor microenvironment has al...
journal_title:Immunology letters
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更新日期:2020-04-01 00:00:00
abstract::The present study was conducted to investigate the mode of cell death of bone marrow cells (BMC) treated in vivo or in vitro with gangliosides (DLG) derived from a T cell lymphoma, designated as Dalton's lymphoma (DL). BMC undergoing cell death showed morphological features characteristic to apoptosis such as cell shr...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/s0165-2478(00)00156-5
更新日期:2000-04-03 00:00:00
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journal_title:Immunology letters
pub_type: 杂志文章,评审
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更新日期:2013-02-01 00:00:00
abstract::We describe the quantification of the absolute amounts of HLA class II mRNA and class II transactivator (CIITA) mRNA by competitive reverse transcription polymerase chain reaction in cultured synovial fibroblast-like cells (SFC) of patients with rheumatoid arthritis. High basal levels of transcription of class II mRNA...
journal_title:Immunology letters
pub_type: 杂志文章
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更新日期:1997-06-01 00:00:00
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journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/s0165-2478(99)00056-5
更新日期:1999-06-01 00:00:00
abstract::Allogeneic, H-2-incompatible irradiation chimeras (H-2d leads to H-2b) constructed with normal, unmanipulated bone marrow and with marrow-derived factors live long and do not manifest a GvH disease. Their response to primary immunization is deficient but their alloreactivity is normal. This chimeric allotolerance cann...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(83)90003-2
更新日期:1983-04-01 00:00:00
abstract::Many alterations of innate and adaptive immunity are common in the aging population, which reflect a deterioration of the immune system, and have lead to the terms "immune aging" or "immunosenescence". Systems Biology aims to the comprehensive knowledge of the structure, dynamics, control and design that define a give...
journal_title:Immunology letters
pub_type: 杂志文章,评审
doi:10.1016/j.imlet.2014.09.009
更新日期:2014-11-01 00:00:00
abstract::A variety of straightforward biochemical techniques have been applied to the analysis of HLA molecules. These techniques centre largely on two components: monoclonal antibodies (mAbs) and gel analysis systems. Although only the basic applications are discussed here, the techniques have broad applications. Monoclonal-g...
journal_title:Immunology letters
pub_type: 杂志文章,评审
doi:10.1016/0165-2478(91)90194-f
更新日期:1991-07-01 00:00:00
abstract::Two crucial mediators of monocyte activation by lipopolysaccharide (LPS) are the acute phase plasma factor, lipopolysaccharide binding protein (LBP) and cell-surface-expressed CD14. Whether macrophage (M phi) recognized and respond to LPS in a similar manner is unknown. Here we show that human monocyte-derived M phi r...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/s0165-2478(96)02645-4
更新日期:1996-12-01 00:00:00
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journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(93)90104-a
更新日期:1993-06-01 00:00:00
abstract::Multiple sclerosis (MS) is a debilitating neurological disorder of the central nervous system (CNS), characterized by activation and infiltration of leukocytes and dendritic cells into the CNS. In the initial phase of MS and its animal model, experimental autoimmune encephalomyelitis (EAE), peripheral macrophages infi...
journal_title:Immunology letters
pub_type: 杂志文章,评审
doi:10.1016/j.imlet.2014.03.006
更新日期:2014-07-01 00:00:00
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journal_title:Immunology letters
pub_type: 杂志文章,评审
doi:10.1016/0165-2478(90)90040-w
更新日期:1990-06-01 00:00:00
abstract::CBA spleen cells have been stimulated in vitro with A/Jap influenza virus-infected CBA spleen cells to generate a 'primary' cytotoxic lymphocyte (CL) response. The culture conditions were devised to allow the segregation of individual clones of CL and cytotoxicity measured by the lysis of infected or non-infected L-92...
journal_title:Immunology letters
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更新日期:1982-01-01 00:00:00
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journal_title:Immunology letters
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更新日期:1996-11-01 00:00:00
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journal_title:Immunology letters
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更新日期:2017-09-01 00:00:00
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journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2010.01.004
更新日期:2010-03-10 00:00:00
abstract::We have reported that a cell line, KML1, obtained from lymph node cells of a 16-week-old female MRL/Mp-lpr/lpr (MRL/1) mouse produces a soluble factor(s) that promotes formation of anti-single-stranded (ss) DNA antibodies by cultures of spleen cells from old MRL/l mice (greater than 16 weeks old). Detailed examination...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(90)90035-o
更新日期:1990-03-01 00:00:00
abstract::Previous studies have demonstrated that culture supernatants of LPS-stimulated murine splenic B lymphocytes (BLPSSN) are able to inhibit the growth of freshly isolated B cells via an IgM antibody. In this work we investigated the progress of LPS-activated B lymphocytes through the cell cycle in the presence of this an...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(92)90070-5
更新日期:1992-08-01 00:00:00
abstract::The observation that protective immunity induced by immunization with radiation attenuated Plasmodium berghei and Plasmodium yoelii sporozoites is dependent on CD8+ T lymphocytes in some strains of mice led us to speculate that immunization with sporozoites induces cytotoxic T lymphocytes (CTL) that recognize malaria ...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(90)90087-7
更新日期:1990-08-01 00:00:00
abstract::In spite of impressive results in the treatment of acute lymphoblastic B cell leukemia (B-ALL) with chimeric antigen receptor (CAR) T cells, the clinical outcome of some hematological cancers like follicular lymphoma (FL) and chronic lymphocytic leukemia (CLL) has not been very promising likely due to immunosuppressiv...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2020.04.005
更新日期:2020-07-01 00:00:00
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journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(83)90063-9
更新日期:1983-01-01 00:00:00
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journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/s0165-2478(97)00057-6
更新日期:1997-06-01 00:00:00
abstract::The physiological role(s) of fetuin, a protein present in plasma and many tissues of developing animals at levels much higher than in the adult, is unknown. Here we show that fetuin can modify the responses of macrophages to lipopolysaccharide (LPS) stimulation. At concentrations of fetuin in the medium, corresponding...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/s0165-2478(97)00126-0
更新日期:1998-01-01 00:00:00
abstract::Rheumatic fever (RF) and chronic rheumatic heart disease (RHD) are complications of oropharyngeal infection caused by Streptococcus pyogenes. Despite the importance of the complement system against infections and autoimmunity diseases, studies on the role of the lectin pathway in RF and RHD are scarce. Thus, our aim w...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2020.11.006
更新日期:2021-01-01 00:00:00